The effect of dried bottle gourd and pumpkin as hepatoprotective agent from acrylamide toxicity in experimental rats

Abstract

crylamide (ACR) is found in foods containing carbohydrates and proteins,where it is formed during the thermal processing. It is classified as neurotoxic and probably carcinogenic to humans. The present study investigated the effect of dried pumpkin and bottle gourd versus acrylamide induced oxidative stress and hepatotoxicity in male albino rats. The rats were divided into equal six groups. The first group (6 rats) were fed on basal diet and served as negative control. The all other groups were injected intraperitoneal (i.p.) with acrylamide (50 mg/kg body weight) for 28 days. The second group fed on basal diet and served as a positive control group. The third and fourth groups fed on basal diet supplemented with bottle gourd fruits powder (5%) and (10%). The fifth and sixth groups fed on basal diet added with pumpkin fruits powder (5%) and (10%). The chemical composition and phenolic compounds of both fruits were done. At the end of the experiment biological data were calculated, blood samples were taken to biochemical analysis. In addition, liver tissues were analyzed for antioxidant markers, malondialdehyde (MDA), as well as histological examination was done. The results revealed that acrylamide group increased liver weight, liver functions, serum lipid profile, liver MDA and NO, decreased in serum HDL-C, Liver GPX, SOD and CAT. All treated groups with two fruits showed improvement previously parameters compared with positive control group. In conclusion, the consumption of pumpkin and bottle gourd fruits powder can lower the side effects of acrylamide toxicant.