The effect of doxazosin on platelet aggregation in normotensive subjects and patients with hypertension: An in vitro study

Hernández, R.; Carvajal, Atiff R.; Pajuelo, Jaime; Hernández, María José; Padilla, María Cristina Armas; Barragan, Oscar; Boada, J; Roa, Emir

doi:10.1016/0002-8703(91)90877-k

Cited by 29 publications

(8 citation statements)

References 11 publications

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“…Hyperactive platelets produce more reactive oxygen species (ROS), enhancing platelet activity by decreasing NO bioavailability and lowering the intracellular concentration of Ca 2+ [125]. Furthermore, excessive platelet activation is also attributed to the high mechanical shear forces in the circulation, reduced blood flow, and vascular damage, which is observed in patients with hypertension and diabetes [126]. Platelet hyperactivity plays a causal role in triggering and maintaining the pro-inflammatory and pro-thrombotic state of obesity, creating an environment favorable for atherothrombotic vascular events.…”

Section: Platelet Hyperactivity Is An Important Contributor To the Dementioning

confidence: 99%

Role of Gut Microbiota and Their Metabolites on Atherosclerosis, Hypertension and Human Blood Platelet Function: A Review

Duttaroy

2020

Preprint

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Emerging data have demonstrated a strong association between the gut microbiota and the development of cardiovascular disease (CVD) risk factors such as atherosclerosis, inflammation, obesity, insulin resistance, platelet hyperactivity, and plasma lipid abnormalities. Several studies in humans and animal models have demonstrated an association between gut microbial metabolites, such as trimethylamine-N-oxide (TMAO), short-chain fatty acids, and bile acid metabolites, amino acid breakdown products, with CVD. Human blood platelets are a critical contributor to the hemostatic process. Besides, these blood cells play a crucial role in developing atherosclerosis and, finally, contribute to cardiac events. Since the TMAO, and other metabolites of the gut microbiota, are associated with platelet hyperactivity, lipid disorders, and oxidative stress, the diet-gut microbiota interactions have become an important research area in the cardiovascular field. Platelets became hyperactive in people with diabetes mellitus, sedentary lifestyle, obesity, and insulin resistance and exhibited increased sensitivity at a baseline level and in response to agonists, ultimately contributing to increased aggregation plaque development. In addition to these factors, TMAO also contributes to platelet hyperactivity. Several approaches are now suggested to reduce plasma TMAO levels, such as microbiota modulation using probiotics, prebiotics, and oral broad-spectrum antibiotics. This review describes the association between microbiota-derived metabolites and CVD development.

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Section: Platelet Hyperactivity Is An Important Contributor To the Dementioning

confidence: 99%

Role of Gut Microbiota and Their Metabolites on Atherosclerosis, Hypertension and Human Blood Platelet Function: A Review

Duttaroy

2020

Preprint

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“…Different cardiovascular risk factors are associated to platelet hyperactivity such as diabetes mellitus, obesity, hypertension, smoking, stress, and hypercholesterolemia (El Haouari and Rosado, ). Furthermore, excessive platelet activation is also attributed to the high mechanical shear forces in the circulation and vascular damage, which is observed in patients with hypertension and diabetes (Hernandez Hernandez et al ., ). Indeed, following blood vessel injury and/or atherosclerotic plaque erosion, platelets adhere by their surface receptors to the subendothelial matrix which triggers their activation and subsequent aggregation (Davi and Patrono, ; Watson et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Medicinal Plants with Antiplatelet Activity

Haouari

Rosado

2016

Phytother. Res.

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Blood platelets play an essential role in the hemostasis and wound-healing processes. However, platelet hyperactivity is associated to the development and the complications of several cardiovascular diseases. In this sense, the search for potent and safer antiplatelet agents is of great interest. This article provides an overview of experimental studies performed on medicinal plants with antiplatelet activity available through literature with particular emphasis on the bioactive constituents, the parts used, and the various platelet signaling pathways modulated by medicinal plants. From this review, it was suggested that medicinal plants with antiplatelet activity mainly belong to the family of Asteraceae, Rutaceae, Fabaceae, Lamiaceae, Zygophyllaceae, Rhamnaceae, Liliaceae, and Zingiberaceae. The antiplatelet effect is attributed to the presence of bioactive compounds such as polyphenols, flavonoids, coumarins, terpenoids, and other substances which correct platelet abnormalities by interfering with different platelet signalization pathways including inhibition of the ADP pathway, suppression of TXA2 formation, reduction of intracellular Ca(2+) mobilization, and phosphoinositide breakdown, among others. The identification and/or structure modification of the plant constituents and the understanding of their action mechanisms will be helpful in the development of new antiplatelet agents based on medicinal plants which could contribute to the prevention of thromboembolic-related disorders by inhibiting platelet aggregation. Copyright © 2016 John Wiley & Sons, Ltd.

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“…However, their hyperactivity is involved in the development and complications of several cardiovascular diseases, including thrombosis, atherosclerosis, myocardial infarction, peripheral artery disease, and ischemic stroke. Furthermore, excessive platelet activation is endorsed by the high mechanical shear forces in the circulation and vascular damage [7]. Indeed, following blood vessel injury and atherosclerotic plaque erosion, platelets adhere by their surface receptors to the subendothelial matrix, which triggers their activation and subsequent aggregation [8,9].…”

Section: Introductionmentioning

confidence: 99%

Bioanalytical Rp-HPLC Method Development and Validation of Clopidogrel Bisulfate in Wistar Rat Plasma and Its Application to Pharmacokinetic Study

2022

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Objective: A novel, simple, precise, accurate, sensitive, and reproducible HPLC method for determining clopidogrel bisulfate in Wistar rat plasma was developed and validated. Methods: The chromatographic separation was performed using Xterra C18 (250 x 4.6 mm, 5μ) column. Mobile phase composed of Acetonitrile ACN: 0.05M potassium dihydrogen orthophosphate buffer pH 4.2 and in the ratio of 75:25% v/v at a flow rate of 1.2 ml/min. Detection was carried out using a PDA detector at 220 nm. The bioanalytical clopidogrel method was validated as per ICH guidelines. Results: The selected chromatographic condition was found to efficiently separate clopidogrel bisulfate (RT-2.838 min). The calibration curve was linear over the concentration range 40-200 ng/ml in Wistar rat plasma with a correlation coefficient of 0.999, respectively. The precision study revealed that the cumulative percentage variation was within the acceptable limit, and accuracy research showed the value of mean percent recovery between 99.72-99.83 %. Conclusion: A simple, rapid, specific, accurate, and precise analytical method was developed and validated using Wistar rat plasma. The technique was strictly validated according to the ICH guidelines. Acquired results demonstrate that the proposed strategy can be effortlessly and advantageously applied for routine analysis of clopidogrel in the Wistar rat plasma.

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The effect of doxazosin on platelet aggregation in normotensive subjects and patients with hypertension: An in vitro study

Cited by 29 publications

References 11 publications

Role of Gut Microbiota and Their Metabolites on Atherosclerosis, Hypertension and Human Blood Platelet Function: A Review

Role of Gut Microbiota and Their Metabolites on Atherosclerosis, Hypertension and Human Blood Platelet Function: A Review

Medicinal Plants with Antiplatelet Activity

Bioanalytical Rp-HPLC Method Development and Validation of Clopidogrel Bisulfate in Wistar Rat Plasma and Its Application to Pharmacokinetic Study

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