The Effect of Donor Variation and Senescence on Endothelial Differentiation of Human Mesenchymal Stromal Cells

Abstract

Tissue Engineering Part A The effect of donor variation and senescence on endothelial differentiation of human mesenchymal stromal cells (doi: 10.1089(doi: 10. /ten.TEA.2012 This article has been peer-reviewed and accepted for publication, but has yet to undergo copyediting and proof correction. The final published version may differ from this proof.

Activity of mesenchymal stem cells in therapies for chronic skin wound healing

“…72,75 Recently several groups have focused on differentiation of MSCs into endothelial cells, an approach that has potential to be useful in direct transplant into anti-angiogenic environments such as ischemic wound beds. Results have shown endothelial-like cell populations derived from human MSCs in vitro with varying degrees of donor variation, 76,77 while Bago et al showed similar results for amnion-derived MSCs in glioma tumors. 78 Furthermore, pericytes that stabilize vessel walls and promote vessel maturation during angiogenesis have been shown to be derived from bone marrow populations following injury.…”

MicroRNA Levels as Prognostic Markers for the Differentiation Potential of Human Mesenchymal Stromal Cell Donors

“…hMSCs were expanded up to passage 2 and used in passage 3 to test their differentiation potential. The determination of cell surface marker expression (CD105, CD11b, CD19, CD45, HLA-DR, CD90, CD73, CD34), osteogenic potential, adipogenic potential, and endothelial induction of the used hMSC donors is described elsewhere [22,23].…”

“…Changes in upstream regulators and biofunctions were visualized using Ingenuity Pathway Analysis software (IPA Ò ; Ingenuity Systems). In this study, the described data set was previously used by Portalska et al and Mentink et al [22,23]. We reanalyzed this data set after the formation of two groups: good and low chondrogenic performing hMSC donors.…”

“…All donor hMSCs expressed the anticipated CD marker profile of hMSCs [22,23]. We compared the chondrogenic potential in depth based on histological appearance, GAG deposition, and chondrogenic mRNA expression.…”

“…This cohort has previously been characterized in great detail with respect to the osteogenic, adipogenic, endothelial cell differentiation potential and CD marker expression [22,23] and meets the criteria proposed by the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy [24]. Similar to differentiation into other cell types, the chondrogenic potential varied significantly between donors.…”