The Effect of Donor Cornea Epithelium Removal on the Incidence of Allograft Rejection Reactions

Tuberville, Audrey W.; Foster, C. Stephen; Wood, Thomas O.

doi:10.1016/s0161-6420(83)34395-5

Cited by 43 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tuberville et al 20 championed this concept more than a decade ago and reported that human corneas deprived of epithelium enjoyed better survival in keratoplasty. However, this view was refuted by a similar study in human beings conducted by Stulting et al 21 We suspect, but have no direct information, that the physical method by which epithelium is removed from the donor cornea may have secondary consequences that are deleterious to graft survival.…”

Section: Sources Of Alloimmunogenicity Within the Corneamentioning

confidence: 99%

Immune Privilege and Immunogenicity Reside among Different Layers of the Mouse Cornea

Hori

Joyce

Streilein

2007

Ocular Immunology and Inflammation

View full text Add to dashboard Cite

Purpose: To determine the extent to which each layer of the mouse cornea displays alloimmuno-genicity or immune privilege. Methods: Intact corneas or individual or combined layers of corneas from normal or cauterized eyes of BALB/c, C57BL/6, and CD95L-deficient B6-gld mice were grafted beneath the kidney capsule of normal BALB/c, B10.D2, BALB.B mice or of BALB/c mice presensitized to donor antigens. Graft fate was assessed clinically and histologically and acquisition of donor-specific delayed hypersensitivity (DH) was assessed at selected intervals after grafting. Results: Full-thickness allogeneic corneas induced vigorous DH and were rejected acutely. Similar results were obtained with allografts of corneal epithelium alone (if supported by syngeneic viable stroma), allografts of epithelium from cauterized corneas (containing Langerhans' cells), and stromal allografts deprived of endothelium. Grafts comprised of stroma plus endothelium (without epithelium) were not rejected, nor did they induce DH unless the graft had no CD95L expression. If stroma-endothelium grafts had no CD95L expression, DH directed against major histocompatibility complex (MHC), but not minor histocompatibility, alloantigens was induced. Moreover, CD95L expressed on stroma-endothelium grafts protected endothelial cells, but not stromal cells, from rejection in presensitized recipients. Conclusions: When grafted to a heterotopic site, the alloimmunogenicity of the normal cornea resides within its epithelial and stromal layers, whereas immune privilege arises from the endothelium. In normal mice, CD95L-expressing endothelium can inhibit the stroma from inducing immunity directed at MHC alloantigens, but in presensitized mice the endothelium can protect itself only from immune rejection.

show abstract

Section: Sources Of Alloimmunogenicity Within the Corneamentioning

confidence: 99%

Immune Privilege and Immunogenicity Reside among Different Layers of the Mouse Cornea

Hori

Joyce

Streilein

2007

Ocular Immunology and Inflammation

View full text Add to dashboard Cite

show abstract

“…This suggests that host sensitization and subsequent graft rejection could be modified by removing donor epithelium prior to corneal transplantation. Different clinical studies with altogether 439 corneal grafts have failed to clarify whether removing epithelium affects the incidence of human corneal allograft rejection [19,23]. However, postoperative healing problems complicate the decision to remove graft epithelium.…”

Section: Introductionmentioning

confidence: 99%

Ballistic transfer of minimalistic immunologically defined expression constructs for IL4 and CTLA4 into the corneal epithelium in mice after orthotopic corneal allograft transplantation

Merediz

Zhang²,

Wittig

et al. 2000

Graefe's Archive for Clinical and Experimental Ophthalmology

View full text Add to dashboard Cite

show abstract

“…From experimental studies it appears that acute corneal graft rejection is associated with the presence of donor Langerhans cells (LCs) and that strategies that reduce or eliminate donor LCs prolong graft survival (Rubsamen et al, 1990 ;Tuberville, Foster and Wood, 1983). These findings are not surprising since LCs are the only corneal cells that constitutively express class II antigen.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Tissue Culture on Corneal Immunogenicity

Gabrić¹,

Dekaris²,

Šuman

et al. 1999

Experimental Eye Research

View full text Add to dashboard Cite

The Effect of Donor Cornea Epithelium Removal on the Incidence of Allograft Rejection Reactions

Cited by 43 publications

References 9 publications

Immune Privilege and Immunogenicity Reside among Different Layers of the Mouse Cornea

Immune Privilege and Immunogenicity Reside among Different Layers of the Mouse Cornea

Ballistic transfer of minimalistic immunologically defined expression constructs for IL4 and CTLA4 into the corneal epithelium in mice after orthotopic corneal allograft transplantation

The Influence of Tissue Culture on Corneal Immunogenicity

Contact Info

Product

Resources

About