The effect of Dodonaea viscosa var. angustifolia on Candida albicans proteinase and phospholipase production and adherence to oral epithelial cells

Patel, Mrudula; Gulube, Zandiswa; Dutton, Mike F.

doi:10.1016/j.jep.2009.05.002

Cited by 25 publications

(16 citation statements)

References 41 publications

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“…Plant such as Cassia spectabilis and Scutellaria baicalensis have been shown to inhibit biofilm formation by C. albicans [18, 19]. A previous study demonstrated that DVA extract inhibits adherence of C. albicans to host cells [20], and the present study shows that it also inhibits adherence to inert material. This has major implications for denture wearers and patients with head and neck cancers who wear prostheses.…”

Section: Discussionsupporting

confidence: 66%

Dodonaea viscosavar.angustifoliaInhibits Germ Tube and Biofilm Formation byC. albicans

Naicker

Patel

2013

Evidence-Based Complementary and Alternative Medicine

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The virulence factors of Candida albicans are germ tube and biofilm formation, adherence to host tissues, and production of hydrolytic enzymes. This study investigated the effect of Dodonaea viscosa var. angustifolia extract on the germ tube and biofilm formation of C. albicans. Serum containing the three subinhibitory concentrations of leaf extract was inoculated with C. albicans, incubated, and viewed under a light microscope. Number of cells with germ tube was recorded and the results were analysed using Scheffe test for pairwise comparison. Biofilms were grown on coverslips in the presence of plant extracts and processed for scanning electron microscopy (SEM). Planktonic cells were grown in the presence of plant extract for 6 h and processed for electron microscopy (TEM). The crude plant extract significantly (P < 0.01) reduced the germ tube formation of C. albicans at 3.125 (85.36%), 1.56 (61.91%), and 0.78 mg/mL (26.27%) showing a concentration dependent effect. SEM results showed concentration dependent reduction in biofilm and hyphae formation. TEM results showed that the plant extract caused damage to the cell wall and cell membrane. DVA extract has ability to reduce virulence of C. albicans by inhibiting germ tube and biofilm formation through damage to the cell wall. Therefore, it has therapeutic potential.

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Section: Discussionsupporting

confidence: 66%

Dodonaea viscosavar.angustifoliaInhibits Germ Tube and Biofilm Formation byC. albicans

Naicker

Patel

2013

Evidence-Based Complementary and Alternative Medicine

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“…In addition S. mutans that may survive after exposure to the plant may not be able to adhere to the acquired pellicle and form biofilm. DVA is known to inhibit the adherence of C. albicans to oral epithelial cells, which is an important virulence factor in the pathogenesis of oral candidiasis (Patel et al, 2009b). These results are particularly applicable to immunocompromised individuals who are predisposed to oral candidiasis.…”

Section: Discussionmentioning

confidence: 96%

“…angustifolia Benth by a taxonomist, from the Herbarium at the University of the Witwatersrand. Voucher specimens number J 94882, were deposited previously in this Herbarium (Patel et al, 2009b).…”

Section: Plant Materialsmentioning

confidence: 99%

“…DVA also has antiviral activity against HIV types 1 and 2 (Asres et al, 2001). Its anticandida activity has also been shown, especially the ability to inhibit the adherence of Candida albicans to oral epithelial cells thereby inhibiting the initial stage of colonization and infection (Patel et al, 2009b). To date, little is known about the effect of this plant on S. mutans.…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory activity of Dodonaea viscosa var. angustifolia extract against Streptococcus mutans and its biofilm

Naidoo

Patel

Gulube

et al. 2012

Journal of Ethnopharmacology

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“…Anticandidal activity in crude acetone-based extract of the leaves of D. viscosa has been reported when forty clinical isolates of C. albicans including twenty each from HIV-positive and HIV-negative patients and a separate control strain, with MIC range of 6.25 to 25 µg/ml (Patel and Coogan, 2008). Extract from the leaves was also found to inhibit the adherence of C. albicans to oral epithelial cell (Patel et al, 2009), and since adherence is the preliminary step of any microbial invasion, therefore, it has the ability to cause impact upon the crucial colonization step of an infection process.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical screening and in vitro evaluation of anticandidal activity of Dodonaea viscosa (L.) Jaeq. (Sapindaceae)

Khurram¹,

Hameed²,

Amin³

et al. 2011

Afr. J. Pharm. Pharmacol.

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The activity of fractions derived from hydroalcoholic extract of Dodonaea viscosa leaves against Candida albicans (Cl. I. 4043) was evaluated. The hydroalcoholic extract was sequentially fractionated to give n-hexane, dichloromethane, ethylacetate and n-butanol fractions that were subjected to qualitative phytochemical analyses. Disk diffusion assay was used in preliminary anticandidal screening with clotrimazole and chloroform serving as positive and negative controls, respectively. Optimized solvent systems were used for thin layer chromatography (TLC) that was followed by contact bioautography to evaluate the bioactivities of the fractions. Using broth microdilution technique, minimum inhibitory concentrations (MIC) of the individual fractions were established. With the exception of aqueous fraction all the fractions exhibited anticandidal activities (zone of inhibition 10 mm) in preliminary screening against test yeast. However, n-hexane fraction showed two inhibition zones at R f = 0.14 and 0.60 in contact bioautography, which indicates location of inhibitory compounds. The MIC of 62.5 µg/ml also supports the presence of anticandidal moieties in n-hexane fraction. Flavonoids, terpenoids, tannins and steroids were the main metabolites indicated in phytochemical screenings.

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The effect of Dodonaea viscosa var. angustifolia on Candida albicans proteinase and phospholipase production and adherence to oral epithelial cells

Cited by 25 publications

References 41 publications

Dodonaea viscosavar.angustifoliaInhibits Germ Tube and Biofilm Formation byC. albicans

Dodonaea viscosavar.angustifoliaInhibits Germ Tube and Biofilm Formation byC. albicans

Inhibitory activity of Dodonaea viscosa var. angustifolia extract against Streptococcus mutans and its biofilm

Phytochemical screening and in vitro evaluation of anticandidal activity of Dodonaea viscosa (L.) Jaeq. (Sapindaceae)

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