Phytochemical screening and in vitro evaluation of anticandidal activity of Dodonaea viscosa (L.) Jaeq. (Sapindaceae)

Abstract

The activity of fractions derived from hydroalcoholic extract of Dodonaea viscosa leaves against Candida albicans (Cl. I. 4043) was evaluated. The hydroalcoholic extract was sequentially fractionated to give n-hexane, dichloromethane, ethylacetate and n-butanol fractions that were subjected to qualitative phytochemical analyses. Disk diffusion assay was used in preliminary anticandidal screening with clotrimazole and chloroform serving as positive and negative controls, respectively. Optimized solvent systems were used for thin layer chromatography (TLC) that was followed by contact bioautography to evaluate the bioactivities of the fractions. Using broth microdilution technique, minimum inhibitory concentrations (MIC) of the individual fractions were established. With the exception of aqueous fraction all the fractions exhibited anticandidal activities (zone of inhibition 10 mm) in preliminary screening against test yeast. However, n-hexane fraction showed two inhibition zones at R f = 0.14 and 0.60 in contact bioautography, which indicates location of inhibitory compounds. The MIC of 62.5 µg/ml also supports the presence of anticandidal moieties in n-hexane fraction. Flavonoids, terpenoids, tannins and steroids were the main metabolites indicated in phytochemical screenings.

A review on Dodonaea viscosa: A potential medicinal plant

“…With the exception of aqueous fraction, all the fractions exhibited anticandidal activities (zone of inhibition ≥ 10 mm). The MIC of n-hexane fraction was 62.5 μg/ml [106]. The in vitro antiviral activity of different extracts from Dodonaea viscosa leaves was studied against coxackievirus B3 (CVB3) and rotavirus SA-11 (RV SA-11) infections.…”

Evaluation of In Vivo Antidiarrheal Activities of Hydroalcoholic Leaf Extract of Dodonaea viscosa L.(Sapindaceae) in Swiss Albino Mice

“…22 The D viscosa leaves have been evaluated for its various activities and provided promising effects. Accordingly, the plant leaves demonstrated antidiabetic effects against alloxan-induced diabetes in rabbits and rats, 23,24 antibacterial activities against selected gram-positive bacteria (Bacillus subtilis, Bacillus cereus, Micrococcus luteus, and Staphylococcus aureus), and gram-negative bacteria (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa), 25 antifungal activities against different species of fungi, 26,27 significant anti-inflammatory effect against the paw edema induced by carrageenan injection, 28 and antinociceptive activity in rats and mice against experimental pain models of glacial acetic acidinduced writhing, hot plate, and tail flick. 29 The plant extract has also exhibited antiulcer activity against ethanol and indomethacin-induced gastric ulcer in Wistar rats, 30 as well as an antidiarrheal effect against castor oil-induced diarrhea in mice in which alcohol and aqueous extracts of the roots of the plant were significantly reduced diarrhea in mice.…”