The effect of DMA level on morphology and fertilising ability of Japanese quail (Coturnix japonica) spermatozoa

Chełmońska, B.; Łukaszewicz, Ewa; Kowalczyk, Artur; Jerysz, Anna

doi:10.1016/j.theriogenology.2005.06.002

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…Generally, the types of observed forms and their percentage were similar to other domesticated ( Gallus domesticus , Numida meleagris , Meleagris gallopavo ) and wild Galliformes . The amount of live normal cells, which posses the highest potency to fertilize the ova, varying in our experiment from 45.0 to 85.3%, was comparable to 56.4–94.0% observed in semen of G. domesticus [Siudzińska and Łukaszewicz, 2008; Łukaszewicz et al, 2008]; Coturnix coturnix japonica (45.2–55.7%) [Chełmońska et al, 2006]; M. gallopavo (64.0%) and N. meleagris (69.0%) [Blesbois et al, 2005]. However, in the Phasianidae family the percentage of these desirable cells was higher: 70% in T. temminicki ; 65.9–68.9% in T. caboti [Saint Jalme et al, 2003] and P. colchicus mongolicus semen [Marzoni et al, 2000].…”

Section: Discussionsupporting

confidence: 78%

Characteristics of fresh semen of captive‐bred capercaillie Tetrao urogallus L.

Łukaszewicz

Kowalczyk

Rzońca³

2010

Zoo Biology

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In Poland Capercaillie (Tetrao urogallus L.) is one of the most seriously endangered grouse species. The ability of semen collection and its utilization for Capercaillie female insemination would allow overcoming some fertility problems observed in captive-bred populations and thus reduce the rate of loss of genetic diversity. The present experiment was carried out on 13 individuals: eight males were kept with females and five alone. From each male, semen was collected four times, every second day, and overall semen appearance (color, viscosity), ejaculate volume, spermatozoa concentration, motility and morphology were examined. Ejaculates suitable for artificial insemination (AI) were obtained from 11 individuals. The volume of ejaculates varied from one drop (noted as 0.010 ml) to 0.180 ml, whereas spermatozoa concentration varied from 100 × 10(6) ml(-1) to 1950 × 10(6) ml(-1). The total amount of live spermatozoa for males kept with females varied from 82.0 to 98.3% (92.9% on average) and among them, from 38.7 to 82.0% were morphologically normal (67.6% on average), whereas for solitary males these values were the following: from 93.7 to 98.7 of total live (96.3% on average) and from 45.0 to 85.3% live normal cells (65.7% on average). No significant group effect was observed for above traits. Semen from males kept with females contained significantly (P<0.01) fewer cells with bulb head (12.2% vs. 21.6%), but higher numbers of bent neck spermatozoa (3.0 vs. 2.1%) and with other deformities (10.0 vs. 6.8%); however, for last two forms existing differences were not significant. Results obtained indicate the possibility of collecting valuable ejaculates from captive-bred Capercaillie, both kept with or without females, which makes possible the application of AI in order to increase the progeny number and gene exchange of this species across time and geographical distance.

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Section: Discussionsupporting

confidence: 78%

Characteristics of fresh semen of captive‐bred capercaillie Tetrao urogallus L.

Łukaszewicz

Kowalczyk

Rzońca³

2010

Zoo Biology

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“…Cryoprotectants agents can exert deleterious effects on sperm morphology as was verified by Chełmoñska, Łukaszewicz, Kowalczyk and Jerysz (2006) for Japanese quail semen. Methanol and DMSO have already been tested as cryoprotectants in the cooling storage of the curimba semen by Franciscatto, Murgas, Miliorini, Silva and Logato (2002).…”

Section: Introductionmentioning

confidence: 81%

A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation

Miliorini

Murgas

Rosa

et al. 2010

Aquaculture Research

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The present study evaluated some cryoprotectants and concentrations for curimba (Prochilodus lineatus) semen preservation from a simple methodology of analysis of sperm morphology. Semen of nine males was diluted (1:4) into eight cryosolutions, all of them containing 5% of BTS™ Minitüb, still comprising methanol or dimethyl sulphoxide (DMSO) at four concentrations: 5%, 7.5%, 10% and 12.5%. Morphological sperm analysis was performed on an optical microscope at × 1000 magnification. Damages on the head, midpiece and tail rest (the main and terminal portions) were investigated and classified as primary or secondary, according to the assumed influence on fertilization. There was a linear reduction in the total damages with an increase in the cryoprotectant concentration. Samples with DMSO showed the lowest percentage of fractured tails and tail stumps. The cryopreservation protocol retained the fertilizing potential of the sperm after freezing and both methanol 5% and DMSO 7.5% conferred adequate protection to the curimba sperm cells. Sperm morphology, as pointed out here, must be incorporated into routine fish sperm (cryopreserved or not) analysis once sperm defects influence fertilization directly. To the best of our knowledge, this was the first report on curimba sperm damages after cryopreservation by optical microscopy. The classification model presented in this study can be adequate to tackle fish sperm damages.

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“…Despite decades of extensive research on the use of permeating cryoprotectants (Sexton, 1975;Oderkirk and Buckland, 1977;Sexton, 1980;Tselutin et al, 1999;Blanco et al, 2000;Tai et al, 2001;Chełmońska et al, 2006;Kowalczyk, 2008), survival of avian sperm after freezing/thawing procedures remains highly variable. Sperm cryosurvival has been improved in various mammalian 0378-4320/$ -see front matter.…”

Section: Introductionmentioning

confidence: 99%

Comparative cryopreservation of avian spermatozoa: Benefits of non-permeating osmoprotectants and ATP on turkey and crane sperm cryosurvival

Blanco

Long

Gee³

et al. 2011

Animal Reproduction Science

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A comparative approach was used to evaluate the cryosurvival of turkey and crane sperm frozen in a dimethylacetamide (DMA) cryodiluent supplemented with osmoprotectants and ATP. A range (6-26%) of DMA concentrations was used alone or in combination with ATP (30, 60 or 118mM) or one of the following osmoprotectants: (1) sucrose (turkey, 8.0%; crane, 5.0%); (2) 5.0% sucrose and 5.0% trehalose; or (3) betaine hydrochloride (0.1, 0.2 or 0.4mM). The viability of thawed sperm was assessed using the nigrosin-eosin stain and sperm motility was determined using the hanging-drop technique. For semen frozen only with DMA, post-thaw sperm motility was greatest (P<0.05) for the 6.0%, 10.0% and 18% concentrations, regardless of species. Turkey sperm frozen with the sucrose/trehalose combination had greater (P<0.05) post-thaw motility for all DMA treatments compared to DMA alone. The lowest concentration of the osmoprotectant betaine hydrochloride substantially improved turkey sperm viability post-thaw in all treatments compared to DMA alone (P<0.05). The post-thaw motility of crane sperm was improved (P<0.05) with a combination of 18.0%, 24.0% or 26.0% DMA and 30mM ATP. Moreover, in the presence of osmoprotectants, crane sperm motility decreased as the osmoprotectant concentration increased. The lowest concentration of ATP also improved crane sperm viability post-thaw, especially for DMA concentrations 18% or greater. The combination of sucrose and trehalose improved (P<0.05) crane sperm viability only with 6% and 10% DMA. These data affirm that there are avian-specific differences in sperm survival after cryopreservation and suggest that post-thaw survival can be enhanced by including species-based osmoprotectant/ATP combinations in a cryodiluent where DMA is the cryoprotectant.

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The effect of DMA level on morphology and fertilising ability of Japanese quail (Coturnix japonica) spermatozoa

Cited by 9 publications

References 19 publications

Characteristics of fresh semen of captive‐bred capercaillie Tetrao urogallus L.

Characteristics of fresh semen of captive‐bred capercaillie Tetrao urogallus L.

A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation

Comparative cryopreservation of avian spermatozoa: Benefits of non-permeating osmoprotectants and ATP on turkey and crane sperm cryosurvival

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