The effect of dilution rate and successive semen collections on sperm quality and sexual motivation of sexually mature South African Merino rams

Bonato, Maud; Smith, Marna; Malecki, Irek; Cloete, S.W.P.

doi:10.1007/s11250-021-02627-0

Cited by 9 publications

(8 citation statements)

References 51 publications

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“…(5)(6)(7)(8). Кроме того, определенное значение имеют репродуктивный менеджмент, профессионализм техника по получению спермы, метод и частота отбора спермы (9)(10)(11).…”

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Sexual Activity and Sperm Production of Charolais and Ile-De-France Rams in Different Seasons of the Year

Мамонтова¹

2021

S-h. biol.

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Sexual Activity and Sperm Production of Charolais and Ile-De-France Rams in Different Seasons of the Year

Мамонтова¹

2021

S-h. biol.

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“…These findings are in agreement with previous studies showing similar or higher fertility rates after cervical AI with fresh semen in various sheep breeds. More precisely, a fertility rate of 65% in Argentine and South Africa has been reported in Merino sheep using fresh diluted semen (Bonato et al, 2021; Fernandez et al, 2019). In another study, Ben Moula et al (2023) have recently reported a lower fertility rate of 61% in Sardi sheep using semen stored in SM at 5°C for 5 h. However, Simonetti et al (2002) reported a greater value (up to 76.47% fertility) while using fresh diluted semen.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of Duragen® and skim milk extenders to enhance ram semen quality and fertility: A promising alternative in ovine artificial insemination

El Amiri,

Ben Moula,

El Khelfaoui

et al. 2023

Reprod Domestic Animals

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This study aimed to investigate the effectiveness of Duragen® and skimmed milk (SM) extenders on the quality parameters, bacterial load and fertilization ability of stored ram semen. A total of 50 ejaculates from Sardi rams (n = 5) aged 2.5–3 years, were collected and stored in Duragen® and SM at 15°C. The motilities and velocity parameters generated by the CASA system were then evaluated at 0, 8 and 24 h of storage. Afterward, bacterial loads of sperm extended in Duragen® and SM were determined at 0, 5 and 24 h of incubation. In addition, ewes (n = 100) aged 2 years, have been chosen in the same herd. The selected ewes were then synchronized and inseminated using semen extended in Duragen® and SM and stored for 5 h at 15°C. The results revealed that total and progressive motilities, straight velocity (VSL), straightness (SRT), lateral head displacement (ALH) and beat cross frequency (BCF) were not affected by the extender type after 24 h of storage (p > .05). However, curvilinear velocity (VCL), velocity average path (VAP), linearity (LIN) and wobble (WOB) showed higher values in Duragen® compared with SM extender after 24 h of storage (p < .05). Bacterial loads were observed mainly in sperm stored in SM at 5 h (183 UFC/mL) and at 24 h (357 UFC/mL) of incubation. However, the only case showing a bacterial load in Duragen® is when the storage time attains 24 h (199 UFC/mL). Concerning fertility, sperm diluted in both extenders resulting in high fertility rates which reaches 66% and 73% for Duragen® and SM, respectively, with no statistical difference (p > .05). In summary, Duragen® extender decreased bacterial load in stored semen and maintained high ram sperm quality and fertility. These findings suggest that Duragen® extender could be used as SM alternative in ovine artificial insemination (OAI).

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“…This may be due to the high concentration of spermatozoa diluted by a factor of two times, the excessive accumulation of metabolic waste and the inability of the buffer material in the diluent to maintain its balance [ 22 ]. On the other hand, it may be caused by the rapid consumption of nutrients such as sugar in the diluent [ 10 ]. In experiment I, the post-thawed spermatozoa TM and PM of the 1:4 group were significantly lower ( p < 0.05) than those of the 1:2 group.…”

Section: Discussionmentioning

confidence: 99%

“…During the preservation of semen, the metabolism of spermatozoa continuously consumes nutrients and produces metabolic wastes and oxidative factors, which can affect the effectiveness of semen preservation. Therefore, when the spermatozoa concentration is too high, the spermatozoa density exceeds the capacity of the diluent to provide sufficient nutrients for sperm movement, and the buffer and antioxidant substances in the diluent are unable to maintain pH stability and redox balance [ 10 ]. When the concentration of spermatozoa is too low, the level of beneficial substances in seminal plasma decreases.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation

Zhang,

Wang,

Jiang

et al. 2024

Animals

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The dilution method and ratio were tested to assess their effects on the Hu ram semen after cryopreservation. Experiment I aimed to explore the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I (Tris-based and egg yolk) under the condition of 1:1 dilution of diluent II (diluent I and glycerol) on the Hu ram semen preserved in liquid nitrogen regarding spermatozoa motility and kinetic parameters. Experiment II aimed to investigate the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I under the condition of 1:2 dilution of diluent II to the Hu ram semen for cryopreservation on spermatozoa motility and kinetic parameters. The purpose of experiment III is to assess the effect of various dilution methods and ratios on the cryopreservation of Hu ram semen by detecting spermatozoa motility, kinetic parameters, plasma membrane integrity, acrosome integrity and reactive oxygen species (ROS) level. Experiment III includes four groups: one-step dilution method and two-step dilution method. The two-step dilution method includes two groups: 1:2, 1:1 and 1:3, 1:2, and the one-step dilution method includes two groups: 1:5 and 1:11. The results indicated that the post-thawed spermatozoa total motility (TM), progressive motility (PM) and average motion degree (MAD) were highest in the 1:2 group and significantly higher (p < 0.05) than those in the 1:1 and 1:4 groups under the condition of 1:1 dilution of diluent II. The post-thawed spermatozoa TM and PM of the 1:3 group were significantly higher (p < 0.05) than those of the other groups under the condition of 1:2 dilution of diluent II. The post-thawed spermatozoa TM, PM, plasma membrane integrity and acrosome integrity of the two-step group (1:3, 1:2) were the highest and significantly higher (p < 0.05) than those in the other groups. Additionally, the post-thawed spermatozoa ROS level of the two-step group (1:3, 1:2) was significantly lower (p < 0.05) than that in the one-step groups (1:5 and 1:11). Therefore, a two-step dilution (1:3, 1:2) was found to be the most suitable method and ratio for diluting the Hu ram semen after cryopreservation.

show abstract

The effect of dilution rate and successive semen collections on sperm quality and sexual motivation of sexually mature South African Merino rams

Cited by 9 publications

References 51 publications

Sexual Activity and Sperm Production of Charolais and Ile-De-France Rams in Different Seasons of the Year

Sexual Activity and Sperm Production of Charolais and Ile-De-France Rams in Different Seasons of the Year

Comparative evaluation of Duragen® and skim milk extenders to enhance ram semen quality and fertility: A promising alternative in ovine artificial insemination

Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation

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