The Effect of Different Temperatures and Durations on the Dormancy Breaking of Black Locust (Robinia pseudoacacia L.) and Honey Locust (Gleditsia triacanthos L.) Seeds

Başbağ, Mehmet; Aydın, Ali; Ayzit, Davut

doi:10.15835/nsb244690

Cited by 6 publications

(6 citation statements)

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“…The needle puncture or blade cut is frequently cited in the literature as effective in breaking physical dormancy of black locust, Fabaceae or other physically dormant seeds (Singh et al ., 1991; Funes and Venier, 2006; Masaka and Yamada, 2009; Cierjacks et al ., 2013; Abudureheman et al ., 2014; Giuliani et al ., 2015), so that it can be used as a reference method. The absence of germination following dry-heat treatments contradicted previous studies that reported 90–94% germination (Masaka and Yamada, 2009; Basbag et al ., 2010). Hot water soaking is proposed by forest managers to break physical dormancy (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Seeds were stored in paper bags in a cold room between 0 and 5°C (Bonner and Karrfalt, 2008; Cierjacks et al ., 2013). Two hundred and ten seeds from one tree of the Gabarnac population (Gironde, France) were used to test seven dormancy-breaking treatments, with 30 seeds per treatment: puncturing (P), consisting of making one pin puncture in the seed coat, using a needle; dry-heat (Basbag et al ., 2010) was applied by placing seeds in a Petri dish in an oven at 90°C for 20 and 30 min (H20, H30); imbibition by soaking in water (W) was performed in one cycle, placing seeds in nearly boiling water at 95°C (Turner and Dixon, 2009) and letting them cool for 15 h; finally we tested a novel sanding treatment (Fig. 1) using an automated grinder (2010 Geno/Grinder, SPEX SamplePrep, Metuchen, NJ, USA) that allows shaking of tubes controlling both intensity and duration.…”

Section: Methodsmentioning

confidence: 99%

“…Thus we decided not to consider it in our search for eco-friendly methods. Specific tests on black locust seeds were proven contradictory regarding their efficiency, for example, heat being selected by some authors whereas soaking in water or acid being indicated as the most efficient by others (Singh et al ., 1991; Masaka and Yamada, 2009; Basbag et al ., 2010; Giuliani et al ., 2015).…”

Section: Introductionmentioning

confidence: 99%

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Using automated sanding to homogeneously break seed dormancy in black locust (Robinia pseudoacaciaL., Fabaceae)

et al. 2017

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Physical dormancy of Robinia pseudoacacia seeds makes it a challenge for scientists and forest managers to obtain a homogeneous germination for larger seed samples. Water imbibition of the seeds can be achieved through manual piercing of the seed coat, but this method remains time consuming and heterogeneous. We tested several ecologically friendly methods to break seed dormancy, including manual pin puncture, water soaking, oven dry-heating (two temperatures) and sanding. Sanding was performed using an automatic grinder to control shaking duration (three durations) and get a homogeneous scraping of the coat. All methods, except dry-heating, resulted in successful dormancy breaking; water soaking was the least efficient method, attaining 57% germination. Sanding proved to be as efficient as puncturing (97%) but long duration sanding (10 or 15 min) could damage cotyledons, which would impede further development of the plant. Short-time sanding (5 min) proved to be the best method to reach high total germination and healthy (undamaged cotyledon) seedlings, and was successfully applied to 500 seeds. The reference puncture method and the automatic sanding were also tested on seeds of nine Fabaceae species and proved to be efficient for some species. Automated sanding can thus be used as a standard to break physical dormancy of black locust or other Fabaceae seeds to allow further comparative studies of plant populations or genotypes.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Using automated sanding to homogeneously break seed dormancy in black locust (Robinia pseudoacaciaL., Fabaceae)

et al. 2017

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“…Given that R . pseudoacacia seeds present dormancy [ 66 ], we assessed germination with one set of untreated seeds and another set of scarified seeds (1 minute in 90°C water + 24h soaked; [ 67 ]) to estimate the maximum potential germination. A seed was considered germinated when radicle emergence was observed.…”

Section: Methodsmentioning

confidence: 99%

Comparing the Sexual Reproductive Success of Two Exotic Trees Invading Spanish Riparian Forests vs. a Native Reference

Cabra-Rivas

Castro‐Díez

2016

PLoS ONE

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A widely accepted hypothesis in invasion ecology is that invasive species have higher survival through the early stages of establishment than do non-invasive species. In this study we explore the hypothesis that the sexual reproductive success of the invasive trees Ailanthus altissima (Mill.) Swingle and Robinia pseudoacacia L. is higher than that of the native Fraxinus angustifolia Vahl., all three species coexisting within the riparian forests of Central Spain. We compared different stages of the early life cycle, namely seed rain, seed infestation by insects, seed removal by local fauna, seed germination under optimal conditions and seedling abundance between the two invasive trees and the native, in order to assess their sexual reproductive success. The exotic species did not differ from the native reference (all three species displaying high seed rain and undergoing seed losses up to 50% due to seed removal by the local fauna). Even if the exotic R. pseudoacacia showed a high percentage of empty and insect-parasited seeds along with a low seedling emergence and the exotic A. altissima was the species with more viable seeds and of higher germinability, no differences were found regarding these variables when comparing them with the native F. angustifolia. Unsuitable conditions might have hampered either seedling emergence and survival, as seedling abundance in the field was lower than expected in all species -especially in R. pseudoacacia-. Our results rather suggest that the sexual reproductive success was not higher in the exotic trees than in the native reference, but studies focusing on long-term recruitment would help to shed light on this issue.

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“…Given that R. pseudoacacia seeds present dormancy [19], we used two sets of seeds for germination tests, one untreated and the other pretreated, to break dormancy. Based on previous experiments and studies [54], the best pretreatment consisted of scarifying them with boiling water (90 ∘ C) for one minute, subsequently removing them from heat and keeping them in water for 24 h. We did not pretreat F. angustifolia seeds because at the time of collection they had already overwintered in the crowns, and therefore they had naturally undergone cold stratification, which is considered to break dormancy in this species [55]. Petri dishes were randomly distributed in the germination chamber and rotated every 3-4 days to avoid position effects.…”

Section: Experimental Designmentioning

confidence: 99%

Potential Germination Success of Exotic and Native Trees Coexisting in Central Spain Riparian Forests

Cabra-Rivas

Castro‐Díez

2016

International Journal of Ecology

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We compared potential germination success (i.e., percentage of produced seeds that germinate under optimal conditions), the percentage of empty and insect-damaged seeds, germinability (Gmax), and time to germination (Tgerm) between the exoticsAilanthus altissima,Robinia pseudoacacia,andUlmus pumilaand two coexisting native trees (Fraxinus angustifoliaandUlmus minor) in the riparian forests of Central Spain. Additionally, we tested the effect of seed age, seed bank type (canopy or soil) and population onGmaxandTgermofA. altissimaandR. pseudoacacia, which are seed-banking species. Species ranked by their potential germination success wereA. altissima>U. pumila>R. pseudoacacia>U. minor>F. angustifolia. The combination of a highGmaxand negligible seed insect-damage providedA. altissimawith a potential germination advantage over the natives, which were the least successful due to an extremely high percentage of empty seeds or a very lowGmax.R. pseudoacaciashowed high vulnerability to insect seed predation which might be compensated with the maintenance of persistent seed banks with highGmax.GmaxandTgermwere strongly affected by seed age in the seed-banking invaders, but between-seed bank variation ofGmaxandTgermdid not show a consistent pattern across species and populations.

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The Effect of Different Temperatures and Durations on the Dormancy Breaking of Black Locust (Robinia pseudoacacia L.) and Honey Locust (Gleditsia triacanthos L.) Seeds

Cited by 6 publications

References 6 publications

Using automated sanding to homogeneously break seed dormancy in black locust (Robinia pseudoacaciaL., Fabaceae)

Using automated sanding to homogeneously break seed dormancy in black locust (Robinia pseudoacaciaL., Fabaceae)

Comparing the Sexual Reproductive Success of Two Exotic Trees Invading Spanish Riparian Forests vs. a Native Reference

Potential Germination Success of Exotic and Native Trees Coexisting in Central Spain Riparian Forests

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