The Effect of Different Levels of Cu, Zn and Mn Nanoparticles in Hen Turkey Diet on the Activity of Aminopeptidases

Abstract

The aim of the study was to estimate the influence of the different levels of Cu, Zn, and Mn nanoparticles on the activity of aminopeptidases in turkey. An experiment was carried out on 144 turkey hen Hybrid Converter. The birds were divided into groups given standard- and nanoparticle-supplementation of different level of copper (Cu 20, 10, 2 mg/kg), zinc (Zn 100, 50, 10 ppm), and manganese (Mn 100, 50, 10 ppm), covering respectively 100%, 50%, and 10% of the physiological demands for those minerals in the diet. The activity of aminopeptidases (alanyl: AlaAP, leucyl: LeuAP and arginyl: ArgAP) after supplementation of minerals was determined in the breast and thigh turkey muscle. The strongest effect of interaction among minerals supplementation form and dose on the activity levels of the aminopeptidases in thigh muscle was observed for nano-Cu already at the lowest dose of 2 mg/kg. In this dose (covering 10% of the birds’ demand) nano form of supplementation significantly increased the activity of Ala-, Leu-, and ArgAP (877, 201, and 719, respectively), compared to standard form of supplementation (461, 90.5, and 576, respectively). In turn, in breast muscle, after supplementation covering 10% of the demand with the nano-Cu, nano-Zn, and nano-Mn compared to the standard form, we did not observe any significant difference in the activity levels of any of the investigated aminopeptidases, except for AlaAP under Zn supplementation. Supplementation with the 20 mg/kg of Nano-Cu (100% of demand) and with 10 mg/kg of Nano-Cu (50% of demand) inhibited the activity of all of the three aminopeptidases in thigh muscle. Supplementation of the minerals in nano form into the diet, especially of Cu and Zn in the dose covering 10% of the demand is relevant to maintain homeostasis in turkey muscles, as indicated by the activity of the aminopeptidases.

The efficacy of metal nanocomposite (Fe3O4/CuO/ZnO) to ameliorate the toxic effects of ochratoxin in broilers

“…In the present study serum TP, albumin, globulin, Ca and Ph levels revealed that no significant differences in birds fed on MNc only (G3 & G4) and those of control group (G1), while significant decrease was recorded in G2 as shown in Tables 3 and 4 . OTA may integrate; inhibition of protein synthesis, disruption of cell signaling and division, impaired mitochondrial oxidation reactions and altered Ca homeostasis through suppressing the phosphoenolpyruvate carboxylase in the proximal renal tubules that alters the structural and functional renal ability to metabolize Ca [ 87 ] CuNP [ 73 ] enhances protein synthesis through utilized amino acids, improves aminopeptidases activity that is vital for protein digestion in muscles [ 74 ] and (Cu and Zn) NPs [ 39 ] have tendency to increase the content of serum protein and the activity of aminotransferases without any changes in liver microstructure. Meanwhile, low doses caused less increase in liver enzyme activities than high doses.…”

The effect of metal-containing nanoparticles on the health, performance and production of livestock animals and poultry

“…Supplementation of zinc in nano-form into the diet of turkey hens in the dose covering 10% of the demand (10 mg/kg of diet) maintained homeostasis in turkey muscles, as indicated by the activity of the aminopeptidases (alanyl, leucyl, and arginyl) (Jóźwik et al 2018 ).…”

The efficacy of metal nanocomposite (Fe 3 O 4 /CuO/ZnO) to ameliorate the toxic effects of ochratoxin in broilers

“…Also, Pu et al, [46] As in table (6) OTA residue in both liver and muscle, samples of G2 revealed a signi cant increase than all treated groups, while both doses of treatment showed no signi cant difference than G1. It was established that body weight signi cantly increased on feeding CuNP ( [52][53] which signi cantly improved aminopeptidases activity that is vital for protein digestion in muscles [54], ZnONP [55],Cu and Zn NP which resulted in a study [20] in a positive productive effect and muscle tissue by greater accompanied by increased bioavailability of the assessed microelements than inorganic form of mineral supplements. It is proved that feeding on Fe 3 O 4 NPs improved the nal weight and increased bioavailability of trace minerals which enhanced health [56][57][58] The studied haematological and immunological parameters revealed that birds had OTA feeding only (G2) showed signi cant reduction than G1 in TLC, heterophils and monocytes percentages.…”

“…In the present study blood TLC, albumin, globulin, Ca and Ph levels revealed that no signi cant differences in birds fed on MNc only (G3 & G4) and those of control group (G1), while signi cant decrease was recorded in G2 as shown in table (3). OTA may integrate; inhibition of protein synthesis, disruption of cell signaling and division, impaired mitochondrial oxidation reactions and altered Ca homeostasis [69] CuNP [52] enhances protein synthesis through utilized amino acids, improves aminopeptidases activity that is vital for protein digestion in muscles [55] and (Cu and Zn) NPs [20] have tendency to increase the content of serum protein and the activity of aminotransferases.…”