The effect of different extenders on the sperm motilityand viability of frozen Turkey semen

Kuzlu, Mehmet K.; Taşkın, Atilla

doi:10.18805/ijar.v0iof.6825

Cited by 11 publications

(12 citation statements)

References 12 publications

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“…In a similar study on ducks, as a result of freezing / thawing using 7% DMSO, the lowest vitality value was found as 21.75% and the highest as 32.45% (Setıoko et al, 2002). However, when the vitality and motility values found in studies using 5% DMSO and the same diluents (Kuzlu and Taskin, 2017) on turkeys and 5% DMSO on chickens were compared to the values found in this study, differences were observed. It is thought that these differences may have resulted from the chemical structure of DMSO, the change it made in the diluent used together, and the different stotoxic effect on different poultry spermatozoa (Sexton, 1982).…”

Section: Resultscontrasting

confidence: 65%

Effects of Extenders and Cryoprotectants on Cryopreservation of Duck Semen

Taşkın

Ergün

Karadavut

et al. 2020

Turkish JAF Sci.Tech.

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In this study, it was aimed to determine the usability of different diluents and cry protectants in cryopreservation of Peking duck semen. Semen samples were collected from five male ducks, twice a week for four weeks, using the abdominal massage method. In the study, 40 semen samples were used. Semen samples from ducks were combined (mixed) after being evaluated individually. Mix semen volume (ml), density (×109 / mL), pH, motility (%) and vitality (%) values were determined as 1.84±0.04, 1.72±0.8, 6.88±0.29, 71.55±3.28 and 74.27±4.67 respectively. In the study, as cry protectant, Glucose I (GⅠ) containing 6%DMF (dimethylformamide), Tris-Glucose I (TI), Lactated Ringer's-Glucose I (LGI), Lactated Ringer's I (LI) and Glucose II (GⅠI) containing 5% DMSO, Tris-Glucose II (TII), Lactated Ringer's-Glucose II (LGII), Lactated Ringer's II (LII) were used as diluents. The mixed semen was divided into eight equal parts and 1:3 ratio diluent was added and equilibrated in straws for 90 minutes at + 4°C. The straws were first frozen to -80°C with liquid nitrogen vapour, and then stored in liquid nitrogen at -196°C. After freezing and thawing, the highest motility value was determined in diluent LGI with 41.91%. In other diluents used, the mortality values were determined as LII (33.10%), LGII (32.80%), LI (29.00%), GI (23.66%), TI (12.91%), GII (11.22%) and TII (6.90%). Vitality (%) values were determined as LGI (44.08%), LII (38.50%), LGII (35.10%), LI (33.58), GI (30.91%), GII (23.10%), TI (20.66%) and TII (14.80%) respectively. In this study, it has been determined that the lactated Ringer's-Glucose diluent containing 6% DMF is more advantageous in terms of semen vitality and mortality values when compared to other diluents and that it can be used for long-term storage of duck sperm.

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Section: Resultscontrasting

confidence: 65%

Effects of Extenders and Cryoprotectants on Cryopreservation of Duck Semen

Taşkın

Ergün

Karadavut

et al. 2020

Turkish JAF Sci.Tech.

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“…It was also in the vas deferens where sperm produced by the testicles were stored until the rooster's companion (Salting et al, 2016). These differences may be explained by breed, location, nutrition, age and climate differences because the sperm parameters can vary depending on these factors (Das et al, 2016;Kuzlu and Taskin, 2017;Mavi et al, 2018). As presented in table 2, the percentage of morphologically abnormal spermatozoa in samples retrieved by float-out was 30.33±4.68 % while 30.33±4.68 % abnormality was observed in flushing retrieved samples too.…”

Section: Spermatozoa Retrieved Of the Vas Deferens By Float-out And Fmentioning

confidence: 98%

The characterization of Post-Mortem Sperm of Local Chicken Cocks in Eastern Algeria

Ouennes¹,

Bouzebda²,

Bouzebda³

et al. 2019

jwpr

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The present aimed to investigate for the first time the characteristics and conservation of post-mortem sperm, obtained from epididymis and the vas deferens of 18 pairs of adult local chicken cocks from the east of Algeria (age, 12-24 months, body weight 1.50-2.53 kg). And compare the sperm quality at the epididymis and the vas deferens levels, the efficacy of two post-mortem sperm retrieval techniques, the flushing and float-out methods in the collection of local chicken cock's sperm, and the effects of conservation in situ at different temperatures (2h and 24h at 20°C and after refrigeration at 4°C for 24h). The quality was significantly higher (0.13±0.05ml vs 0.72±0.12ml) in the vas deferens compared to the epididymis, whereas spermatozoa abnormalities and chromatin quality did not differ in both the epididymis and vas deferens. No significant difference was observed between the flushing and float-out methods. Concentration (3.33±1.63 million sperm vs 1.75±0.76 million sperm), initial motility (77.50±6.89% vs 75.83±8.61%), viability (75±10.39% vs 74.67±10.15%) and abnormality (30.33±4.68% vs 30.33±4.68%), only the volume was significantly higher (0.72±0.12ml vs 0.17±0.08ml). However, the effects of conservation in situ, at 25°C and 4°C for 24h, showed a significant difference for viability and motility of the spermatozoa recovered from vas deferens, and no significant difference for abnormality and acrosome integrity. Therefore, it can be concluded that good quality semen samples can be collected from the vas deferens with the flushing method, and semen of Algerian local cocks can be preserved at 20°C for 24h.

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“…The use of lactated ringer-glucose extender induces higher post-thawing turkey spermatozoa motility when compared to lactated ringer only (Kuzlu & Taskin 2017). According to the study of Mayesta, Trilaksana & Bebas (2014), motility of spermatozoa with 0.6% glucose treatment gave the best results to maintain the motility of Native Rooster spermatozoa on phosphate-egg yolk extenders stored at 3-5 o C. Based on this description the research conducted aims to find the best combination of glucose concentration with the type of extender (lactate ringer egg yolk, lactate ringer skim milk, coconut water egg yolk, and coconut water skim milk) to maintaining progressive motility and viability of Native rooster spermatozoa stored for 48 hours at 5 o C.…”

mentioning

confidence: 94%

The Effect of the Combination of Glucose Concentration with the Type of Extenders on the Quality of Native Rooster Spermatozoa during Storage

Khaeruddin¹,

Amir²

2019

Chalaza Journal of Animal Husbandry

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This study aimed to findthe best combination of glucose concentration with the type of extender to maintain progressive motility and viability of Native Rooster spermatozoa, which stored for 48 hours at 5oC. This study used a completely randomized design (CRD) factorial pattern of 4 x 3. The first treatment was a different type of extender: LE (lactated ringer egg yolk), LS (lactated ringer skim milk), CE (coconut water egg yolk) and CS (coconut water skim milk), while second treatment was glucose concentration (20mM, 50mM, and 80mM). Native Rooster semen diluted with a ratio of 1:10, thensperm was stored in the refrigerator (5oC). Progressive motility and viability were observed at 0, 24, 48, and 72 hours. The results showed the type of extender treatment had a very significant effect (P <0.01) on the viability of spermatozoa, which were stored 24-hour. The treatment of extender type, glucose concentration, and interaction of extender type with glucose concentration had a very significant effect (P <0.01) on the quality of spermatozoa, which were stored 48 hours and 72 hours. The highest progressive motility of spermatozoa was maintained up to 46.67 ± 2.89% and spermatozoa viability up to 74.68 ± 4.51% in WE 80mM glucose extenders, so the conclusion of this study is the combination of egg yolk coconut water with 80mM glucose is the best extender to maintain progressive motility and viability of spermatozoa of Native Rooster which is stored for 72 hours at 5oC.

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The effect of different extenders on the sperm motilityand viability of frozen Turkey semen

Cited by 11 publications

References 12 publications

Effects of Extenders and Cryoprotectants on Cryopreservation of Duck Semen

Effects of Extenders and Cryoprotectants on Cryopreservation of Duck Semen

The characterization of Post-Mortem Sperm of Local Chicken Cocks in Eastern Algeria

The Effect of the Combination of Glucose Concentration with the Type of Extenders on the Quality of Native Rooster Spermatozoa during Storage

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