The effect of dietary lipid manipulation on murine splenic lymphocytes apoptosis and heat shock protein over expression

Carratelli, Caterina Romano; Nuzzo, I.; Vitiello, T; Galdiero, Emilia; Galdiero, F

doi:10.1111/j.1574-695x.1999.tb01260.x

Cited by 14 publications

(6 citation statements)

References 24 publications

(20 reference statements)

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“…It is therefore feasible in our model that HFD feeding led to modified lipid composition of immune cells, resulting in impaired T‐cell signalling and polarization. Among lipids, some types of fatty acids have been shown to trigger apoptotic mechanisms [64]. Nevertheless, this effect is probably not involved in decreased T‐cell response, as a similar percentage of splenic T cells were found in HFD an SD animals.…”

Section: Discussionmentioning

confidence: 99%

Influence of High‐Fat Feeding on Both Naive and Antigen‐Experienced T‐Cell Immune Response in DO10.11 Mice

et al. 2006

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Obesity is becoming one of the most serious public health problems in industrialized societies, due to the profound changes in lifestyle, and notably in nutrition. Beside diabetes, cardiovascular diseases or hypertension, increased susceptibility to infection is one of the pathological consequences of being overweight. In this paper, we have assessed the influence of a high‐fat diet (HFD) rich in saturated fatty acids on the immune system of DO11.10 mice, which are transgenic for a T‐cell receptor specifically recognizing a peptide of ovalbumin. We showed that the specific T‐cell immune response was impaired by high‐fat feeding, and that the expression of this defect is different depending on whether T cells are naive or Ag experienced. Indeed, on in vitro ovalbumin stimulation, spleen T cells from naive HFD‐fed transgenic mice showed proliferation similar to that of cells from standard diet (SD)‐fed mice, but exhibited a strong inflammatory profile as shown by the markedly increased IFN‐γ/IL‐4 ratio. Inversely, spleen T cells from ovalbumin‐immunized HFD mice were impaired in their Ag‐dependent proliferation compared to cells from SD mice. By co‐culture experiments, we showed that both T cells and antigen‐presenting cells were involved in this impairment. Moreover, in ovalbumin‐immunized HFD animals, a trend towards Th2 response was noted, compared to immunized SD mice. This data implies that naive T cells could participate actively in the low‐grade systemic inflammation observed in overweight patients. Moreover, the impaired activity of Ag‐experienced T cells could have major consequences both in defence against infection and/or in vaccination protocols.

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Section: Discussionmentioning

confidence: 99%

Influence of High‐Fat Feeding on Both Naive and Antigen‐Experienced T‐Cell Immune Response in DO10.11 Mice

et al. 2006

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“…Recently, several reports have described the important role of fatty acids as apoptosis inducers or as substances capable of reducing cell death in cellular cultures 71 , 72 , 73 , 74 , 75 . It is very important to determine the role of free fatty acids in programmed cell death as a possible mechanism of action in the modulatory activity attributable to fatty acids.…”

Section: Discussionmentioning

confidence: 99%

“…In this case, the inhibition of cytosolic phospholipase A 2 could be involved in the inhibitory activity of this polyunsaturated fatty acid 74 . In addition to influence on in vitro cultures, dietary fatty acids may induce apoptosis, because a recent study has demonstrated that splenic cells from mice fed hyperlipidic diets containing saturated fatty acids were more susceptible to death by apoptosis than splenic cells from mice fed unsaturated fatty acids 75 . It is noteworthy that these findings may provide another mechanism by which fatty acids promote immune system modulation.…”

Section: In Vitro and In Vivo Studies Of Fatty Acids As Inducers Of Amentioning

confidence: 99%

Modulatory effects of dietary lipids on immune system functions

2000

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Summary Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis, natural killer (NK) cell activity, phagocytosis and so on. The immunomodulation induced by dietary fatty acids may be applied in the amelioration of inflammatory disorders, such as autoimmune diseases. However, the mechanisms that participate in these processes are still poorly understood. It is probable that modulation of immune system by fatty acids of the diet may occur by alteration of membrane fluidity, lipid peroxide formation, eicosanoid production or regulation of gene expression. However, recent studies have reported the effects of several free fatty acids on apoptosis induction of in vitro cultures. In fact, a possible explanation of the effects that fatty acids promote on the immune system cells could be associated with an apoptotic process performed in an irreversible way. In vivo studies have demonstrated the ability of fatty acids to alter the survival of animals fed diets containing oils and infected with a pathogenic bacterium. Experimental infection in animals fed dietary lipids produces a modification of resistance to micro-organisms. The present review analyses all of these parameters that dietary fatty acids are capable of altering in order to modify the immune response. Further studies will be needed to establish the mechanisms involved in immune system regulation, reduction of symptoms derived from autoimmune pathologies and so on.

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“…Therefore, we can hypothesize that the reduced ability of PBMC from TA cows to respond to lectins during the first 48–72 h fasting might be due to the fact that when isolated from blood, PBMC were in a distressed state due to a reduced glucose uptake. On the other hand, other studies demonstrated that fasting per se is associated with an increase of cell death by apoptosis (Pires et al 2006), and that saturated FA increase predisposition of lymphocytes to such event (Carratelli et al 1999). After 48–72 h fasting, increased availability of NEFA and ketones may have restored the ability of immune cells from TA cows to respond to lectins stimulation.…”

Section: Discussionmentioning

confidence: 97%

Effects of intravenous triacylglycerol emulsions on lymphocyte responses to mitogens in fasted dairy cows undergoing intense lipomobilization

Lacetera

Scalia

Mashek

et al. 2007

Journal of Dairy Research

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The objective of the study was to assess the effects of intravenous infusion of triacylglycerol (TAG) emulsions derived from different lipid sources on responses to mitogens of peripheral blood mononuclear cells (PBMC) isolated from fasted dairy cows. Six multiparous, non-pregnant, non-lactating Holstein cows were used in a replicated 3x3 Latin Square design. For 4 d, cows were fasted and infused intravenously with a 20% TAG emulsions derived from tallow (TA), linseed oil (LO) or fish oil (FO). Fasting was employed to induce energy deficit and lipid mobilization. Emulsions were administered for 20 to 30 min every 4 h throughout the 4 d fast at a rate of 0.54 g TAG/kg BW/d. Blood samples were taken before the first infusion, and then every 24 h during the fast. Blood was utilized to assess DNA synthesis, IgM and interferon-gamma (IFN-gamma) secretion by PBMC stimulated with mitogens. In TA infused cows there was a decline of PBMC ability to respond to mitogens, which was significant 48 h after initiation of the infusion period for DNA synthesis and IFN-gamma secretion. In LO or FO infused cows, PBMC responses to mitogens were not altered during the infusion period, and in some cases PBMC responses to mitogen was improved at 72 and 96 h after initiation of treatments. Effects of TAG infusion on PBMC responses to mitogens depended on the lipid source suggesting that LO or FO can attenuate the negative effects of fasting on immune functions.

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The effect of dietary lipid manipulation on murine splenic lymphocytes apoptosis and heat shock protein over expression

Cited by 14 publications

References 24 publications

Influence of High‐Fat Feeding on Both Naive and Antigen‐Experienced T‐Cell Immune Response in DO10.11 Mice

Influence of High‐Fat Feeding on Both Naive and Antigen‐Experienced T‐Cell Immune Response in DO10.11 Mice

Modulatory effects of dietary lipids on immune system functions

Effects of intravenous triacylglycerol emulsions on lymphocyte responses to mitogens in fasted dairy cows undergoing intense lipomobilization

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