The effect of dietary fatty acid composition on adipose tissue quality and expression of genes related to lipid metabolism in porcine livers

Świątkiewicz, Małgorzata; Oczkowicz, Maria; Ropka‐Molik, Katarzyna; Hańczakowska, E.

doi:10.1016/j.anifeedsci.2016.03.020

Cited by 16 publications

(26 citation statements)

References 38 publications

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“…In particular, 11 genes, including C-C motif chemokine 22 precursor ( CCL22 ), RNA-binding region containing protein 2-like ( S100A9 ), immune-responsive gene 1 protein homolog ( IRG1 ), cytochrome P450 2C49 precursor ( CYP2C49 ), DNA nucleotidylexotransferase-like ( DNTT ), putative aldo-keto reductase family 1 member C1 ( AKR1C1 ), inter-alpha-trypsin inhibitor heavy chain H4 precursor ( ITIH4 ), uteroglobin-like ( SCGB1A1 ), serum amyloid A2 precursor ( SAA4 ), serpin A3-8 ( ENSSSCG00000030371 ), and immune-responsive gene 1 protein homolog ( ENSSSCG00000030557 ), were differentially expressed among the three groups. The CYP2C49 gene is associated with lipid metabolism, as coconut oil and beef tallow increased its expression levels, while backfat subcutaneous adipose tissue showed increased SFAs and decreased MUFAs and PUFAs 26 . Our study showed that CYP2C49 was down-regulated in the L. reuteri group, which may be associated with the fatty acid composition in the intestine.…”

Section: Discussionmentioning

confidence: 99%

“…Swiatkiewicz et al . 26 reported that the presence of lipids in pig tissues and their degree of saturation affect the sensory quality of the meat, while adipose tissue metabolism depends on dietary fatty acid composition and is controlled by changes in gene expression. Therefore, we will conduct more experiments to elucidate the effect of Lactobacillus strains on the fatty acid composition of porcine muscle, which is critical for pork quality and human health.…”

Section: Discussionmentioning

confidence: 99%

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Gene expression profile changes in the jejunum of weaned piglets after oral administration of Lactobacillus or an antibiotic

Zhang

Shang

Huang

et al. 2017

Sci Rep

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The small intestine plays an essential role in the health and well-being of animals. Previous studies have shown that Lactobacillus has a protective effect on intestinal morphology, intestinal epithelium integrity and appropriate maturation of gut-associated tissues. Here, gene expression in jejunum tissue of weaned piglets was investigated by RNA-seq analysis after administration of sterile saline, Lactobacillus reuteri, or an antibiotic (chlortetracycline). In total, 401 and 293 genes were significantly regulated by chlortetracycline and L. reuteri, respectively, compared with control treatment. Notably, the HP, NOX1 and GPX2 genes were significantly up-regulated in the L. reuteri group compared with control, which is related to the antioxidant ability of this strain. In addition, the expression of genes related to arachidonic acid metabolism and linoleic acid metabolism enriched after treatment with L. reuteri. The fatty acid composition in the jejunum and colon was examined by GC-MS analysis and suggested that the MUFA C18:1n9c, and PUFAs C18:2n6c and C20:4n6 were increased in the L. reuteri group, verifying the GO enrichment and KEGG pathway analyses of the RNA-seq results. The results contribute to our understanding of the probiotic activity of this strain and its application in pig production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gene expression profile changes in the jejunum of weaned piglets after oral administration of Lactobacillus or an antibiotic

Zhang

Shang

Huang

et al. 2017

Sci Rep

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“…We observed that none of the dietary factors (cDDGS or fat) affected body weight or body composition. Contrary, the fatty acid composition of adipose tissue differed among dietary groups and was highly correlated with the fatty acid composition of diets [8]. Adipose tissue from animals consuming rapeseed oil contained significantly higher amounts of unsaturated fatty acids [7].…”

Section: Introductionmentioning

confidence: 90%

“…In this manuscript, we describe the analysis of the effect of source of dietary fat on gene expression in adipose tissue of pigs, based on part of the data obtained in a previously described RNA sequencing experiment [7]. All procedures describing animal housing are described elsewhere [8]. In brief, crossbred fatteners were kept in individual straw-bedded pens in uniform conditions.…”

Section: Animals and Dietsmentioning

confidence: 99%

Source of Dietary Fat in Pig Diet Affects Adipose Expression of Genes Related to Cancer, Cardiovascular, and Neurodegenerative Diseases

Oczkowicz

Szmatoła

Świątkiewicz

2019

Genes

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It has been known for many years that excessive consumption of saturated fats has proatherogenic properties, contrary to unsaturated fats. However, the molecular mechanism covering these effects is not fully understood. In this paper, we aimed to identify differentially expressed genes (DEGs) using RNA-sequencing, following feeding pigs with different sources of fat. After comparison of adipose samples from three dietary groups (rapeseed oil (n = 6), beef tallow (n = 5), coconut oil (n = 5)), we identified 29 DEGs (adjusted p-value < 0.05, fold change > 1.3) between beef tallow and rapeseed oil and 2 genes between coconut oil and rapeseed oil groups. No differentially expressed genes were observed between coconut oil and beef tallow groups. Almost all 29 DEGs between rapeseed oil and beef tallow groups are connected to neurodegenerative, cardiovascular diseases, or cancer (e.g., PLAU, CYBB, NCF2, ZNF217, CHAC1, CTCFL). Functional analysis of these genes revealed that they are associated with fluid shear stress response, complement and coagulation cascade, ROS signaling, neurogenesis, and regulation of protein binding and protein catabolic processes. Furthermore, gene set enrichment analysis (GSEA) of the whole datasets from all three comparisons suggests that both beef tallow and coconut oil may trigger changes in the expression level of genes crucial in the pathogenesis of civilization diseases.

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“…edu.tw/php/index.php) -a database that contains information regarding experimentally proven miRNA-gene interactions -was scanned to find putative miRNAs that may regulate expression of the differentially expressed genes that we pre- maize DDGS -maize dried distilled grain with solubles; SFA -saturated fatty acids; UFA -unsaturated fatty acids; MUFA -monounsaturated fatty acids; PUFA -polyunsaturated fatty acids viously identified in a RNA-Seq experiment Świątkiewicz et al, 2016). The 61 distinct miRNAs were identified.…”

Section: Selection Of Mirnas By In Silico Mirtarbase Analysismentioning

confidence: 99%

Addition of coconut oil to the diet based on maize dried distilled grains with solubles (DDGS) alters miR-122a expression in the pig liver

Oczkowicz¹,

Pawlina²,

Bugno‐Poniewierska³

et al. 2017

J. Anim. Feed Sci.

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The effect of dietary fatty acid composition on adipose tissue quality and expression of genes related to lipid metabolism in porcine livers

Cited by 16 publications

References 38 publications

Gene expression profile changes in the jejunum of weaned piglets after oral administration of Lactobacillus or an antibiotic

Gene expression profile changes in the jejunum of weaned piglets after oral administration of Lactobacillus or an antibiotic

Source of Dietary Fat in Pig Diet Affects Adipose Expression of Genes Related to Cancer, Cardiovascular, and Neurodegenerative Diseases

Addition of coconut oil to the diet based on maize dried distilled grains with solubles (DDGS) alters miR-122a expression in the pig liver

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