The effect of diabetes on the wound healing potential of adipose‐tissue derived stem cells

Kim, Sue Min; Kim, Yun Ho; Jun, Young Joon; Yoo, Gyeol; Rhie, Jong Won

doi:10.1111/iwj.12540

Cited by 30 publications

(39 citation statements)

References 17 publications

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“…Additionally, glucose and amino acid entry into cells and the acceleration of protein synthesis, with increased fat synthesis and steatolysis inhibition promote wound granulation (6). Furthermore, the increase in the expression of epidermal growth factor and basic fibroblast growth factor in wound tissues promotes fibroblast proliferation, increasing the hydroxyproline content in wound tissue, advancing the epidermal cell proliferation cycle and increasing synthesis of collagen fibers in the wounded tissue (7). The initial stage of repair and the proliferation of apoptosis after deep burns has the following adverse effects: Growth factor expression is weakened, granulation of the wounded tissues is not normal and consequently the survival rate of free flaps is not high due to the lack of a nurturing environment.…”

Section: Discussionmentioning

confidence: 99%

Clinical study on local application of low-dose insulin for promoting wound healing after operation for deep burns

Zeng

Zhi

Liu

et al. 2016

Experimental and Therapeutic Medicine

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Transplanted free skin flaps are often needed to treat deep burns; their survival, however, is less than optimal. This study examined whether local low-dose insulin injections can promote flap survival and wound healing after surgery. A total of 165 patients who underwent free skin flap transplantation for simple deep burns were enrolled in the study and divided into 5 groups of 33 patients each: Blank control group (no local subcutaneous drug injections), saline control group (saline injections), low-dose insulin group (0.5 units regular insulin injections), medium-dose group (1.0 units regular insulin injections) and high-dose group (2.0 units regular insulin injections). Wound healing and flap survival conditions were assessed and compared among groups. The best wound healing rate found was that of the low-dose insulin injection group where all the parameters measured improved significantly: The healing time was shorter; the blood flow volume, the flap survival, the number of fibroblasts and new vessels increased; the re-epithelialization occurred faster; the infiltration of inflammatory cells was reduced; the expression levels of heat shock protein-90, vascular endothelial growth factor, transforming growth factor-β and interleukin-1 were higher; and the plasma glucose levels only fluctuated slightly. The results clearly demonstrate that a local low-dose insulin regime after flap transplantation can accelerate the healing time and improve the surgical outcome without exerting detrimental secondary effects on the glucose plasma level of deep burn patients.

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Section: Discussionmentioning

confidence: 99%

Clinical study on local application of low-dose insulin for promoting wound healing after operation for deep burns

Zeng

Zhi

Liu

et al. 2016

Experimental and Therapeutic Medicine

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“…Kim and colleagues [107] performed a study comparing normal ASCs to diabetic ASCs in a wound-healing model created by including a silicone layer in the artificial dermis in order to minimize skin contraction around the wound site. At 1 week, the wound-healing rate was significantly higher in the normal ASC group than in the diabetic ASC group and the untreated control group.…”

Section: Application Of Ascs For Chronic Wound Healing Inmentioning

confidence: 99%

The Role of Adipose-Derived Stem Cells, Dermal Regenerative Templates, and Platelet-Rich Plasma in Tissue Engineering-Based Treatments of Chronic Skin Wounds

Conese

Annacontini

Carbone

et al. 2020

Stem Cells International

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The continuous improvements in the field of both regenerative medicine and tissue engineering have allowed the design of new and more efficacious strategies for the treatment of chronic or hard-to-heal skin wounds, which represent heavy burden, from a medical and economic point of view. These novel approaches are based on the usage of three key methodologies: stem cells, growth factors, and biomimetic scaffolds. These days, the adipose tissue can be considered the main source of multipotent mesenchymal stem cells, especially adipose-derived stem cells (ASCs). ASCs are easily accessible from various fat depots and show an intrinsic plasticity in giving rise to cell types involved in wound healing and angiogenesis. ASCs can be found in fat grafts, historically used in the treatment of chronic wounds, and have been evaluated as such in both animal models and human trials, to exploit their capability of accelerating wound closure and inducing a correct remodeling of the newly formed fibrovascular tissue. Since survival and fitness of ASCs need to be improved, they are now employed in conjunction with advanced wound dressings, together with dermal regenerative templates and platelet-rich plasma (as a source of growth and healing factors). In this work, we provide an overview of the current knowledge on the topic, based on existing studies and on our own experience.

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“…First, the aberrant in vivo micro milieu of people with DM adversely affects the therapeutic biological action of the ADSs on wound repair 20. Second, the systemic deficiency of the healing potential of diabetic ADS in vivo 21. Third, ADS is physiologically damaged 22.…”

Section: Introductionmentioning

confidence: 99%

Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus

Ebrahimpour-Malekshah

Amini

Zare

et al. 2020

BMJ Open Diab Res Care

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ObjectiveWe assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1).Research design and methodsWe randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm2, energy density: 0.2 J/cm2) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 received both the human ADS and PBM. In addition, we inflicted an ischemic, delayed healing, and infected wound simulation in all of the rats. The wounds were infected with methicillin-resistant Staphylococcus aureus (MRSA).ResultsAll three treatment regimens significantly decreased the amount of microbial flora, significantly increased wound strength and significantly modulated inflammatory response and significantly increased angiogenesis on day 16. Microbiological analysis showed that PBM+ADS was significantly better than PBM and ADS alone. In terms of wound closure rate and angiogenesis, PBM+ADS was significantly better than the PBM, ADS and control groups.ConclusionsCombination therapy of PBM+ADS is more effective that either PBM or ADS in stimulating skin injury repair, and modulating inflammatory response in an MRSA-infected wound model of rats with DM1.

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The effect of diabetes on the wound healing potential of adipose‐tissue derived stem cells

Cited by 30 publications

References 17 publications

Clinical study on local application of low-dose insulin for promoting wound healing after operation for deep burns

Clinical study on local application of low-dose insulin for promoting wound healing after operation for deep burns

The Role of Adipose-Derived Stem Cells, Dermal Regenerative Templates, and Platelet-Rich Plasma in Tissue Engineering-Based Treatments of Chronic Skin Wounds

Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus

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