The effect of deep pectoral myopathy on the properties of broiler chicken muscles characterised by selected instrumental techniques

Abstract

Changes in the biophysical traits of the pectoral muscles of chickens with deep pectoral myopathy (DPM) were analysed with selected instrumental techniques. For comparative purposes, the research used both samples of pectoralis minor muscles of 39-42-day-old Cobb 500 broiler chickens and pectoralis major muscles with DPM symptoms, as well. Computer Image Analysis (CIA) revealed that the pectoral minor muscles with DPM were characterised by smaller area of muscle fibres' cross-sectional area (CSA) than normal muscles. A longitudinal section of DPM muscles also confirmed the presence of large spaces between the bundles of muscle fibres. The results of Differential Scanning Calorimetry (DSC) analysis showed that all the temperatures of transformations referring to minor muscles with myopathy symptoms were significantly lower than the temperatures noted in the muscles without DPM. The enthalpy values observed in both pectoral muscles with DPM were significantly lower than the values noted in healthy muscles. The water dynamics analysed by low-field NMR showed that the values of spin-lattice relaxation time T 1 in p. major muscles without DPM were higher than the values in the muscles with the defect. On the other hand, an inverse dependence was observed in p. minor muscles samples. The value of the long relaxation time T 22 was reduced in both muscles with pathological lesions. In conclusion, three advanced analytical tools used in this study (CIA, DSC, and LF NMR) provide new insight into the characterization of protein changes observed in the pectoral muscles of chicken broilers with DPM.

Impact of deep pectoral myopathy on chemical composition and quality parameters of chicken breast fillet

“…The lower protein content found in the meat samples affected by DPM score 2 may indicate muscle fiber degeneration and further studies are needed to note that the presence of myopathies causes changes in the integrity of the muscle fiber and how this process occurs in DPM. Stangierski et al (2019) characterized deep pectoral myopathy in breast fillets and, reported unfavorable changes in the histological structure of fillet muscle fibers and the native state of proteins. Especially myosin, may have caused the redistribution of water, in limited migration of free water molecules, which may have been caused by structural changes in proteins.…”

Quality changes of duck meat during thermal sterilization processing caused by microwave, stepwise retort, and general retort heating

“…T 2 represented the degree of binding between water and the matrix as well as the fluidity of water ( 29 ). The higher the T 2 value, the looser the bond between water and the matrix and the greater the fluidity ( 30 ).…”

Deep Pectoral Myopathy in Broiler Chicken Obtained From Supermarket - Case Report, Literature Review and Preventive Measures

“…Distinctive swollen reddish-brown lesions appear fi rst and they are followed by lesion changes in color to green. Eventually, the lesions become pale green (Stangierski et al, 2019). Earlier fi ndings showed that pathological changes affect both major and minor pectoral muscles (Pastuszczak et al, 2002).…”