The effect of decellularized bone/bone marrow produced by high-hydrostatic pressurization on the osteogenic differentiation of mesenchymal stem cells

Hashimoto, Yoshihide; Funamoto, Seiichi; Kimura, Tsuyoshi; Nam, Kwangwoo; Fujisato, Toshiya; Kishida, Akio

doi:10.1016/j.biomaterials.2011.06.008

Cited by 102 publications

(78 citation statements)

References 41 publications

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“…Decellularized matrices have been reported for scaffolding in many vital organs such as the kidney [19], [20], heart [21], [22], blood vessel [23], [24], and bone [25], [26]. In decellularization, the tissue of interest is subjected to extreme conditions such as high acidity, alkalinity or pressure.…”

Section: Introductionmentioning

confidence: 99%

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

et al. 2014

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Infertility caused by ovarian or tubal problems can be treated using In Vitro Fertilization and Embryo Transfer (IVF-ET); however, this is not possible for women with uterine loss and malformations that require uterine reconstruction for the treatment of their infertility. In this study, we are the first to report the usefulness of decellularized matrices as a scaffold for uterine reconstruction. Uterine tissues were extracted from Sprague Dawley (SD) rats and decellularized using either sodium dodecyl sulfate (SDS) or high hydrostatic pressure (HHP) at optimized conditions. Histological staining and quantitative analysis showed that both SDS and HHP methods effectively removed cells from the tissues with, specifically, a significant reduction of DNA contents for HHP constructs. HHP constructs highly retained the collagen content, the main component of extracellular matrices in uterine tissue, compared to SDS constructs and had similar content levels of collagen to the native tissue. The mechanical strength of the HHP constructs was similar to that of the native tissue, while that of the SDS constructs was significantly elevated. Transmission electron microscopy (TEM) revealed no apparent denaturation of collagen fibers in the HHP constructs compared to the SDS constructs. Transplantation of the decellularized tissues into rat uteri revealed the successful regeneration of the uterine tissues with a 3-layer structure 30 days after the transplantation. Moreover, a lot of epithelial gland tissue and Ki67 positive cells were detected. Immunohistochemical analyses showed that the regenerated tissues have a normal response to ovarian hormone for pregnancy. The subsequent pregnancy test after 30 days transplantation revealed successful pregnancy for both the SDS and HHP groups. These findings indicate that the decellularized matrix from the uterine tissue can be a potential scaffold for uterine regeneration.

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Section: Introductionmentioning

confidence: 99%

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

et al. 2014

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“…IPM is the matrix surrounding the outer segments of photoreceptors and is naturally acellular upon collection as described above (as noted by nuclear staining, data not shown). However, in contrast to decellularized bone, 35 blood vessels, 28 trachea, 29 heart, 30 tooth bud, 36 or lacrimal gland, IPM does not possess sufficient mechanical integrity to be easily handled, although we were able to collect it as a suspension. It is possible to use these suspended IPM particles to coat different surfaces by centrifugation, although they tend to detach from the surface, limiting experimental studies.…”

Section: Discussionmentioning

confidence: 99%

Interphotoreceptor matrix-poly(ϵ-caprolactone) composite scaffolds for human photoreceptor differentiation

et al. 2014

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Tissue engineering has been widely applied in different areas of regenerative medicine, including retinal regeneration. Typically, artificial biopolymers require additional surface modification (e.g. with arginine–glycine–aspartate-containing peptides or adsorption of protein, such as fibronectin), before cell seeding. Here, we describe an alternative approach for scaffold design: the manufacture of hybrid interphotoreceptor matrix-poly (ϵ-caprolactone) scaffolds, in which the insoluble extracellular matrix of the retina is incorporated into a biodegradable polymer well suited for transplantation. The incorporation of interphotoreceptor matrix did not change the topography of polycaprolactone film, although it led to a slight increase in hydrophilic properties (water contact angle measurements). This hybrid scaffold provided sufficient stimuli for human retinal progenitor cell adhesion and inhibited proliferation, leading to differentiation toward photoreceptor cells (expression of Crx, Nrl, rhodopsin, ROM1). This scaffold may be used for transplantation of retinal progenitor cells and their progeny to treat retinal degenerative disorders.

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“…We also showed the activity remained in nevus specimen in a WST-8 assay after ultrahigh pressurization at 1,000 MPa . HHP at pressures higher than 600 MPa has been reported to inactivate cells and most of the pathogens that are present in various tissues, including heart valves, blood vessels, corneas and bone/bone marrow [Hashimoto et al, 2010[Hashimoto et al, , 2011Mahara et al, 2014Mahara et al, , 2015. It is reported that HHP at pressures higher than 300 MPa causes irreversible protein denaturation and induces cell death through a necrotic-like pathway [Diehl et al, 2008;Rivalain et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

“…various decellularized tissues without damaging the native extracellular matrix [Hashimoto et al, 2010[Hashimoto et al, , 2011Mahara et al, 2015]. We previously reported that HHP at 200 MPa for 10 min can inactivate mammalian cells and that it is capable of inactivating skin and melanocytic nevus tissue in light brown to black patches or plaques that contain nevus cells [Mahara et al, 2014;Jinno et al, 2015;Liem et al, 2015;Morimoto et al, 2015].…”

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confidence: 99%

Verification of the Inactivation of Melanocytic Nevus in vitro Using a Newly Developed Portable High Hydrostatic Pressure Device

Morimoto

Jinno

Mahara

et al. 2016

Cells Tissues Organs

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High hydrostatic pressure (HHP) technology is a physical method for inactivating tissue. We reported that nevus specimens were inactivated after HHP at 200 MPa and that the inactivated nevus could be used as autologous dermis for covering skin defects. In this study, we verified the inactivation of nevus specimens using a newly developed portable HHP device which will be used in a clinical trial. Nevus tissue specimens were obtained from 5 patients (mean age 7.2 years, range 1-19). We cultured fibroblasts and nevus cells from the tissue specimens and then evaluated their inactivation after HHP at 200 MPa by confirming the attachment of the suspensions and by the live/dead staining of the suspensions, through the dissociation of the cells on chamber slides and by the live/dead staining of the remaining cells. The cells were also quantitatively evaluated by WST-8 assay. We then confirmed the inactivation of the nevus specimens after HHP using explant culture.Our results indicated that fibroblasts and nevus cells were inactivated after HHP at 200 MPa, with the exception of a small percentage of green-colored cells, which reflected the remaining activity of the cellular esterases after HHP. No cells migrated from the nevus specimens after HHP at 200 MPa. We verified the inactivation of fibroblasts and nevus cells cultured from nevus specimens, and in the nevus samples themselves after pressurization at 200 MPa using this device. This device could be used in clinical trials for giant congenital melanocytic nevi and may thus become useful in various medical fields.

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The effect of decellularized bone/bone marrow produced by high-hydrostatic pressurization on the osteogenic differentiation of mesenchymal stem cells

Cited by 102 publications

References 41 publications

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

Interphotoreceptor matrix-poly(ϵ-caprolactone) composite scaffolds for human photoreceptor differentiation

Verification of the Inactivation of Melanocytic Nevus in vitro Using a Newly Developed Portable High Hydrostatic Pressure Device

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