The Effect of Culture Media on Antigenic Expression in Sulfate-Reducing Bacteria

Cloete, T.E.; Bruyn, E.E. De

doi:10.1007/s002840010221

Cited by 5 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is unknown to what extent overexpressed proteins are associated with differences in virulence between bacterial biotypes. However, SDS‐PAGE gels must be treated with caution owing to variation caused by culture conditions (Cloete and De Bruyn 2001).…”

Section: Discussionmentioning

confidence: 99%

“…It is unknown to what extent overexpressed proteins are associated with differences in virulence between bacterial biotypes. However, SDS-PAGE gels must be treated with caution owing to variation caused by culture conditions (Cloete and De Bruyn 2001). The analysis of LPS showed that Y. ruckeri possesses a smooth LPS structure with long repeating polysaccharide O side chains, which is similar to other members of the Enterobacteriaceae (Perez-Perez et al 1986).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antigenic and cross-protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss (Walbaum)

Tinsley

Lyndon

Austin

2011

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: The study investigated antigen characteristics of biotype (bt) 1 and bt 2 isolates of Yersinia ruckeri. Methods and Results: The cell surface characteristics of Y. ruckeri were compared for their antigenic characteristics using polyclonal antibodies that revealed that both biotypes had a homogenous whole‐cell protein antigenic profile. Notable differences in the antigenic properties were observed in the lipopolysaccharide profile of both biotypes. Two iron‐regulated outer membrane proteins (IROMP) of c. 90 and 100 kDa were shown to be major specific antigens. The results demonstrate for the first time differences in antigens between bt 1 and bt 2 isolates of serotype O1 isolates of Y. ruckeri. The protection induced in rainbow trout by a commercial monovalent, and bivalent inactivated vaccine was tested with the outcome that the ability of isolates to cause mortality in vaccinated fish varied with geographical location. In this context, vaccination studies suggested that the O antigen was the dominant immunogenic molecule involved in protection against the disease. Conclusions: The O antigen of Y. ruckeri was the dominant immunogenic molecule involved in the protection of rainbow trout against enteric redmouth disease. Significance and Impact of the Study: There are distinct phenotypic and antigenic differences in Y. ruckeri bt 1 and bt 2 with O antigen recognized as the dominant immunogenic molecule. The data have significance in explaining the lack of success of the earlier monovalent vaccine and demonstrate the effectiveness of the newer bivalent vaccine.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antigenic and cross-protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss (Walbaum)

Tinsley

Lyndon

Austin

2011

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“… 101 Similarly, a number of other microbes have been reported to differentially express immunogenic molecules, depending on the growth medium. 103 – 107 Ft grown in vitro in Mueller Hinton Broth (MHB) expresses a distinct set of genes as compared to those obtained from tissues or MØs following Ft infection. 108 In addition, MHB-grown Ft ( Ft -MHB) can induce production of select proinflammatory cytokines, while Ft obtained from Ft -infected animals or MØs exhibit a reduced ability to do so.…”

Section: Major Factors Influencing Ft Vaccine Effimentioning

confidence: 99%

Tularemia vaccine development: paralysis or progress?

Sunagar

Kumar

Franz

et al. 2016

VDT

View full text Add to dashboard Cite

Francisella tularensis (Ft) is a gram-negative intercellular pathogen and category A biothreat agent. However, despite 15 years of strong government investment and intense research focused on the development of a US Food and Drug Administration-approved vaccine against Ft, the primary goal remains elusive. This article reviews research efforts focused on developing an Ft vaccine, as well as a number of important factors, some only recently recognized as such, which can significantly impact the development and evaluation of Ft vaccine efficacy. Finally, an assessment is provided as to whether a US Food and Drug Administration-approved Ft vaccine is likely to be forthcoming and the potential means by which this might be achieved.

show abstract

“…Specifically, the in vitro culture medium utilized to generate whole cell-based vaccines can impact the expression of antigenic determinants, pathogen-associated molecular patterns, and virulence factors expressed by the infectious agent. For example, many microbes have been reported to differentially express immunogenic molecules dependent on growth medium ( 12 – 16 ). Specifically, in a study of whole cell-based vaccines involving BCG, differential cultivation of BCG in Sauton versus Middlebrook medium significantly altered its protective efficacy and the enhanced protection generated by Middlebrook-derived BCG was associated with higher numbers of Mycobacteria-specific T helper type 17 (TH17) cells and higher Ab levels ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

Differential In Vitro Cultivation of Francisella tularensis Influences Live Vaccine Protective Efficacy by Altering the Immune Response

et al. 2018

View full text Add to dashboard Cite

Francisella tularensis (Ft) is a biothreat agent for which there is no FDA-approved human vaccine. Currently, there are substantial efforts underway to develop both vaccines and improved tools to assess these vaccines. Ft expresses distinct sets of antigens (Ags) in vivo as compared to those expressed in vitro. Importantly, Ft grown in brain-heart infusion medium (BHIM) more closely mimics the antigenic profile of macrophage-grown Ft when compared to Mueller-Hinton medium (MHM)-grown Ft. Thus, we predicted that when used as a live vaccine BHIM-grown Ft (BHIM-Ft) would provide better protection, as compared to MHM-Ft. We first determined if there was a difference in growth kinetics between BHIM and MHM-Ft. We found that BHIM-Ft exhibited an initial growth advantage ex vivo that manifests as slightly hastened intracellular replication as compared to MHM-Ft. We also observed that BHIM-Ft exhibited an initial growth advantage in vivo represented by rapid bacterial expansion and systemic dissemination associated with a slightly shorter mean survival time of naive animals. Next, using two distinct strains of Ft LVS (WT and sodB), we observed that mice vaccinated with live BHIM-Ft LVS exhibited significantly better protection against Ft SchuS4 respiratory challenge compared to MHM-Ft-immunized mice. This enhanced protection correlated with lower bacterial burden, reduced tissue inflammation, and reduced pro-inflammatory cytokine production late in infection. Splenocytes from BHIM-Ft sodB-immunized mice contained more CD4+, effector, memory T-cells, and were more effective at limiting intracellular replication of Ft LVS in vitro. Concurrent with enhanced killing of Ft LVS, BHIM-Ft sodB-immune splenocytes produced significantly higher levels of IFN-γ and IL-17A cytokines than their MHM-Ft sodB-immunized counterparts indicating development of a more effective T cell memory response when immunizing mice with BHIM-Ft.

show abstract

The Effect of Culture Media on Antigenic Expression in Sulfate-Reducing Bacteria

Cited by 5 publications

References 37 publications

Antigenic and cross-protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss (Walbaum)

Antigenic and cross-protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss (Walbaum)

Tularemia vaccine development: paralysis or progress?

Differential In Vitro Cultivation of Francisella tularensis Influences Live Vaccine Protective Efficacy by Altering the Immune Response

Contact Info

Product

Resources

About