The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum

Join the green team: Inducers of plant immunity in the plant disease sustainable control toolbox

“…Moreover, cryptogein secreted by Phytophthora cryptogea could induce an intense defense response in tobacco plants consisting of HR and SAR [80] . In addition, cryptogein also induces multiple signal transduction events in plants, including ROS and NO production, MAPK activation, cell death, lipid peroxidation, and LOX gene transcription [81] . A list of plant diseases managed by applying immune-inducing proteins in various plant species are summarized in Table 2 .…”

“…tomato DC3000 , Tobacco mosaic virus Leaf injection Hypersensitive response (HR), hydrogen peroxide production, extracellular-medium alkalization, callose deposition, phenolics metabolism, and lignin synthesis, cause necrotic lesions, induce SAR; trigger innate immunity and to result in the up-regulation of pathogen-related genes, metabolite deposition, cell wall modifications, JA signaling and Ca 2+ -responsive pathways PEL1 [75] Verticillium dahlia Tobacco, Cotton Botrytis cinerea, Verticillium dahlia Leaf syringe-infiltration Cell death, accumulation of ROS, defense-related genes expression, callose deposition Hrip1 [77] , [83] Magnaporthe oryzae, Alternaria tenuissima Rice, Tobacco Rice blast, Tobacco mosaic disease Magnaporthe oryzae, Tobacco mosaic virus Spray, Leaf infiltration Hydrogen peroxide production, callose deposition, alkalization of the extracellular medium, activation of SA signaling pathway, the gibberellin (GA) pathway, promote plant growth, enhance systemic resistance, hypersensitive response, NO production, SAR pathway, defense-related genes expression HpaXpm [84] Xanthomonas phaseoli pv. manihotis HNHK Tobacco Tobacco mosaic disease Tobacco mosaic virus Leaf injection, Foliar spray Hypersensitive response, NPR1 gene expression, promote plant growth PopW [85] Ralstonia solanacearum Tobacco Tobacco mosaic disease Tobacco mosaic virus Spray Hypersensitive response, SAR, induce expression of PR genes, H 2 O 2 burst, activity of defense-related enzymes, increase tobacco yield, improve tobacco foliar quality ELR [79] Solanum microdontum Potato Potato late blight Phytophthora infestans, oomycete plant pathogens Transgenic Cryptogein [78] , [81] Phytophthora cryptogea Tobacco Phytophthora parasitica Leaf infiltration Hypersensitive response, SAR, ROS and NO production, mitogen-activated protein kinase (MAPK) activation, cel...…”

Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions

“…The M. oryzae genome harbors 19 proteins with the CFEM domain (Kou et al, 2017). Two CFEM-containing proteins Wang et al, 2003;Zhang et al, 2004; Billard et al, 1988;Huet and Pernollet, 1989 P. colocasiae † 15-kDa glycoprotein Elicitin ND Induces cell death and SAR in taro leaf Mishra et al, 2009 P. cryptogea † Cryptogein Elicitin ND Induces cell death, SAR and defense of tobacco against P. nicotianae Ricci et al, 1989;Galiana et al, 1997;Mikes et al, 1997;Leborgne-Castel et al, 2008;Coursol et al, 2015;Kulik et al, 2015;Ptáčková et al, 2015;Starý et al, Huet et al, 1994;Kamoun et al, 1997Kamoun et al, , 1998Kanzaki et al, 2003;Huitema et al, 2005;Du et al, 2015 (Continued) Frontiers in Genetics | www.frontiersin.org Mateos et al, 1997;Khatib et al, 2004 P. parasitica † PpNLP/NLP Pp NLP ND Induces cell death; carries a nlp20 pattern recognized by RLP23 which associates with SOBIR1 and BAK1 complex to trigger immune responses Fellbrich et al, 2002;Qutob et al, 2006;Ottmann et al, 2009;Böhm et al, Kamoun et al, 1993;Mouton-Perronnet et al, 1995;Capasso et al, 1999 PPTG_02039, PPTG_14297, PPTG_09966 MoCDIP2 and MoCDIP11 have been identified to induce cell in the non-host N. benthamiana and host rice cells Guo et al, 2019). MoCDIP2 contains a CFEM domain at the N-terminus, and a GPI-anchored site at the C-terminus, whereas MoCDIP11 contains only a CFEM domain at the N-terminus.…”

“…However, the lipid-binding ability did not influence Pazzagli et al, 1999;Jeong et al, 2007;Frías et al, 2011 Elicitin ND + − Cryptogein, INF1 ELR BAK1, HSP70, HSP90, NbLRK1, SGT1, SRC2-1 Ricci et al, 1989;Huet et al, 1994;Kamoun et al, 1997;Kanzaki et al, 2008;Du et al, 2015; elicitin-induced response in tobacco. Investigations based on the mutant proteins of cryptogein with limited abilities to bind sterols revealed that induction of ROS synthesis, cytosol acidification and cell death in tobacco cells were not correlated with the sterolbinding abilities of the cryptogein proteins (Dokládal et al, 2012;Ptáčková et al, 2015).…”

“…However, the lipid-binding ability did not influence elicitin-induced response in tobacco. Investigations based on the mutant proteins of cryptogein with limited abilities to bind sterols revealed that induction of ROS synthesis, cytosol acidification and cell death in tobacco cells were not correlated with the sterol-binding abilities of the cryptogein proteins ( Dokládal et al, 2012 ; Ptáčková et al, 2015 ).…”