The effect of cryopreservation on anti-cancer activity of human amniotic membrane

Modaresifar, Khashayar; Azizian, Sara; Zolghadr, Maryam; Moravvej, Hamideh; Ahmadiani, Abolhassan; Niknejad, Hassan

doi:10.1016/j.cryobiol.2016.12.001

Cited by 29 publications

(21 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in results, exposing the AM to IL-1β resulted in a higher amount of elafin, HBD-2, and HBD-3 and cathelicidin LL-37 secretion which confirmed secretory state of amniotic antibacterial ingredients. This result is consistent with previous study in which we showed that AM releases some anti-cancer and immune-modulatory agents in condition medium 35 , 36 . Therefore, the non-secretory state of amniotic antibacterial ingredients is not acceptable and it seems that the disk diffusion method does not provide an appropriate condition for amnion to display its antibacterial property.…”

Section: Discussionsupporting

confidence: 94%

Induction of antimicrobial peptides secretion by IL-1β enhances human amniotic membrane for regenerative medicine

Tehrani

Modaresifar

Azizian

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

Due to antibacterial characteristic, amnion has been frequently used in different clinical situations. Developing an in vitro method to augment endogenous antibacterial ingredient of amniotic epithelial and mesenchymal stem cells is desirable for a higher efficacy of this promising biomaterial. In this study, epithelial or mesenchymal side dependent effect of amniotic membrane (AM) on antibacterial activity against some laboratory and clinical isolated strains was investigated by modified disk diffusion method and colony count assay. The effect of exposure to IL-1β in production and release of antibacterial ingredients was investigated by ELISA assay. The results showed that there is no significant difference between epithelial and mesenchymal sides of amnion in inhibition of bacterial growth. Although the results of disk diffusion showed that the AM inhibitory effect depends on bacterial genus and strain, colony count assay showed that the extract of AM inhibits all investigated bacterial strains. The exposure of AM to IL-1β leads to a higher level of antibacterial peptides secretion including elafin, HBD-2, HBD-3 and cathelicidic LL-37. Based on these results, amniotic cells possess antibacterial activity which can be augmented by inflammatory signal inducers; a process which make amnion and its epithelial and mesenchymal stem cells more suitable for tissue engineering and regenerative medicine.

show abstract

Section: Discussionsupporting

confidence: 94%

Induction of antimicrobial peptides secretion by IL-1β enhances human amniotic membrane for regenerative medicine

Tehrani

Modaresifar

Azizian

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The cryopreservation of living cells and tissues in the world of biotechnology has been developed tremendously because this process allows the recovery of large populations of eukaryotic and prokaryotic cells at very low temperatures [1, 2]. Human semen cryopreservation is one of those beneficial approaches that allows the storage of sperm and thus conserves their sperm quality [3].…”

Section: Introductionmentioning

confidence: 99%

Electromagnetic field in human sperm cryopreservation improves fertilizing potential of thawed sperm through physicochemical modification of water molecules in freezing medium

et al. 2019

View full text Add to dashboard Cite

Physicochemical properties of water molecules as the main compositions of the freezing media can be affected by the electromagnetic fled. The purpose of this study was to apply extremely low repetition rate electromagnetic fields (ELEFs) to change the molecular network of water molecules existing in freezing media used for human sperm cryopreservation. First, different time periods and pulsed electromagnetic fields were used to evaluate the physiochemical properties of water. The lowest rate of cluster size, surface tension, viscosity, and density was observed for water samples exposed to 1000 Hz ELEF for 60 min (P < 0.05) that could be results in small ice crystal formation. Therefore, this treatment was selected for further evaluations in human sperm freezing because there was minimal probability of amorphous ice crystallization in this group. To assess fertilizing potential, human semen samples were subjected to ELEF (1000 Hz) water-made freezing medium and cryopreserved. The highest percentage of total motility, progressive motility, viability, membrane integrity, mitochondrial membrane potential, DNA integrity, and TAC were obtained in frozen ELEF as compared to other groups. The percentage of viable spermatozoa (Annexin V - /PI - ) in frozen ELEF was significantly higher than in frozen control. The level of ROS was significantly lower in frozen ELEF when compared to frozen control. It can be concluded that the modification of physicochemical properties of water existing in cryopreservation media by ELEF is a suitable strategy to improve the outcome of cryopreservation.

show abstract

“…On the other hand, the cryopreservation procedure significantly lowered the levels of the antiangiogenic factors. These promising results show that the capability to induce death of cancer calls and antiangiogentic properties of the amniotic membrane are maintained even after cryopreservation [37]. Another promising feature of the amniotic membrane is its ability to reduce the formation of scar tissue -as a fetal tissue it significantly minimizes cicatrization [38].…”

Section: New Possibilities Of Application Of Grafts Prepared From Plamentioning

confidence: 79%

Preparation of Amniotic Membrane and It’s Application in the Treatment of Skin Loss and Lyell's Syndrome (Toxic Epidermal Necrolysis): Current State and New Opportunities

Klama-Baryła¹,

Łabuś²,

Kitala³

et al. 2017

J Clin Exp Dermatol Res

View full text Add to dashboard Cite

Amniotic membrane is a good protection against bacterial infections, reduces losses of proteins, fluids and electrolytes, decreases pain from burns and accelerates healing. Due to its richness in nutrients and low immunogenicity, it is often used as a skin substitute. Human amnion may be prepared in tissue banks, as biostatic or biovital grafts. There are various methods of amniotic graft preparations developed to make them efficient in surgical treatment and prevent the risk of transmission of infectious diseases. This paper presents the current clinical application of human amnion in the treatment of skin losses, such as burns to the face and Lyell's syndrome (toxic epidermal necrolysis). It also describes new applications of fetal membranes for other diseases. Placental grafts are often an alternative therapy in the situations when standard treatment does not yield desirable effects. Such grafts are especially beneficial in comparison to many other bioactive therapies, including low cost, easy manipulation, capability of promoting cell migration and proliferation and stimulation of stem cell activity.

show abstract

The effect of cryopreservation on anti-cancer activity of human amniotic membrane

Cited by 29 publications

References 27 publications

Induction of antimicrobial peptides secretion by IL-1β enhances human amniotic membrane for regenerative medicine

Induction of antimicrobial peptides secretion by IL-1β enhances human amniotic membrane for regenerative medicine

Electromagnetic field in human sperm cryopreservation improves fertilizing potential of thawed sperm through physicochemical modification of water molecules in freezing medium

Preparation of Amniotic Membrane and It’s Application in the Treatment of Skin Loss and Lyell's Syndrome (Toxic Epidermal Necrolysis): Current State and New Opportunities

Contact Info

Product

Resources

About