The effect of CPTA analogs and other nitrogenous compounds on the biosynthesis of carotenoids in Phycomyces blakesleeanus mutants

In vivo channeling of substrates in an enzyme aggregate for beta-carotene biosynthesis.

“…Wild-type Phycomyces turns red when grown in the presence of cyclase inhibitors: imidazole (6, 7), a-picoline (2-methylpyridine) (6), nicotine (8,9), and 2-(4-chlorophenylthio)triethylamine hydrochloride (CPTA) (10)(11)(12). The Carotene Analysis.…”

Carotenoid induction in orange endocarp

“…A large number of bioregulators of carotenoid biosynthesis have been developed (Hsu et al, 1975;Poling et al, 1976) and shown to have a profound effect on carotenogenesis in a number of organisms Fruit and Vegetable Chemistry Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Pasadena, California 91106. (Coggins et al, 1970;; ; Gertman and Fuchs, 1973;Elahi et al, 1975;Jahn and Young, 1975; Hayman and Yokoyama, 1976). These compounds cause accumulation of the acyclic carotene, lycopene, and induce increased carotenogenesis.…”

Chemical alteration of carotene biosynthesis in Phycomyces blakesleeanus and mutants.

“…The effects of CPTA (2-[4-chlorphenylthio]-triethylamine hydrochloride) and Cycocel on carotenogenesis in a high (3-carotene mutant strain (C115), a high lycopene strain (09), and an albino mutant (05) have been investigated (6). Similarly, the action of CPTA analogues, nitrogenous heterocyclic compounds, and various other nitrogenous compounds has also been extensively studied on the C115 strain and to a limited extent on the C9 mutant (4,5). From the C9 mutant we have been able to prepare a partially purified cell-free system capable of converting [trans-14C]geranylgeranyl pyrophosphate to phytoene, phytofluene, 4-carotene, neurosporene, and lycopene (10).…”