Chemical alteration of carotene biosynthesis in Phycomyces blakesleeanus and mutants.

Lee, T C; Rodriguez, D B; Karasawa, I; Lee, T H; Simpson, K L; Chichester, C O

doi:10.1128/aem.30.6.988-993.1975

Cited by 12 publications

(1 citation statement)

References 19 publications

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“…In our experiments, selective medium containing diphenylamine was used for selecting mutants. Diphenylamine is known to affect carotenoid synthesis in a variety of organisms (Lee and others 1975). The diphenylamine of suitable concentration in the medium could reduce or inhibit the levels of color β‐carotene in microorganism; on the contrary, increased levels of color β‐carotene showed that the mutation has occurred in the strain.…”

Section: Resultsmentioning

confidence: 99%

Optimization of β‐Carotene Production by Rhodotorula glutinis Using High Hydrostatic Pressure and Response Surface Methodology

Wang¹,

Sun²,

Han³

et al. 2007

Journal of Food Science

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Rhodotorula glutinis RG6 was treated by high hydrostatic pressure (HHP) of 300 MPa for 15 min for improving its ability of beta-carotene production. After the treatments of 5 repeated cycles, the mutant strain RG6p was obtained, beta-carotene production of which reached 10.01 mg/L, increased by 57.89% compared with 6.34 mg/L from parent strain RG6. To optimize the medium for beta-carotene fermentation by mutant RG6p, a response surface methodology (RSM) approach was used in conjunction with a factorial design and a central composite design, and the maximum yield of beta-carotene (13.43 mg/L), an increase of 34.17% compared to the control, was obtained at a pH 6.7 with an optimum medium (40 mL/250 mL) of yeast extract (4.23 g/L), glucose (12.11 g/L), inoculum (30 mL/L), tomato extract (2.5 mL/L), peanut oil (0.5 mL/L), and (NH(4))(2)SO(4) (5 g/L).

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Section: Resultsmentioning

confidence: 99%

Optimization of β‐Carotene Production by Rhodotorula glutinis Using High Hydrostatic Pressure and Response Surface Methodology

Wang¹,

Sun²,

Han³

et al. 2007

Journal of Food Science

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Effects of high hydrostatic pressure on the growth and β‐carotene production of Rhodotorula glutinis

Wang

Chen²,

Deng³

et al. 2008

Yeast

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The effects of high hydrostatic pressure (HHP) on the biomass and β-carotene biosynthesis of Rhodotorula glutinis R68 were studied. After treatment with five repeated cycles at 300 MPa for 15 min, the barotolerant mutant PR68 was obtained. After 72 h of culture, the biomass of mutant PR68 was 21.6 g/l, decreased by 8.5% compared to the parental strain R68, but its β-carotene production reached 19.4 mg/l, increased by 52.8% compared to the parental strain R68. The result of restriction fragment length polymorphism (RFLP) analysis suggested that mutant strain PR68 was likely to change in nucleic acid level, and thus enhanced β-carotene production in this strain as a result of gene mutation induced by HHP treatment.

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Vitamine und Coenzyme

Rehm

1980

Industrielle Mikrobiologie

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Chemical alteration of carotene biosynthesis in Phycomyces blakesleeanus and mutants.

Cited by 12 publications

References 19 publications

Optimization of β‐Carotene Production by Rhodotorula glutinis Using High Hydrostatic Pressure and Response Surface Methodology

Optimization of β‐Carotene Production by Rhodotorula glutinis Using High Hydrostatic Pressure and Response Surface Methodology

Effects of high hydrostatic pressure on the growth and β‐carotene production of Rhodotorula glutinis

Vitamine und Coenzyme

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