The effect of corpus allatum hormone on respiratory metabolism during larval development and metamorphosis of Galleria mellonella L.

Role of juvenile hormone in the hypermetabolic production of water revealed by the O₂ consumption and thermovision images of larvae of insects fed a diet of dry food

“…In the wax moth (Galleria mellonella), implantation of active corpora allata into the last larval instar results in a great increase in respiratory metabolism, which is associated with an increase in feeding and growth of giant supernumerary larval instars (Sehnal & Sláma, 1966). When the synthetic analogues of insect JH became available, they were found to cause a great increase in respiratory metabolism in the last larval instar of different insect species (Sláma et al, 1974).…”

“…The importance of this study was underlined by a possibility that "hot" JH-treated hypermetabolic larvae produce sufficient amounts of metabolic water for their development, which limits larval growth and survival of insects living on dry food. To this end, we reinvestigated the previous data on the O2 consumption of hypermetabolic larvae (Sehnal & Sláma, 1966;Sláma & Hodková, 1975;Sláma & Kryspin-Sørensen, 1979) and compared the results with new data obtained by thermovision monitoring of larval temperature. (Coleoptera) and Galleria mellonella (Lepidoptera) were obtained from stock cultures maintained at the Research Institute using previously described methods (Sehnal & Sláma, 1966;Sláma & Hodková, 1975).…”

“…To this end, we reinvestigated the previous data on the O2 consumption of hypermetabolic larvae (Sehnal & Sláma, 1966;Sláma & Hodková, 1975;Sláma & Kryspin-Sørensen, 1979) and compared the results with new data obtained by thermovision monitoring of larval temperature. (Coleoptera) and Galleria mellonella (Lepidoptera) were obtained from stock cultures maintained at the Research Institute using previously described methods (Sehnal & Sláma, 1966;Sláma & Hodková, 1975). Synthetic analogues of JH were applied topically in a small drop of acetone in dosages of 50 to 100 µg per larva at the beginning of the last larval instar.…”

Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase.

“…Insects. Galleria mellonella (Lepidoptera, Pyralidae) larvae were reared in constant darkness at 30 ºC on a semiartificial diet prepared as described by Sehnal and Slama (1966).…”

The effects of juvenile hormone on mitrochondrial [sic] metabolism in the Indian meal moth, Plodia interpunctella (Hubner) / by Donald Elliott Firstenburg.

“…This effect on females has been attributed to the ability of JH to stimulate developmeTil: of the female reproductive system and not due to a direct stimulation of respiratory activity (Pflugfelder, 1952;Thomsen, 1955). Sehnal and Slama (1966) implanted corpora allata into larvae of G. me Hone 11 a and monitored the oxygen consumption of the intact insects through supernumerary larval instars. They concluded that the stimulatory effect on oxygen consumption is indirect and due to the increased mass of the insects.…”