The Effect of Composition of Parenteral Solution on the Thermal Resistance of Bacillus stearothermophilus and Bacillus subtilis Spores

Penna, Thereza Christina Vessoni; Marques, Marcelo Rodrigues; Machoshvili, Irena A.; Ishii, Marina

doi:10.1007/978-1-4612-0119-9_44

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…subtilis is considered a suitable source for biosurfactant production owing to the absence of pathogenicity, which permits the use of its products in the food and pharmaceutical industries (9,10). Cell viability is easily assayed and its spores have successfully been used as biological indicators (11)(12)(13). Therefore, the development of improved strains and culturing methods of B. subtilis can provide a safe source of surfactants for waste treatment.…”

Section: Introductionmentioning

confidence: 99%

Biosurfactant production by cultivation of Bacillus atrophaeus ATCC 9372 in semidefined glucose/casein-based media

Neves

Oliveira

Kobayashi

et al. 2007

Appl Biochem Biotechnol

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Biosurfactants are proteins with detergent, emulsifier, and antimicrobial actions that have potential application in environmental applications such as the treatment of organic pollutants and oil recovery. Bacillus atrophaeus strains are nonpathogenic and are suitable source of biosurfactants, among which is surfactin. The aim of this work is to establish a culture medium composition able to stimulate biosurfactants production by B. atrophaeus ATCC 9372. Batch cultivations were carried out in a rotary shaker at 150 rpm and 35 degrees C for 24 h on glucose-and/or casein-based semidefined culture media also containing sodium chloride, dibasic sodium phosphate, and soy flour. The addition of 14.0 g/L glucose in a culture medium containing 10.0 g/L of casein resulted in 17 times higher biosurfactant production (B(max)=635.0 mg/L). Besides, the simultaneous presence of digested casein (10.0 g/L), digested soy flour (3.0 g/L), and glucose (18.0 g/L) in the medium was responsible for a diauxic effect during cell growth. Once the diauxie started, the average biosurfactants concentration was 16.8% less than that observed before this phenomenon. The capability of B. atrophaeus strain to adapt its own metabolism to use several nutrients as energy sources and to preserve high levels of biosurfactants in the medium during the stationary phase is a promising feature for its possible application in biological treatments.

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Section: Introductionmentioning

confidence: 99%

Biosurfactant production by cultivation of Bacillus atrophaeus ATCC 9372 in semidefined glucose/casein-based media

Neves

Oliveira

Kobayashi

et al. 2007

Appl Biochem Biotechnol

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“…Indicador biológico (IB) por defInição é um sistema de desafIo, contendo microrganismos viáveis, e, que possui resistência defInida a um processo de esterilização específIco (ISO 11138-1,2006, VESSONI PENNA et al, 2000.…”

Section: Indicador Biológicounclassified

Aplicação da proteína verde fluorescente (GFPuv) como indicador biológico na validação da autoclavação de soluções parenterais e da esterilização por óxido de etileno de itens termolábeis. Comparação com esporos de <i>Bacillus subtilis</i>

Ishii¹,

Penna²

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“…Por definição, um Indicador biológico (IB) é um sistema de desafio, contendo micro-organismos viáveis, e que possui resistência definida a um processo específico (ISO 11138 -1;VESSONI PENNA et al, 2000apud ISHII. M., 2006.…”

Section: Indicadores Biológicos (Ibs)unclassified

Biodegradação de benzeno por <i>Bacillus subtilis</i> em águas de rejeitos industriais e de osmose reversa; validação do sistema com grãos de kefir e Kalanchoe pinnata como indicadores biológicos (OU) Bioprocessos e bioindicadores de tratamento e monitoramento de águas: desenvolvimento e aplicações

Santos¹

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Currently, poor quality water continues to pose a major threat to human health, causing serious illnesses such as diarrhea and various types of cancer due to the ingestion of aromatic compounds and heavy metals. It is estimated that 88% of these diseases' cases are caused by unsafe water supply, sanitation and hygiene. This is largely due to poor quality control of treated water. Thus, currently, one of the major concerns in establishing water quality indicators, whether on a biological basis or through physical-chemical and mathematical methods, is to make routine ecological assessments applicable to non-specialized personnel. The present paper, aiming to offer society subsidies for the adoption of alternative technologies for treating and monitoring water quality, carried out an analysis on the potentials of kefir grains and the kalanchoe pinnata plant as bioindicators of the quality of water treated by means of Bioremediation with the microorganism Bacillus subtilis and treated water in the UPIBA. The experiments' results showed that the kefir grains generated different biological responses when submitted to different concentrations of benzene, present in bioremediated waters with B. subtilis. And trials applying K. pinnata as a bioindicator of rainwater quality and SABESP, have suggested that it may be an excellent bioindicator in eutrophic waters, that is, rich in organic matter.

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The Effect of Composition of Parenteral Solution on the Thermal Resistance of Bacillus stearothermophilus and Bacillus subtilis Spores

Cited by 3 publications

References 7 publications

Biosurfactant production by cultivation of Bacillus atrophaeus ATCC 9372 in semidefined glucose/casein-based media

Biosurfactant production by cultivation of Bacillus atrophaeus ATCC 9372 in semidefined glucose/casein-based media

Aplicação da proteína verde fluorescente (GFPuv) como indicador biológico na validação da autoclavação de soluções parenterais e da esterilização por óxido de etileno de itens termolábeis. Comparação com esporos de <i>Bacillus subtilis</i>

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