Biosurfactant production by cultivation of Bacillus atrophaeus ATCC 9372 in semidefined glucose/casein-based media

Abstract

Biosurfactants are proteins with detergent, emulsifier, and antimicrobial actions that have potential application in environmental applications such as the treatment of organic pollutants and oil recovery. Bacillus atrophaeus strains are nonpathogenic and are suitable source of biosurfactants, among which is surfactin. The aim of this work is to establish a culture medium composition able to stimulate biosurfactants production by B. atrophaeus ATCC 9372. Batch cultivations were carried out in a rotary shaker at 150 rpm and 35 degrees C for 24 h on glucose-and/or casein-based semidefined culture media also containing sodium chloride, dibasic sodium phosphate, and soy flour. The addition of 14.0 g/L glucose in a culture medium containing 10.0 g/L of casein resulted in 17 times higher biosurfactant production (B(max)=635.0 mg/L). Besides, the simultaneous presence of digested casein (10.0 g/L), digested soy flour (3.0 g/L), and glucose (18.0 g/L) in the medium was responsible for a diauxic effect during cell growth. Once the diauxie started, the average biosurfactants concentration was 16.8% less than that observed before this phenomenon. The capability of B. atrophaeus strain to adapt its own metabolism to use several nutrients as energy sources and to preserve high levels of biosurfactants in the medium during the stationary phase is a promising feature for its possible application in biological treatments.

Production of Biosurfactant Produced from Used Cooking Oil by Bacillus sp. HIP3 for Heavy Metals Removal

“…As studied by Al-Bahry et al (2013) [34], members from the genus Bacillus are considered as a group suitable for the industrial production of biosurfactants because the species within this taxon, such as Bacillus licheniformis and Bacillus subtilis , are well known as the producers of surface active metabolites. Furthermore, Neves et al (2007) [35] stated that the Bacillaceae family does not only synthesize good biosurfactants, but also has the ability to grow under anaerobic or facultative environments.…”

Biosurfactant Production by Bacillus salmalaya for Lubricating Oil Solubilization and Biodegradation

“…Many microbial ecologists have reported the potential of various Bacillus species to produce good biosurfactants under aerobic and anaerobic conditions that can be used in the food industry [8,11]. From an economic perspective, the application of low-cost substrates is an important factor for successfully developing biosurfactant production.…”

Obtaining and Evaluating Growth Parameters of Spore Bacillus Atrophaeus Atcc 9372 Using Orange Waste as a Sustainable Crop Medium

“…This difference was maintained until approximately 20 hours of cultivation (1.476 (± 0.114) g.L -1 and 0.663 (± 0.019) g.L -1 ), approaching after 3d of cultivation, with 0.713 (± 0.009) g.L -1 and 0.665 (± 0.389) g.L -1 . These profiles show that the consumption of glucose by the microorganism in bagasse is slower than in the standard medium since it must direct energy to cleave the total reducing sugars such as fructose and sucrose, adapting to the medium with the synthesis of enzymes needed to start the multiplication process (DAS NEVES et al, 2007). After 20h of cultivation, glucose consumption twice as much in TSB as in bagasse produced 60% more biomass after 4 hours, 30% more after 8 h, and reaching maximum values of 1.223 g. L -1 in bagasse and 0.917 g. L -1 in TSB (Figure 1 and 2).…”

“…at 37° C for 6 d (model 347,Fanem,São Paulo,SP,Brazil). After this period, the spore´s culture was harvested using glass beads in 0.02 mol.L -1 calcium acetate solution, adjusted to a final pH of 9.7 with 0.14% (w/v) calcium hydroxide solution, and stored under refrigeration at 4°C (DAS NEVES et al, 2007).…”