Obtaining and Evaluating Growth Parameters of Spore Bacillus Atrophaeus Atcc 9372 Using Orange Waste as a Sustainable Crop Medium

Abstract

Brazil is the world's largest producer of orange juice, generating a large amount of waste that could be used as a substrate by microorganisms, adding value to what would otherwise be discarded. Bacillus atrophaeus, they are bacteria of great interest in the pharmaceutical, hospital and industrial areas. The use of waste from orange juice processing was evaluated as an alternative culture medium to obtain orange spores. B. atrophaeus, 100 mL of water was added to the orange pomace (from 1.0 g to 20.0 g) and sterilized at 121 ºC. Tax rate 0,1 g.L -1 of spores of B. atrophaeus was inoculated in bagasse medium and incubated at 37ºC for up to 6 days. The maximum growth and productivity obtained were µ max = 0.0238 h -1 and P x = 0.0787 g. L -1 .h -1 , respectively for 5.0 g of bagasse. The values of biomass, pH, spores and thermal resistance at 102ºC were determined. An increase in pH was observed after cultivation and the spore concentration reached 1,73 x 10 9 spores. mL -1 and 5,75 x 10 9 spores.mL -1 after 3 and 6 days of incubation, respectively. The decimal reduction times determined varied from D 102C = 0,92 min at D 102C = 2,71 min. and D 102C = 1,34 min at D 102C = 3,98 min after 3 and 6 days of incubation, respectively. All media containing orange juice residues allowed the development of spores, with adequate thermal resistance, showing that these can be applied to obtain products of commercial importance.