The Effect of Combined Use of Platelet-Rich Plasma and Adipose-Derived Stem Cells on Fat Graft Survival

Seyhan, Nevra; Alhan, Doğan; Ural, Ali Uğur; Günal, Armağan; Avunduk, Mustafa Cihad; Savacı, Nedim

doi:10.1097/sap.0000000000000480

Cited by 48 publications

(24 citation statements)

References 34 publications

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“…64,65 PRP and ASCs act synergistically when combined in rodents, with better retention combined than when either are used independently. 66 The addition of PRP to human fat grafts has also been promising, with multiple reports of improved volume retention. 47,[67][68][69][70] However, findings remain far from consensus, as evidenced by a recent trial by Rigotti and colleagues, 71 which reported no benefit when adding PRP to ASC-enriched fat grafts for facial rejuvenation.…”

Section: Platelet-rich Plasmamentioning

confidence: 98%

Fat, Stem Cells, and Platelet-Rich Plasma

James

Coleman

Rubin

2016

Clinics in Plastic Surgery

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Section: Platelet-rich Plasmamentioning

confidence: 98%

Fat, Stem Cells, and Platelet-Rich Plasma

James

Coleman

Rubin

2016

Clinics in Plastic Surgery

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“…In the ‘three zone’ theory suggested by Yoshimura et al (38), the survival of fat grafts is largely dependent on adipose tissue regeneration post-transplantation, and CD34-positive cells are very likely the seed cells for adipogenic progression. Therefore, recent studies (14,39) have focused on the therapeutic effects of adipose-derived cells, including stromal vascular fraction and ADSCs, on fat grafts. These cells may improve tissue outcomes by increasing the vascularity and the secretion of growth factors that improve tissue survival.…”

Section: Discussionmentioning

confidence: 99%

Bone marrow mesenchymal stem cell-derived extracellular vesicles improve the survival of transplanted fat grafts

Huang

Feng

Zhang

et al. 2017

Molecular Medicine Reports

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Autologous fat grafting is a promising surgical technique for soft tissue augmentation, reconstruction and rejuvenation. However, it is limited by the low survival rate of the transplanted fat, due to the slow revascularization of such grafts. Previous studies have demonstrated that bone marrow mesenchymal stem cell-derived extracellular vesicles (BMSC-EVs) are proangiogenic. The present study aimed to investigate whether BMSC-EVs could improve the survival of transplanted fat grafts. Extracellular vesicles were isolated from the supernatant of cultured rat bone marrow mesenchymal stem cells, and characterized by flow cytometry and scanning electron microscopy. Their proangiogenic potential was measured in vitro using tube formation and cell migration assays. Subsequently, human fat tissue grafts, alongside various concentrations of BMSC-EVs, were subcutaneously injected into nude mice. A total of 12 weeks following transplantation, the mice were sacrificed and the grafts were harvested. The grafts from the experimental group had a higher survival rate and an increased number of vessels compared with grafts from the control group, as demonstrated by tissue volume, weight and histological analyses. Reverse transcription-quantitative polymerase chain reaction analysis indicated that the expression levels of proangiogenic factors were increased in the experimental group compared with in the control group, thus suggesting that BMSC-EVs may promote neovascularization by stimulating the secretion of proangiogenic factors. The present study is the first, to the best of our knowledge, to demonstrate that supplementation of fat grafts with BMSC-EVs improves the long-term retention and quality of transplanted fat.

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“…Recently, the combination of ASCs and PRP has been reported to yield several beneficial effects, e.g., in bone regeneration [ 21 ] and fat graft survival [ 22 ]. Particularly in wound management in pathological situations, combination therapy on ASC/PRP was suggested [ 6 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of Platelet-Rich Plasma on Viability and Proliferation in Wound Healing Processes after External Radiation

Reinders

Felthaus

Brockhoff

et al. 2017

IJMS

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Platelet-rich plasma is a current subject of studies on chronic wound healing therapy due to possible pro-angiogenic effects. Microvascular compromise represents the major component in radiogenic wound healing complications. The effects of platelet-rich plasma on irradiated cells of the cutaneous wound healing process are poorly understood so far. In this study, the interaction of endothelial cells and adipose-derived stem cells in conjunction with treatment with platelet-rich plasma is investigated in the context of radiation effects. Therefore, the expression of surface-marker CD90 and CD31 was determined. Moreover, cell proliferation and viability after external radiation was analyzed with and without treatment by platelet-rich plasma.

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The Effect of Combined Use of Platelet-Rich Plasma and Adipose-Derived Stem Cells on Fat Graft Survival

Abstract: Adipose-derived stem cells combined with PRP can enhance the survival of transplanted fat tissue.

Cited by 48 publications

References 34 publications

Fat, Stem Cells, and Platelet-Rich Plasma

Fat, Stem Cells, and Platelet-Rich Plasma

Bone marrow mesenchymal stem cell-derived extracellular vesicles improve the survival of transplanted fat grafts

Impact of Platelet-Rich Plasma on Viability and Proliferation in Wound Healing Processes after External Radiation

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