The effect of combination therapy of allicin and fenofibrate on high fat diet-induced vascular endothelium dysfunction and liver damage in rats

Li, Weihong; Wang, Daxin; Song, Guohua; Zuo, Chunxia; Qiao, Xuguang; Qin, Shucun

doi:10.1186/1476-511x-9-131

Cited by 32 publications

(31 citation statements)

References 25 publications

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“…In this study Fenofibrate did not reduce serum LDL of rats fed with atherogenic diet and this is in contrast with the study of Li et al, (2010) who found that hyperlipidemic rats treated with a high dose of Fenofibrate of 80mg/kg/day for 12 weeks reduced serum TG ,TC and LDL significantly. This indicates that the beneficial effects of Fenofibrate on serum LDL probably needs more time and higher doses would have been more informative in our study (37). Serum transaminases (ALT, AST) in this study, increased significantly in hyperlipidemic rats treated with TGS of both concentrations (0.50% and 0.75% w/w).…”

Section: Comparison Of Tgs Effects On Liver Enzymes With Rosuvastatinmentioning

confidence: 46%

“…It has been reported that Fenofibrate activates the aminotransferase gene expression, thus leading to a mild and transient elevation of aminotransferase via PPAR? through mechanisms involving increased levels of reactive oxygen species and intracellular glutathion depletion, thus leading to mitochondrial dysfunction and a perturbation of intracellular Ca++ homeostasis and also cell death (41); (37).…”

Section: Comparison Of Tgs Effects On Liver Enzymes With Rosuvastatinmentioning

confidence: 99%

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Hypolipidemic Efficacy of Trigonella Foenum Seeds in Comparison with Rosuvastatin and Fenofibrate in Hyperlipidemic Rats

Dizaye¹,

Chalaby²

2015

ME-JFM

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Background: Trigonella foenum-graecum seeds (TGS) have been historically used for the treatment of various chronic human diseases and studies concerned with application of this plant in diabetes and dyslipidemia support this hypothesis. The present study was designed to evaluate and compare the effect of different doses of TGS powder with Rosuvastatin and Fenofibrate on lipid profile, liver function enzymes, body weight and malondialdehyde (MDA) in hyperlipidemic rats.Method: Forty two rats were divided into two groups. The first group included 12 rats and received a standard diet throughout the experimental period and were subdivided into two subgroups of 6 rats each. The first subgroup served as a control group. The second subgroup received a standard diet containing TGS Powder at a concentration of 0.75% (w/w). The second group included 30 induced hyperlipidemic rats by feeding them with high cholesterol diet. They were subdivided into five subgroups each of 6 rats. First subgroup served as a positive control. The second subgroup received atherogenic diet containing TFSP at a concentration of 0.50% w/w, the third subgroup received the same diet containing TGS at a higher concentration of 0.75% w/w. The fourth and fifth subgroups received a daily dose of Rosuvastatin and Fenofibrate respectively. At the end of the treatment period (six weeks) all of these groups were subjected to various biochemical analyses of blood.Results: After six weeks of therapy, TGS of both concentrations (0.50% and 0.75% w/w) significantly reduced serum low density lipoprotein cholesterol (LDL-C), total cholesterol (TC) when compared with hyperlipidemic rats. Both concentrations of TFS (0.50%, 0.75% w/w) increased serum high density lipoprotein cholesterol (HDL-C) significantly for both normal and hyperlipidemic rats. Daily administration of Rosuvastatin of (10mg/kg) for six weeks reduced serum TC, LDL-C and triglycerides (TG) level significantly when compared with hyperlipidemic rats. Administration of Fenofibrate for six weeks markedly and significantly reduced serum TG when compared with hyperlipidemic animals. Daily use of TFS (0.75% w/w) for six weeks increased both serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) significantly for both normal and hyperlipidemic rats.Daily administration of TGS of both concentrations (0.50% and 0.75% w/w) significantly decreased serum MDA level of hyperlipidemic rats. There was a significant increase in body weight of normal rats taking diets containing TGS for six weeks.Conclusion: TGS has similar efficacy of Rosuvastatin and Fenofibrate in reducing TC. whereas, TGS was nonsignificantly more effective than Rosuvastatin and Fenofibrate in changing serum HDL-C and LDL-C.

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Section: Comparison Of Tgs Effects On Liver Enzymes With Rosuvastatinmentioning

confidence: 46%

Section: Comparison Of Tgs Effects On Liver Enzymes With Rosuvastatinmentioning

confidence: 99%

Hypolipidemic Efficacy of Trigonella Foenum Seeds in Comparison with Rosuvastatin and Fenofibrate in Hyperlipidemic Rats

Dizaye¹,

Chalaby²

2015

ME-JFM

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“…Previous studies have documented that nutritional application of various metabolic dysfunction have been conducted with the aim of regulating obesity [13-16]. There is a developing interest in the use of phytochemical compounds as medicinal alternative due to their lack of toxicity and the relative ease and cost of production.…”

Section: Introductionmentioning

confidence: 99%

Aged garlic extract enhances exercise-mediated improvement of metabolic parameters in high fat diet-induced obese rats

et al. 2012

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Aged garlic extract (AGE) is known to have a protective effect against immune system, endothelial function, oxidative stress and inflammation. We examined the effects of exercise with and without aged garlic extract administration on body weight, lipid profiles, inflammatory cytokines, and oxidative stress marker in high-fat diet (HFD)-induced obese rats. Forty-five Sprague-Dawley rats were fed either a HFD (HFD, n = 40) or a normal diet (ND, n = 5) for 6 weeks and thereafter randomized into ND (n = 5), HFD (n = 10), HFD with AGE (n = 10), HFD with Exercise (n = 10), or HFD with Exercise+AGE (n = 10) for 4 weeks. AGE groups were administered at a dose of 2.86 g/kg·body weight, orally. Exercise consisted of running 15-60 min 5 days/week with gradually increasing intensity. AGE (P < 0.01), Exercise, and Exercise+AGE (P < 0.001) attenuated body weight gain and food efficiency ratio compared to HFD. Visceral fat and liver weight gain were attenuated (P < 0.05) with all three interventions with a greater effect on visceral fat in the Exercise+AGE than AGE (P < 0.001). In reducing visceral fat (P < 0.001), epididymal fat (P < 0.01) and liver weight (P < 0.001), Exercise+AGE was effective, but exercise showed a stronger suppressive effect than AGE. Exercise+AGE showed further additive effects on reducing visceral fat and liver weight (P < 0.001). AGE significantly attenuated the increase in total cholesterol and low-density lipoprotein-cholesterol compared with HFD (P < 0.05). Exercise+AGE attenuated the increase in triglycerides compared with HFD (P < 0.05). Exercise group significantly decrease in C-reactive protein (P < 0.001). These results suggest that AGE supplementation and exercise alone have anti-obesity, cholesterol lowering, and anti-inflammatory effects, but the combined intervention is more effective in reducing weight gain and triglycerides levels than either intervention alone.

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“…Previous studies have indicated that allicin protects cells against oxidative stress by inducing the generation of antioxidant products [13]. Allicin altered the fatty acid levels of rats fed high-fat diets [14]. Recently, several studies have demonstrated that allicin decreases cholesterol and blood pressure [10, 15].…”

Section: Introductionmentioning

confidence: 99%

Allicin Decreases Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Umbilical Vein Endothelial Cells through Suppression of Mitochondrial Dysfunction and Activation of Nrf2

Zhang¹,

Pan²,

Xu³

et al. 2017

Cell Physiol Biochem

103

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Background: Allicin, a major component of garlic, is regarded as a cardioprotective agent and is associated with increased endothelial function. Methods: The effects of allicin on lipopolysaccharide (LPS)-induced vascular oxidative stress and inflammation in cultured human umbilical vein endothelial cells (HUVECs) and the mechanisms underlying these effects were studied. The protective effects were measured using cell viability, a lactate dehydrogenase (LDH) assay and cell apoptosis as indicators, and the anti-oxidative activity was determined by measuring reactive oxygen species (ROS) generation, oxidative products and endogenous antioxidant enzyme activities. HUVEC mitochondrial function was assessed by determining mitochondrial membrane potential (MMP) collapse, cytochrome c production and mitochondrial ATP release. To investigate the potential underlying mechanisms, we also measured the expression of dynamic mitochondrial proteins using western blotting. Furthermore, we evaluated the Nrf2 antioxidant signaling pathway using an enzyme-linked immunosorbent assay (ELISA). Results: Our results demonstrated that allicin enhanced HUVEC proliferation, which was suppressed by LPS exposure, and LDH release. Allicin ameliorated LPS-induced apoptosis, suppressed ROS overproduction, reduced lipid peroxidation and decreased the endogenous antioxidant enzyme activities in HUVECs. These protective effects were associated with the inhibition of mitochondrial dysfunction as indicated by decreases in the MMP collapse, cytochrome c synthesis and mitochondrial ATP release. In addition, allicin attenuated the LPS-induced inflammatory responses, including endothelial cell adhesion and TNF-α and IL-8 production. Furthermore, allicin increased the expression of LXRα in a dose-dependent manner. Allicin-induced attenuation of inflammation was inhibited by LXRα siRNA treatment. Finally, allicin activated NF-E2-related factor 2 (Nrf2), which controls the defense against oxidative stress and inflammation. Conclusions: Taken together, the present data suggest that allicin attenuated the LPS-induced vascular injury process, which may be closely related to the oxidative stress and inflammatory response in HUVECs. Allicin modulated Nrf2 activation and protected the cells against LPS-induced vascular injury. Our findings suggest that allicin attenuated the LPS-induced inflammatory response in blood vessels.

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The effect of combination therapy of allicin and fenofibrate on high fat diet-induced vascular endothelium dysfunction and liver damage in rats

Cited by 32 publications

References 25 publications

Hypolipidemic Efficacy of Trigonella Foenum Seeds in Comparison with Rosuvastatin and Fenofibrate in Hyperlipidemic Rats

Hypolipidemic Efficacy of Trigonella Foenum Seeds in Comparison with Rosuvastatin and Fenofibrate in Hyperlipidemic Rats

Aged garlic extract enhances exercise-mediated improvement of metabolic parameters in high fat diet-induced obese rats

Allicin Decreases Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Umbilical Vein Endothelial Cells through Suppression of Mitochondrial Dysfunction and Activation of Nrf2

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