The effect of collagen–chitosan porous scaffold thickness on dermal regeneration in a one-stage grafting procedure

Shi, Hua; Wang, Xingang; Wu, Shan; Mao, Zhengwei; You, Chuangang; Han, Chunmao

doi:10.1016/j.jmbbm.2013.08.031

Cited by 31 publications

(20 citation statements)

References 36 publications

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“…Due to its natural origin, this scaffold has high biocompatibility and biodegradability. Implanted CC scaffolds could be used as a substrate for initial cell attachment and physical support for tissue guidance to accelerate wound healing in vivo [3,4]. However, a CC scaffold itself lacks normal vascular networks, and angiogenesis is slow after implantation in vivo.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of Panax notoginseng Rg1 released from collagen/chitosan-gelatin microsphere scaffolds for angiogenesis

et al. 2013

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BackgroundThe emergence of skin substitutes provides a new approach for the treatment of wound repair and healing. The consistent and steady release of angiogenic factors is an important factor in the promotion of angiogenesis in skin substitutes, which usually lack, yet need, a vascular network.MethodsIn this study, ginsenoside Rg1, a natural compound isolated from Panax notoginseng (PNS), was incorporated into a collagen/chitosan-gelatin microsphere (CC-GMS) scaffold. The cumulative release kinetics were evaluated, and the effects of the released Rg1 on human umbilical vein endothelial cells (HUVECs) behavior, including proliferation, migration, tube formation, cell-cycle progression, cell apoptosis, and vascular endothelial growth factor (VEGF) secretion, were investigated. Additionally, HUVECs were cultured on the CC-GMS scaffold to test its biocompatibility. Standard Rg1 and VEGF were used as positive controls.ResultsThe results indicated that the CC-GMS scaffold had good release kinetics. The Rg1 released from the CC-GMS scaffold did not lose its activity and had a significant effect on HUVEC proliferation. Both Rg1 and VEGF promoted HUVEC migration and tube formation. Rg1 did not induce HUVEC apoptosis but instead promoted HUVEC progression into the S and G2/M phases of the cell cycle. Rg1 significantly increased VEGF secretion compared with that in the control group. HUVEC culture on the CC-GMS scaffold indicated that this scaffold has good biocompatibility and that CC-GMS scaffolds containing different concentrations of Rg1 promote HUVEC attachment in a dose- and time-dependent manner.ConclusionsRg1 may represent a new class of angiogenic agent that can be encapsulated in CC-GMS scaffolds to exert angiogenic effects in engineered tissue.

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Section: Introductionmentioning

confidence: 99%

In vitro evaluation of Panax notoginseng Rg1 released from collagen/chitosan-gelatin microsphere scaffolds for angiogenesis

et al. 2013

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“…24 Strong bonds between amino acid chains within the combination of chitosan-collagen scaffold cannot easily be degraded in the tissues, thus increasing the latters' mechanical strength and structure. 27 It can be concluded that therapy incorporating the application of a chitosan-collagen scaffold combination derived from chicken feet can increase the number of osteoblast cells and OPG expression in the healing process of bone defects in mice. …”

Section: Discussionmentioning

confidence: 99%

Scaffold combination of chitosan and collagen synthesized from chicken feet induces osteoblast and osteoprotegerin expression in bone healing process of mice

Winias¹,

Ernawati²,

Ariani³

et al. 2017

Dent. J. (Maj. Ked. Gigi)

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“…to the following equation: wound contraction rate = (1-re-epithelialization area/original wound area) Â 100% (Haifei, Xingang, & Shoucheng, 2014).…”

Section: Assessment Of Healing and Contraction Of The Skin Defectmentioning

confidence: 99%

Construction and characterization of human oral mucosa equivalent using hyper-dry amniotic membrane as a matrix

Yoshida

Koike³

et al. 2016

Archives of Oral Biology

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Background: Human amniotic membrane(HAM) as a graft material has been used in various fields. Hyperdry amniotic membrane (HD-AM) is a novel dried amniotic membrane that is easy to handle and can be preserved at room temperature without time limitation. The purpose of this study was to investigate the useful properties of HD-AM in reconstruction of the oral mucosa. Methods: Human oral keratinocytes were isolated and seeded on HD-AM in serum-free culture system. Oral mucosa equivalent (OME) was developed and transplanted onto full-thickness wound on athymic mice. The wound healing was analyzed and the OME both before and after transplantation was analyzed with hematoxylin-eosin staining and immunohistochemicial staining for Cytokines 10 (CK10), Cytokines 16 (CK16), and Ivolucrin (IVL). Results: Oral keratinocytes spread and proliferated well on HD-AM. Two weeks after air-lifting, OME had formed with good differentiation and morphology. We confirmed immunohistochemically that the expression of CK10 was positive in all suprabasal layers, as was CK16 in the upper layers, while IVL was present in all cell layers. Three weeks after transplantation to athymic mice, the newly generated tissue had survived well with the smallest contraction. The epithelial cells of newly generated tissue expressed CK10 throughout in all suprabasal layers, IVL was mainly in the granular layer, and CK16 positive cells were observed in all spinous layer and granular layer but were not expressed in the mouse skin, all of which were similar to native gingival mucosa. Conclusions: The OME with HD-AM as a matrix revealed a good morphology and stable wound healing. This study demonstrates that HD-AM is a useful and feasible biomaterial for oral mucosa reconstruction.

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The effect of collagen–chitosan porous scaffold thickness on dermal regeneration in a one-stage grafting procedure

Cited by 31 publications

References 36 publications

In vitro evaluation of Panax notoginseng Rg1 released from collagen/chitosan-gelatin microsphere scaffolds for angiogenesis

In vitro evaluation of Panax notoginseng Rg1 released from collagen/chitosan-gelatin microsphere scaffolds for angiogenesis

Scaffold combination of chitosan and collagen synthesized from chicken feet induces osteoblast and osteoprotegerin expression in bone healing process of mice

Construction and characterization of human oral mucosa equivalent using hyper-dry amniotic membrane as a matrix

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