The effect of cold atmospheric plasma on diabetes-induced enzyme glycation, oxidative stress, and inflammation; in vitro and in vivo

Rezaeinezhad, Alireza; Eslami, Pegah; Mirmiranpour, Hossein; Ghomi, Hamid

doi:10.1038/s41598-019-56459-y

Cited by 31 publications

(33 citation statements)

References 54 publications

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“…This may be because minerals are at the active site of some antioxidant enzymes. As shown in Figure 4 obtained in this study were in agreement with the results of our previous study [23]. However, in the present study, no significant reduction was observed in these two parameters.…”

Section: Discussionsupporting

confidence: 93%

“…According to the results of our study, shown in Figure 3, the activity of antioxidant enzymes in cold plasma-treated diabetic samples increased compared to untreated diabetic samples. These results were in agreement with the results of our previous work; however, in the previous study, only the activity of GPx was examined [23]. In that study, an increase in the activity of GPx was reported in the blood samples of diabetic mice exposed to cold plasma.…”

Section: Discussionsupporting

confidence: 92%

“…It has been shown that plasma is effective in improving the activity of antioxidant enzymes such as CAT and SOD [22]. In our previous study, glycated GPx, due to hyperglycemia, was exposed to cold plasma, and an increase in the activity of this enzyme was observed [23]. In this research, the effect of cold plasma, as an adjunct method, on the levels of glucose, hydrogen peroxide (H2O2), antioxidant enzymes, and some minerals was studied.…”

Section: Introductionmentioning

confidence: 93%

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The effect of cold plasma on antioxidant enzymes, minerals, and some of the levels of the biochemical parameters in the subjects with type 2 diabetes mellitus samples

Martirosyan¹,

Ghomi

Ashoori

et al. 2021

BCHD

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Introduction: Hyperglycemia in people with diabetes mellitus and its lack of control are associated with irreversible consequences. Glycation of proteins and enzymes, especially antioxidant enzymes in uncontrolled diabetes mellitus, affects these consequences. Consumption of bioactive compounds containing antioxidants and minerals as well as the use of adjunct therapies, such as cold atmospheric plasma therapy, can be effective in preventing and controlling the consequences of diabetes mellitus.Objective: In this research, we investigated whether cold plasma treatment of diabetic samples was effective in altering the activity of oxidative enzymes, some biochemical elements, and biochemical parameters.Methods: Thirty individuals with type 2 diabetes mellitus and 30 healthy individuals, as controls, participated in the study. The samples were exposed to cold argon plasma jet for 10 minutes (by a 10 kHz pulsed DC power supply with an amplitude up to 20.0 kV). The following contents of the serum samples of all participants were evaluated according to the instructions of the used kits before and after the cold argon plasma jet treatment: the activity of catalase, superoxide dismutase, and glutathione peroxidase enzymes; the concentration of glucose, hydrogen peroxide, and selenium binding protein 1 (as an indicator of blood selenium); and the concentration of copper, zinc, iron, and magnesium.Results: The activity of antioxidant enzymes and minerals significantly increased in diabetic samples treated with cold plasma (P value < 0.05). No significant changes were observed in the concentrations of glucose, hydrogen peroxide, or selenium binding protein 1 in diabetic samples treated with cold plasma.Conclusions: Using cold argon plasma jet as an adjunct method, which will reduce the glycation of enzymes and improve some minerals, can reduce the risk of diabetes complications in patients with diabetes mellitus.Keywords:Antioxidant enzymes, Cold plasma, Diabetes mellitus, Minerals.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 93%

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The effect of cold plasma on antioxidant enzymes, minerals, and some of the levels of the biochemical parameters in the subjects with type 2 diabetes mellitus samples

Martirosyan¹,

Ghomi

Ashoori

et al. 2021

BCHD

Self Cite

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“…CAP could balance the quality of the inflammatory reaction by decreasing cytokine production and increasing the formation of anti-inflammatory mediators such as IL-10, TGFβ, IL-8 13 . It is assumed that CAP modulates and restores the enzymatically (or non-enzymatically) eliminated nitrogen species (RNS/RONS) or radicals, essential for wound healing 16,26 . CAP also induces cell proliferation and neovascularization 14,15 .…”

Section: Discussionmentioning

confidence: 99%

Cold atmospheric plasma as an effective method to treat diabetic foot ulcers: A randomized clinical trial

Mirpour

Fathollah

Mansouri

et al. 2020

Sci Rep

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Cold atmospheric plasma (CAP) was shown to decrease bacterial load in chronic wounds. It was also presented as a novel approach to healing wounds in both in vitro and in vivo experiments. We aimed to examine the first randomized clinical trial for the use of CAP in diabetic foot ulcers. Patients (n = 44) were randomly double-blinded, and assigned to receive standard care (SC, n = 22) without or with CAP, to be applied three times a week for three consecutive weeks (SC + CAP, n = 22), using block randomization with mixing block sizes of four. The trial was conducted at the Diabetes Research Center in Tehran, Iran. CAP was generated from ionized helium gas in ambient air, and driven by a high voltage (10 kV) and high frequency (6 kHz) power supply. Primary outcomes were wound size, number of cases reaching wound size of <0.5, and a bacterial load after over three weeks of treatment. CAP treatment effectively reduced the fraction of wound size (p = 0.02). After three weeks, the wounds to reach fraction wound size of ≤0.5 was significantly greater in the SC + CAP group (77.3%) compared to the SC group (36.4%) (p = 0.006). The mean fraction of bacterial load counted in each session 'after CAP exposure' was significantly less than 'before exposure' measures. CAP can be an efficient method to accelerate wound healing in diabetic foot ulcers, with immediate antiseptic effects that do not seem to last long. Diabetes mellitus (diabetes) accounts for 3.9% of annual non-communicable disease-related deaths that occurred worldwide and also caused significant morbidity, impairing the patients' quality-of-life 1. Diabetic foot syndrome (DFS) is directly associated with diabetes, contributing to significant morbidity, as well as economic and social burden 2. It is estimated that approximately 25% of diabetic patients will develop DFS in their lives 3. Some authors reported that patients have a 3 to 11% annual risk of developing lower-extremity ulcers 2. Despite optimal treatment, diabetic ulcers are refractory to wound healing, with more than 50% recurring in the wounds after three years 4. Diabetic foot infection can also lead to complications, e.g., delayed healing process, systemic infections, and amputation. In addition, over 15% of patients with DFS experiences a lower limb amputation 5. The survival rate is found to be significantly lower in patients who require a lower limb amputation. The cost for two years of care with a newly-diagnosed foot ulcer is over $2,700 5. This has prompted more research and studies to identify viable alternatives or additive treatment options. Cold atmospheric plasma (CAP) is an innovative approach in wound healing. In vitro 6 and initial clinical studies for chronic wounds in animals 7,8 and humans 9-12 have shown that CAP decreases their bacterial load and promotes healing without any significant side effects on normal tissue. Additionally, CAP was found to facilitate the transformation of the chronic wound from becoming a stagnating wound or an acute healing wound through modulation of the i...

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“…Plasma medicine as a field of applied redox biology is gaining increasing interest providing a multitude of dermatological applications [ 16 ]. Novel studies present promotion of wound healing by CAP via the induction of reepithelialization, neovascularization, and inflammatory response [ 17 , 18 ], which in turn might introduce redox systems as novel treatment targets or markers during previously unrecognized local PV treatment regimes. We therefore asked whether CAP with unknown cause-and-effect mechanisms is able to restore or induce skin-located enzymatic systems such as the Trx, Grx, or Prx system ex vivo .…”

Section: Introductionmentioning

confidence: 99%

Redox Enzymes of the Thioredoxin Family as Potential and Novel Markers in Pemphigus

Sliwiak

Folwarczny

Didona

et al. 2021

Oxidative Medicine and Cellular Longevity

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Pemphigus vulgaris (PV) is a severe autoimmune blistering disease affecting both skin and mucous membranes. Its pathogenesis is related to IgG autoantibodies primarily targeting the cellular adhesion protein desmoglein (Dsg) 3, one of the major desmosome components. Impaired redox regulation is considered a major player in the pathogenesis of autoimmune diseases such as pemphigus by enhancing inflammation and breakdown of immunological tolerance by structural protein modifications. Despite many recent advances, local and systemic redox profiles that characterize the immune response in pemphigus are virtually unknown but potentially crucial in further advancing our understanding of redox-dependent modifications that eventually lead to clinical manifestation. Here, we have analyzed the individual expression pattern of four major redox enzymes that are members of the thioredoxin (Trx) fold superfamily (peroxiredoxins (Prxs) 1 and 4, glutaredoxin (Grx) 2, and Trx1) in serum and PBMCs as well as their distribution in the skin of pemphigus patients compared to healthy controls. We show that in groups of five pemphigus patients, Prx1 is upregulated in both serum and PBMCs, while its epithelial distribution remains within the spinous epithelial layer. Expression of Grx2 and Prx4 is both reduced in serum and PBMCs, while their distinct and similar expression in the skin changes from an even distribution throughout the basal layer (healthy) to ubiquitous nuclear localization in pemphigus patients. In PV patients, Trx1 is secreted into serum, and cellular distribution appears membrane-bound and cytosolic compared to healthy controls. We furthermore showed that a 3D ex vivo human skin model can indeed be used to reproduce similar changes in the protein levels and distribution of redox enzymes by application of cold atmospheric plasma. Deciphering the relationship between redox enzyme expression and autoimmunity in the context of pemphigus could be critical in elucidating key pathogenic mechanisms and developing novel interventions for clinical management.

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The effect of cold atmospheric plasma on diabetes-induced enzyme glycation, oxidative stress, and inflammation; in vitro and in vivo

Cited by 31 publications

References 54 publications

The effect of cold plasma on antioxidant enzymes, minerals, and some of the levels of the biochemical parameters in the subjects with type 2 diabetes mellitus samples

The effect of cold plasma on antioxidant enzymes, minerals, and some of the levels of the biochemical parameters in the subjects with type 2 diabetes mellitus samples

Cold atmospheric plasma as an effective method to treat diabetic foot ulcers: A randomized clinical trial

Redox Enzymes of the Thioredoxin Family as Potential and Novel Markers in Pemphigus

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