The effect of cold and heat treatments on the anther culture response of diverse rye genotypes

Tenhola-Roininen, Teija; Tanhuanpää, Pirjo; Immonen, Sirkka

doi:10.1007/s10681-005-8229-6

Cited by 23 publications

(15 citation statements)

References 33 publications

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“…Voima and the Russian short mutant EM-1 with the dominant dwarfing gene Ddw1 (Kobyliansky 1972) using anther culture (Tenhola-Roininen et al 2006). The plants were grown as previously described (Tenhola-Roininen et al 2005). Two populations (109/49 individuals) segregating for short straw were created ( Fig.…”

Section: Plant Materials and Height Measurementmentioning

confidence: 99%

Tagging the dwarfing gene Ddw1 in a rye population derived from doubled haploid parents

Tenhola-Roininen

Tanhuanpää

2009

Euphytica

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To prevent lodging and sprouting damage in rye, a dominant dwarfing gene, Ddw1, has been incorporated into breeding lines to reduce plant height in Finland. However, the inability to identify heterozygous plants makes it difficult to purify breeding lines. Doubled haploidy (EM-1 9 Voima) and bulked segregant analysis were used to search for an efficient PCR-based tool to identify homozygous short plants. In addition, SNP (single nucleotide polymorphism) markers were created from the endosperm-specific b-amylase gene and the microsatellite locus REMS1218 expressed in rye and known to be located near Ddw1. The best marker was a combination of the microsatellite REMS1218 and the SNP created from it, located 13 cM from the QTL corresponding to Ddw1. However, for a rye breeder, the SNP alone is an adequate tool to identify plants homozygous for the EM-1 allele and it can be used in selection for the desirable growth habit in rye.

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Section: Plant Materials and Height Measurementmentioning

confidence: 99%

Tagging the dwarfing gene Ddw1 in a rye population derived from doubled haploid parents

Tenhola-Roininen

Tanhuanpää

2009

Euphytica

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“…The results showed callus induction increased over control by cold pre-treatment and significantly increased (P<0.05) by 3 weeks of cold pretreatment in all tested genotypes except Yixuanhuangmi at 25°C post-incubation culture (SPSS procedure, results not shown). Tenhola-Roininen et al (2005) reported that a cold pre-treatment (4°C) was beneficial for the induction of the anther culture response in rye, as is also well documented in other cereals (Devaux et al, 1993;Głowacka and Jeżowski, 2009;Motallebi-Azar, 2010). Cold pre-treatment for 3 to 7 weeks was most effective in anther culture of barley (Huang and Sunderland, 1982;Hou et al, 1993).…”

Section: Effect Of Duration Of Cold Pre-treatment On Callus Inductionmentioning

confidence: 67%

Effects of genotypes and medium on callus induction of proso millet derived from anther culture

Wu¹,

Tianyu²,

Guojun³

et al. 2012

Afr. J. Agric. Res.

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Proso millet is a promising species for rich nutrition and drought tolerance. Factors influencing antherderived callus induction efficiency were investigated when microspores of anthers taken at the lateuninucleate to early-binucleate stage were used as explants. The results indicate that callus induction is strongly affected by genotype. Several highly responsive cultivars of proso millet with desired traits have been identified that could be directly used in crop improvement by breeding. Cold pre-treatment of spikes for three weeks, followed by 32.5°C heat treatment for a short time significantly improved callus yield. Moreover, W14 basal medium and a combination of 60 g/L sucrose with 60 g/L maltose as carbon source were optimal for anther culture of proso millet in this study. To our knowledge, this is the first report on successful regeneration of plants from pollen-derived callus in proso millet for use as a source to accelerate breeding programs.

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“…Thermal stress application (at 4EC) is commonly used to induce androgenesis in anther cultures (Immonen and Anttila, 1999;Tenhola-Roininen et al, 2005) and isolated microspore cultures of rye (Guo and Pulli, 2000;Ma et al, 2004). Thus, for the analyzed 30 genotypes of Secale cereale, low temperature of 4EC was selected as the stress factor, with spikes exposed to its action for a period from 0 to 6 weeks.…”

Section: Resultsmentioning

confidence: 99%

The effect of cold temperature stress on the viability of rye ( Secale cereale L.) microspores

Mikołajczyk

Broda²,

Weigt³

2012

bta

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Rye has been generally considered to be recalcitrant to the androgenesis induction in vitro. The haploids of Secale cereale are not easily obtainable and the isolated microspore technique application is difficult, thus is performed occasionally, and in a limited number of strains and cultivars. The aim of the conducted experiment was to assess the frequency of mechanical damage in microspores after their isolation in the mortar, and the effect of the pretreatment of spikes at 4EC on microspore viability and the course of the culture. The analyses in the isolated microspore culture were conducted on 30 genotypes of rye from two breeding programs and differing in their origin. Spikes with microspores in the unicellular stage were cut and exposed to a temperature of 4EC for a period of 2 to 42 days. Microspore observations were conducted immediately after the microspore isolation in order to assess the viability and percentage of the damaged ones. In order to study the mitotic divisions first, cultures of rye microspores were examined after 3 days. The highest microspore viability after isolation was observed for strain 1283C -91.9% and inbred line S1152/10 -91.8%, while it was the lowest for F 1 hybrid S02779/10 -79.8% and strain 1252E -84%, respectively. It was found that to maintain high (microspore -to mozna opuscic) viability, microspores must be isolated after low temperature stress (+4EC) applied for up to 7 days of culture. A considerable extension of the duration of low temperature (36-42 days) resulted in a reduction of the microspore isolation efficiency in rye (a lower number of microspores in the obtained suspension) and a slight reduction of microspore viability.

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The effect of cold and heat treatments on the anther culture response of diverse rye genotypes

Cited by 23 publications

References 33 publications

Tagging the dwarfing gene Ddw1 in a rye population derived from doubled haploid parents

Tagging the dwarfing gene Ddw1 in a rye population derived from doubled haploid parents

Effects of genotypes and medium on callus induction of proso millet derived from anther culture

The effect of cold temperature stress on the viability of rye ( Secale cereale L.) microspores

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