The effect of colchicine-specific active immunization of colchicine toxicity and disposition in the rabbit

Jm, Scherrmann; Urtizberea, M.; Pierson, Pascale David; Terrien, N.

doi:10.1016/0300-483x(89)90134-0

Cited by 29 publications

(10 citation statements)

References 19 publications

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“…2 However, the diagnostic and pharmaceutical potential of antibodies is limited because these high molecular weight proteins [immunoglobulin G (IgG), 150 kDa] do not cross either capillary or cellular barriers. 3,4 Even trunctated forms of antibodies, such as antigen binding fragments (Fab, 50 kDa) or even sFv fragments (27 kDa), are too large to easily penetrate physiological membranes.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and Organ Distribution of Cationized Colchicine-Specific IgG and Fab Fragments in Rat

Hong

Bazin-Redureau

Scherrmann

1999

Journal of Pharmaceutical Sciences

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Pharmacokinetics of cationized goat colchicine-specific polyclonal immunoglobulin G (IgG) and antigen binding fragment (Fab) (cIgG and cFab, respectively) were studied in male adult Sprague-Dawley rats and compared with those of the native proteins (nIgG and nFab). All proteins were radioiodinated by the Iodogen method, and kinetics were investigated following trichloroacetic acid (TCA) precipitation or immunoprecipitation. Deiodination and catabolism were more pronounced with the cationized than the native proteins, especially for cFab. Both cIgG and cFab in plasma decreased more rapidly than nIgG and nFab. The elimination half-lives were 52.9 and 81.8 h for cIgG and nIgG, respectively. In addition, there was a 74-fold increase in the volume of distribution and a 114-fold increase in the systemic clearance of cIgG compared with nIgG. For cFab, the volume of distribution and systemic clearance were increased 6.4- and 3.5-fold, respectively. Organ uptake of cIgG and cFab was markedly increased compared with that of nIgG and nFab, especially in kidney, liver, spleen, and lung. Renal clearance of cIgG and cFab was also increased 30- and 10-fold compared with that of nIgG and nFab, respectively. The present data suggest that cationization of colchicine-specific IgG and Fab fragments increased the organ distribution and greatly altered their pharmacokinetics. Nevertheless, the smaller molecular size of Fab versus IgG did not enhance the distribution and clearance of cFab. These data pave the way for evaluating the biological efficacy of these more tissue-organ-interactive antibodies.

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Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and Organ Distribution of Cationized Colchicine-Specific IgG and Fab Fragments in Rat

Hong

Bazin-Redureau

Scherrmann

1999

Journal of Pharmaceutical Sciences

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“…Early experimentation using a murine model demonstrated the efficacy of goat-derived colchicine specific Fab fragments (15). Earlier work by this same group using rabbit-derived antibodies showed effective sequestration of colchicine and reduced animal mortality using this therapy (16). Baud and others reported a successful outcome in a 25-year-old woman who was given 480 mg of colchicine-specific Fab fragments 40 h after ingestion of 60 mg of colchicine, but it is not available for nonexperimental use (17).…”

Section: Discussionmentioning

confidence: 95%

Colchicine-related death presenting as an unknown case of multiple organ failure

Miller

Hung

Haller

et al. 2005

The Journal of Emergency Medicine

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“…The amount of colchicine did not have any observable effect on rabbit health, despite one rabbit consuming 43.9% of the LD 50 reported in this species (Scherrmann et al, 1989). However, the LD 50 was calculated for an intravenous injection of colchicine.…”

Section: Effects Of Mes Presencementioning

confidence: 99%

“…This liquid chromatography based method showed an average concentration of 0.02% of colchicine in dried leaves. Then, the distributed dose of colchicine, depending on the proportion of MeS in the distributed hay, was compared to the median lethal dose (LD 50 ) reported at 2.75 mg/kg of live weight (Scherrmann et al, 1989).…”

Section: Measurementsmentioning

confidence: 99%

“…The economic valuation of hay from these highly valuable grasslands would be a way of ensuring their preservation at an ecological level. However, CFP grasslands can include significant proportions of Colchicum autumnale (Plantureux and Amiaud, 2010), commonly known as meadow saffron (MeS), which is highly toxic for numerous animal species (Scherrmann et al, 1989) due to the presence of the alkaloid colchicine in the plant (Poutareau and Girardin, 2002). Moreover, feed preferences of rabbits concerning rich hay is unknown and their willingness to ingest these plants or not needs to be studied.…”

Section: Introductionmentioning

confidence: 99%

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Intake of different hays with presence of Colchicum autumnale by rabbits

et al. 2017

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The ingestion of 3 hay types by growing rabbits and the consequences on animal performance were studied in a first part. Two kinds of hay from high nature value grasslands [CFP (Colchico-Festucetum pratensis) and SBR (Senecioni-Brometum racemosi)] differentiated by their botanical composition were compared to a classical agricultural hay (control). The 3 hays, offered ad libitum, were ingested at 30.6, 34.8 and 36.1 g/kg body weight (BW) respectively for SBR, CFP and the control, where the consumption of SBR hay was significantly lower. (P<0.01). The type of hay distributed did not affect the consumption of commercial feed or growth (44 g/kg BW and 36 g/d, respectively). The effect of meadow saffron concentration (Colchicum autumnale) in CFP hay was tested at 3 doses (absence, 2.75 and 15% of the distributed hay mass) with respect to their effect on ingestion and animal health. Again, no effect of the presence of meadow saffron (MeS) in the hay on these parameters was observed, even at the highest dose. However, during this second part, rabbits preferred to consume meadow saffron instead of the rest of the distributed hay (hardly any MeS was refused by rabbits at the representative dose, whereas 12% of the rest of the hay was refused, and 3.4 and 20% respectively at the maximal dose). The intake of the toxic alkaloid colchicine corresponded to 30 to 44% of the median lethal doses (LD 50 ) at the representative and maximal doses respectively. The real exposure of the rabbits was probably lower, as the absorption of ingested colchicine is reported to be around 50% of the LD 50 values which are established after an intravenous injection. Nevertheless, the absence of any effect should be interpreted with caution. The colchicine content of MeS varied greatly depending on the time of harvest and the parts of the plant taken into consideration. A late harvest (mid-June or later) would ensure (very) low concentrations of colchicine in the aerial parts of MeS. The presence of cloves on this plant should be completely avoided, due to the very high concentration of colchicine in the seeds. Moreover, long term exposure of the animals to this toxic plant may increase the risk of adverse effects. The study shows that hay distribution can aid digestion without altering the animal's performance. Moreover, the valorisation of hay from high nature value grasslands would not only add real value to the commercialisation of rabbit meat by commercial breeders, but also to pet owners on the condition that hay quality would be guaranteed.

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The effect of colchicine-specific active immunization of colchicine toxicity and disposition in the rabbit

Cited by 29 publications

References 19 publications

Pharmacokinetics and Organ Distribution of Cationized Colchicine-Specific IgG and Fab Fragments in Rat

Pharmacokinetics and Organ Distribution of Cationized Colchicine-Specific IgG and Fab Fragments in Rat

Colchicine-related death presenting as an unknown case of multiple organ failure

Intake of different hays with presence of Colchicum autumnale by rabbits

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