The effect of cleaning on blood contamination in the dental surgery following periodontal procedures

Abstract

Blood contamination of 16 surfaces in the dental surgery was investigated using the Kastle-Meyer test for haemoglobin, after three types of periodontal procedures had been performed on a total of 30 patients. The effect of cleaning surfaces contaminated by blood was investigated using the same test. Cleaning materials used in the dental surgery were tested to rule out the possibility of false positive outcomes and the sensitivity of the test was determined prior to the study. The results show a marked variation in the degree of contamination and efficacy of cleaning following treatment. Overall, root planing was associated with the most widespread and frequent blood contamination and gingival surgery the least. The surgery work surface, edge of the spittoon, aspirator tube and ultrasonic scaler handpiece into which the ultrasonic insert fits, were the most frequently contaminated surfaces. The work surface, dentist's pen, light switch and handle were cleaned most effectively. The least effectively cleaned surfaces were the water dispenser switch, aspirator tube, bracket table and ultrasonic scaler handpiece. Methods for reducing this potential source of crossinfection are discussed.

Avaliação in vitro da atividade antibacteriana da água ozonizada frente ao Staphylococcus aureus

“…A ocorrência de doenças infecto-contagiosas no ambiente profissional tem sido reconhecida há muito tempo, mas somente após a descoberta do vírus HIV e das suas formas de transmissão é que um maior enfoque foi dado para o controle de infecções na prática odontológica (CRAWFORD 6 , 1985; HAZELKORN et al 13 , 1996; EDMUNDS et al 10 , 1998). Vários microrganismos orais, como estreptococos e estafilococos, são potencialmente patogênicos e podem causar sérias infecções, especialmente em indivíduos imunocomprometidos (NEIDLE; YAGIELA 18 , 1991; MEURMAN et al 16 , 1997).…”

A study of blood contamination of Siqveland matrix bands

“…6 This method has been employed in several studies to detect blood on dental surgery surfaces. 7,8,9 The Kastle-Meyer test is highly sensitive and it has been shown that filter papers impregnated with a 1:6400 dilution or less of horse or sheep blood gives a positive reaction within 10 seconds. 9 This test is therefore applicable to the determination of effectiveness of cleaning of surgical and dental instruments after their clinical use.…”

“…7,8,9 The Kastle-Meyer test is highly sensitive and it has been shown that filter papers impregnated with a 1:6400 dilution or less of horse or sheep blood gives a positive reaction within 10 seconds. 9 This test is therefore applicable to the determination of effectiveness of cleaning of surgical and dental instruments after their clinical use. Current guidelines 10 advocate the steam autoclave as the method of choice for sterilisation of all dental instruments following cleaning.…”

An investigation of the decontamination of Siqveland matrix bands

“…Absorbance readings at a wavelength of 555 nm were taken for each sample after 70 s and a standard curve of absorbance against blood volume in the range 0-450 µl was constructed. Horse blood contains approximately the same concentration of haemoglobin as human blood, 12 hence the standard curve was directly used to estimate the blood volume of test samples.…”