The most effective method of presterilisation cleaning for hand endodontic files is a presoak in Alkazyme, an alkaline enzymatic detergent, followed by processing in a Miele G7881 washer-disinfector. This study provides up-to-date evidence that newer methods of presterilisation cleaning may fail to remove protein from endodontic hand instruments totally. This may have implications for all reusable dental instruments.
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