The effect of chitosan on stiffness and glycolytic activity of human bladder cells

Lekka, Małgorzata; Laidler, Piotr; Ignacak, Jan; Łabędź, M; Lekki, Janusz; Struszczyk, H.; Stachura, Z.; Hrynkiewicz, A.Z.

doi:10.1016/s0167-4889(01)00125-2

Cited by 81 publications

(57 citation statements)

References 38 publications

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“…6). The different cell behavior on the surface may be a result of different elasticity in cancer cells (43). The EGFR-overexpressed cells were reshaping to cover as large of an area as possible.…”

Section: Shape and Size Of Cancer Cells On Functionalized Substratesmentioning

confidence: 99%

Surface-Immobilized Aptamers for Cancer Cell Isolation and Microscopic Cytology

et al. 2010

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Exposing rare but highly malignant tumor cells that migrate from the primary tumor mass into adjacent tissue(s) or circulate in the bloodstream is critical for early detection and effective intervention(s). Here, we report on an aptamer-based strategy directed against epidermal growth factor receptor (EGFR), the most common oncogene in glioblastoma (GBM), to detect these deadly tumor cells. GBMs are characterized by diffuse infiltration into normal brain regions, and the inability to detect GBM cells renders the disease surgically incurable with a median survival of just 14.2 months. To test the sensitivity and specificity of our platform, anti-EGFR RNA aptamers were immobilized on chemically modified glass surfaces. Cells tested included primary human GBM cells expressing high levels of the wild-type EGFR, as well as genetically engineered murine glioma cells overexpressing the most common EGFR mutant (EGFRvIII lacking exons 2-7) in Ink4a/Arf-deficient astrocytes. We found that surfaces functionalized with anti-EGFR aptamers could capture both the human and murine GBM cells with high sensitivity and specificity. Our findings show how novel aptamer substrates could be used to determine whether surgical resection margins are free of tumor cells, or more widely for detecting tumor cells circulating in peripheral blood to improve early detection and/or monitoring residual disease after treatment.

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Section: Shape and Size Of Cancer Cells On Functionalized Substratesmentioning

confidence: 99%

Surface-Immobilized Aptamers for Cancer Cell Isolation and Microscopic Cytology

et al. 2010

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“…Addition of chitosan lead to an increase of stiffness of the cancer cells, whilst the non-malignant cells were not influenced by chitosan. [933].…”

Section: Filled Polyelectrolyte Capsulesmentioning

confidence: 99%

Force measurements with the atomic force microscope: Technique, interpretation and applications

Butt

Cappella

Kappl

2005

Surface Science Reports

3,198

2,949

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“…During past decades, AFM was applied to study the properties of biological samples ranging from single proteins to living cells. The stiffness of cells has been used to describe the role of cytoskeleton-associated proteins on the measured cell * corresponding author; e-mail: Malgorzata.Lekka@ifj.edu.pl elasticity [5], the effect of drugs [6] or the influence of substrate properties [7]. So far, the use of AFM has been mostly focused on the determination of Young's modulus values using the modified Hertz model, which describes in a quantitative way the elastic properties of living cells [8].…”

Section: Introductionmentioning

confidence: 99%

Energy Dissipation in the AFM Elasticity Measurements

et al. 2009

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Nowadays, it is well established that changes of cell stiffness observed by atomic force microscopy are linked with the cell cytoskeleton. Its structural and functional alterations are underlying major diseases such as cancer, inflammation or neurodegenerative disorders. So far, the use of atomic force microscopy is mostly focused on the determination of the Young modulus using the modified Hertz model. It can quantitatively describe the elastic properties of living cells, however, its value is burdened by the fact that cells are neither isotropic nor homogeneous material. Often, during the atomic force microscopy measurements, the hysteresis between the loading and unloading curves are observed which indicates the dissipation of an energy. In our studies, the index of plasticity was introduced to enumerate such effect during a single loading-unloading cycle. As the results show, such approach delivers an additional parameter describing the mechanical state of cell cytoskeleton. The analysis was performed on test samples where the mechanical properties of the melanoma cells were changed by glutaraldehyde and cytochalasin D treatments. The non-treated cells were compared with fibroblasts.

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The effect of chitosan on stiffness and glycolytic activity of human bladder cells

Cited by 81 publications

References 38 publications

Surface-Immobilized Aptamers for Cancer Cell Isolation and Microscopic Cytology

Surface-Immobilized Aptamers for Cancer Cell Isolation and Microscopic Cytology

Force measurements with the atomic force microscope: Technique, interpretation and applications

Energy Dissipation in the AFM Elasticity Measurements

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