The effect of Channa striata extract and standard eradication regimen on asymmetric dimethylarginine in Helicobacter pylori gastritis rat model

Abstract

Background and Aim: The presence of gastric mucosa or submucosa inflammation due to Helicobacter pylori leads to histological changes. Gastric injury, pro-inflammatory factors, and oxidative stress in H. pylori infection produce asymmetric dimethylarginine (ADMA), which are a competitive inhibitor of nitric oxide synthase. Investigations were carried out aimed at finding new drugs derived from natural products for the treatment of H. pylori. Channa striata is known to have in vitro anti-inflammatory and antimicrobial properties. This study aimed to investigate the effect of C. striata extract and a standard eradication regimen on ADMA levels and histological changes in the H. pylori gastritis rat model. Materials and Methods: Thirty-five male rats were randomly and equally divided into five groups. Group-1 was the negative control group and Groups-2 to 5 were H. pylori-infected groups. Groups-3 to 5 were administered C. striata extract, a standard eradication regimen, and a combination of standard eradication regimen and C. striata extract, respectively. Histological examination and serum ADMA levels were analyzed. The difference between groups was analyzed using the Kruskal–Wallis and one-way analysis of variance tests. The significance was p<0.05. Results: Serum ADMA levels and severity of gastritis were higher in infected groups compared to the negative control group (p<0.05). The severity of gastritis and mean ADMA levels in the group that received a single administration of the C. striata extract was higher than the others (p<0.05). Serum ADMA levels and severity of gastritis were significantly reduced in the group that received a combination of standard eradication regimen and C. striata extract (p<0.05). Conclusion: Single administration of C. striata extract worsens the severity of gastritis and increased serum ADMA levels in the H. pylori gastritis rat model. The administration of a combination of standard eradication regimen and C. striata extract reduces serum ADMA levels and significantly improves the severity of H. pylori gastritis rat model.

Impact of Dietary Patterns on H. pylori Infection and the Modulation of Microbiota to Counteract Its Effect. A Narrative Review

“…Snakehead fish extract supplementation was described as a useful method to eradicate H. pylori in rats, although its superiority when compared to standard first-line antibiotic treatment needs to be confirmed by further studies [ 97 ]. By contrast, the effect of the extracts from the Channa striata fish on an H. pylori -induced gastritis rat model was controversial since it worsened the severity of gastritis when administrated alone, but seemed to contribute to the improvement of the disease when combined with the standard triple therapy [ 98 ]. Another study conducted in mice reported that after six weeks of supplementation with astaxanthin from shrimp cephalothorax, H. pylori -infected mice showed an increase in splenocytes synthesis of interferon-gamma, IL-2, and IL-10, although the biological meaning of the changes in the cytokine release pattern remains to be explained [ 99 ].…”

Gastric histopathological features after the administration of omeprazole, amoxicillin, and clarithromycin in gastritis Helicobacter pylori rat model

“…Gastric injury or infection induces inflammation of the gastric mucosa. Helicobacter pylori transmission in the stomach promotes a prolonged and lasting inflammation by activating mediators adjusting the movement of neutrophils, macrophages, and other leukocytes, which in turn cause pathologic lesions of the gastric mucosa [ 13 , 27 ]. Histopathological diagnosis is a gold standard that specifies the degree of gastritis severity according to the USS.…”

Laporan Kasus: Feline Calicivirus Pada Kucing Sapi Di Klinik K and P

“…Peneliti telah mengkarakterisasi kandungan gizi yang tinggi pada ikan gabus, meliputi kandungan protein, albumin, asam amino, asam lemak, vitamin, dan mineral. Semua nutrisi tersebut sangat bermanfaat dalam pencegahan dan pengobatan beberapa penyakit termasuk yang disebabkan oleh virus (Yulizal et al, 2020).…”