The effect of Cd and Zn contents in plants on Fe binding into organic substances of spinach biomass

Pavlı́ková, Daniela; Pavlı́k, Milan; Száková, Jiřina; Vašíčková, Soňa; Tlustoš, Pavel; Balík, J.

doi:10.17221/4408-pse

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…The chlorosis may be due to Fe deficiency, because Fe binding in spinach is affected by Cd accumulation (Pavlíková et al 2002b, Martin et al 2012. Cadmium is chemically similar to certain metal elements, including Fe, Zn, Mn, Mg and Ca, and, therefore, can displace these elements from metalloproteins (Verbruggen et al 2009, Lux et al 2010.…”

mentioning

confidence: 99%

Changes in the contents of amino acids and the profile of fatty acids in response to cadmium contamination in spinach

Zemanová¹,

Pavlı́k²,

Pavlı́ková³

et al. 2015

Plant Soil Environ.

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confidence: 99%

Changes in the contents of amino acids and the profile of fatty acids in response to cadmium contamination in spinach

Zemanová¹,

Pavlı́k²,

Pavlı́ková³

et al. 2015

Plant Soil Environ.

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“…Small-plot field experiments with winter wheat (Triticum aestivum L.) cultivar Sulamit and spring barley (Hordeum vulgare L.) cultivar Jersey were run during 2007 -2010 at three sites with different soil-climatic conditions in the Czech Republic (Central Europe): Hněvčeves (50°18'46.269"N,15°42'51.552"E), Humpolec (49°32'49.604"N,15°21'6.405"E) and Ivanovice na Hané (49°18'34.209"N,17°5'18.753"E). Characteristics of the experimental sites are given in Table 1 and in Dvořák, et al (2003) and Pavlíková, et al (2002). In the trial, conventional treatment was compared to CULTAN treatment in which all the fertilizer was applied in one dose during vegetation period at the growth stage of BBCH 22 and BBCH 29-30, respectively.…”

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confidence: 99%

Influence of nitrogen injection application on zinc and iron uptake by winter wheat and spring barley

Sedlář¹,

Balík²,

Cerny³

et al. 2014

JCEA

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Influence of CULTAN method (Controlled Uptake Long Term Ammonium Nutrition) on the iron and zinc uptake by winter wheat (Triticum aestivum L.) and spring barley (Hordeum vulgare L.) was observed at four-year small-plot field experiments under conditions of the Czech Republic. No significant differences in iron and zinc concentration in grain of winter wheat as well as spring barley between conventional and CULTAN treatment were found. Neither increased supply of nitrogen fertilizer nor sulphur containing fertilizer resulted in significant differences in iron and zinc concentration in grain of winter wheat as well as spring barley at both nitrogen nutrition systems. No significant differences in sulphur concentration in aboveground biomass of winter wheat and spring barley between conventional and CULTAN treatment were recorded. Iron and zinc harvest index were not influenced by the CULTAN system. Because of no significant differences in iron and zinc concentration in grain of winter wheat as well as spring barley between conventional and CULTAN treatment, it can be assumed that nitrogen is taken up by CULTAN-treated plants in nitrate form.

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Development of a procedure for the sequential extraction of substances binding trace elements in plant biomass

et al. 2005

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This work investigates how the amounts of some important substances in a plant, and their behaviour inside the plant, depend on the levels of stress placed on the plant. To this end, model plant spinach (Spinacia oleracea L.) was cultivated on soil treated with sewage sludge. The sewage sludge contained various trace elements (As, Cd, Cu, Zn), and the uptake of these trace elements placed the plant under stress. Following this, a sequential extraction procedure was employed to determine the levels and distributions of trace elements within the most important groups of compounds present in the spinach plants. Since the usual five-step sequential extraction procedure provides only general information on the distributions of elements within individual groups of organic compounds, due to the wide range of organic compounds within the individual fractions, this scheme was extended and improved through the addition of two solvent extraction steps-a butanol step (between the ethyl acetate and methanol solvent steps) and an H(2)O step (after the methanol+H(2)O solvent step). The distributions and levels of the trace elements within the main groups of compounds in spinach biomass was investigated using this new seven step sequential extraction (water free solvents: petroleum ether (A) --> ethyl acetate (B) --> butanol (C) --> methanol (D) --> water solvents: methanol+H(2)O (1+1; v/v) (E) --> H(2)O (F) --> methanol+H(2)O+HCl (49.3+49.3+1.4; v/v/v) (G)). The isolated fractions were characterized using IR spectroscopy and the trace element contents were determined in the individual fractions. Lipophilic compounds with low contents of Cd, Cu and Zn were separated in the first two fractions (A, B). Compounds with higher As contents (11.5-12.8% of total content) were also extracted in the second fraction, B. These two fractions formed the smallest portion of the isolated fractions. Low molecular compounds from secondary metabolism and polar lipids were separated in the third (C) and fourth (D) fractions, and high molecular compounds (mainly polypeptides and proteins) separated in the fifth and sixth fractions (E, F). The addition of the H(2)O solvent step was particularly useful for separating compounds that have a significant impact on trace element bounds. The methanol fraction was dominant for all treatments, and a significant decrease in the spinach biomass separated in this fraction was observed when the soil was treated with sewage sludge. Most of the As (35.5-38.8% of total content), Cu (45.0-51.6%) and Zn (39.8-47.2%) was also determined in this fraction. The G fraction (obtained after acid hydrolysis) contained polar compounds. Most of the Cd was also found in this fraction, as was a significant amount of Zn. Non-extractable residues formed the last fraction (polysaccharides, proteins).

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The effect of Cd and Zn contents in plants on Fe binding into organic substances of spinach biomass

Cited by 4 publications

References 14 publications

Changes in the contents of amino acids and the profile of fatty acids in response to cadmium contamination in spinach

Changes in the contents of amino acids and the profile of fatty acids in response to cadmium contamination in spinach

Influence of nitrogen injection application on zinc and iron uptake by winter wheat and spring barley

Development of a procedure for the sequential extraction of substances binding trace elements in plant biomass

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