The aim of this study is to get the photon energy suitable for different thickness detector, different photon energy acts on pixel CdZnTe detector of different thickness. We can obtain the energy spectrum estimation, energy resolution and peak efficiency by the experiment and simulation with the radiation source of 241Am and 137Cs acting on pixel CdZnTe detector. From experiment results, it can be found that at the high energy of 662 keV the thicker the CdZnTe detector is, the higher the energy resolution and peak efficiency is while at the low energy of 59.5 keV tailing increases and charge is loss. It also can be found the characteristic of detector is better at the low energy when the detector thickness is thinner.