The effect of carmustine on Bergmann cells of the cerebellum

González-González, María Alejandra; Aline, Ostos-Valverde; Becerra-Hernández, A.; Sánchez-Castillo, Hugo; Martı́nez-Torres, Ataúlfo

doi:10.1016/j.neulet.2015.03.068

Cited by 2 publications

(3 citation statements)

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“…To investigate the extent of the effect of carmustine in the cerebellum, we analyzed the offspring of transgenic GFAP-eGFP male P5-P7 mice with light sheet fluorescence microscopy (Figure 1A-C). We collected data from five cerebella and determined that the most affected area was in contact with the fourth ventricle, consistent with previous findings [15]. We found that dysplasias experimentally induced by carmustine reproduced many morphological characteristics of cortical dysplasias in human pathological specimens (Figure 2D-G), including: (1) disruption of lamination, (2) heterotopias and (3) clusters of reduced morphological complexity [6,20].…”

Section: Cerebellar Cortical Dysplasiasupporting

confidence: 87%

“…The cortex of the cerebellum is divided into three cell layers: the outer molecular layer, the middle layer of Purkinje cells and the inner granular layer. Carmustine-treated mice exhibited disorganization in these cell layers, which was causally linked to changes in motor performance [ 15 ]. Furthermore, Bergmann glial cells (BG), whose soma is normally located in the Purkinje cell layer, were displaced and the complexity of their processes severely diminished.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and Calcium Signaling Alterations of Neuroglial Cells in Cerebellar Cortical Dysplasia Induced by Carmustine

Rodríguez-Arzate

Martínez-Mendoza

Rocha‐Mendoza

et al. 2021

Cells

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Cortical dysplasias are alterations in the organization of the layers of the brain cortex due to problems in neuronal migration during development. The neuronal component has been widely studied in experimental models of cortical dysplasias. In contrast, little is known about how glia are affected. In the cerebellum, Bergmann glia (BG) are essential for neuronal migration during development, and in adult they mediate the control of fine movements through glutamatergic transmission. The aim of this study was to characterize the morphology and intracellular calcium dynamics of BG and astrocytes from mouse cerebellum and their modifications in a model of cortical dysplasia induced by carmustine (BCNU). Carmustine-treated mice were affected in their motor coordination and balance. Cerebellar dysplasias and heterotopias were more frequently found in lobule X. Morphology of BG cells and astrocytes was affected, as were their spontaneous [Ca2+]i transients in slice preparation and in vitro.

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Section: Cerebellar Cortical Dysplasiasupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Morphological and Calcium Signaling Alterations of Neuroglial Cells in Cerebellar Cortical Dysplasia Induced by Carmustine

Rodríguez-Arzate

Martínez-Mendoza

Rocha‐Mendoza

et al. 2021

Cells

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“…For analyzing the EGFP-signal specifically from BG in lobule I, coronal cerebellar cryo-sections were obtained after intracardiac perfusion and cryoprotection with increasing concentrations of sucrose (10%, 20%, and 30% in PBS) (Gonza´lez-Gonza´lez et al, 2015. Five consecutive 40 mm coronal slices were collected from all 7 groups that underwent HPC at P26.…”

Section: Imagingmentioning

confidence: 99%

Response to Hypoxic Preconditioning of Glial Cells from the Roof of the Fourth Ventricle

et al. 2020

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The cerebellum harbors a specialized area on the roof of the fourth ventricle that is composed of glial cells and neurons that interface with the cerebrospinal fluid. This region includes the so-called ventromedial cord (VMC), which is composed of cells that are glial fibrillary acidic protein (GFAP)-positive and nestin-positive and distributes along the midline in association with blood vessels. We hypothesized that these cells should compare to GFAP and nestin-positive cells that are known to exist in other areas of the brain, which undergo proliferation and differentiation under hypoxic conditions. Thus, we tested whether cells of the VMC would display a similar reaction to hypoxic preconditioning (HPC). Indeed, we found that the VMC does respond to HPC by reorganizing its cellular components before it gradually returns to its basal state after about a week. This response we documented by monitoring global changes in the expression of GFAP-EGFP in transgenic mice, using light-sheet fluorescence microscopy (LSFM) revealed a dramatic loss of EGFP upon HPC, and was paralleled by retraction of Bergmann glial cell processes. This EGFP loss was supported by western blot analysis, which also showed a loss in the astrocyte-markers GFAP and ALDH1L1. On the other hand, other cell-markers appeared to be upregulated in the blots (including nestin, NeuN, and Iba1). Finally, we found that HPC does not remarkably affect the incorporation of BrdU into cells on the cerebellum, but strongly augments BrdU incorporation into periventricular cells on the floor of the fourth ventricle over the adjacent medulla. This article is part of a Special Issue entitled: Honoring Ricardo Miledi -outstanding neuroscientist of XX-XXI centuries.

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The effect of carmustine on Bergmann cells of the cerebellum

Cited by 2 publications

References 24 publications

Morphological and Calcium Signaling Alterations of Neuroglial Cells in Cerebellar Cortical Dysplasia Induced by Carmustine

Morphological and Calcium Signaling Alterations of Neuroglial Cells in Cerebellar Cortical Dysplasia Induced by Carmustine

Response to Hypoxic Preconditioning of Glial Cells from the Roof of the Fourth Ventricle

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