The effect of caffeine on cell growth and metabolism of thymidine in Escherichia coli

Sandlie, Inger; Solberg, Knut; Kleppe, Kjell

doi:10.1016/0027-5107(80)90133-5

Cited by 41 publications

(25 citation statements)

References 36 publications

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“…It has been shown to inhibit the enzymes required for DNA synthesis, for example, it inhibits the incorporation of adenine and thymidine during the synthesis of DNA 25,12,13,14 . The exact mechanism of the antimicrobial synergistic interaction observed between caffeine and amoxicillin is not yet clear.…”

Section: Discussionmentioning

confidence: 99%

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In vitro interaction between caffeine and some penicillin antibiotics against Staphylococcus aureus

Esimone¹,

Okoye²,

Nworu³

et al. 2008

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 99%

“…Some of these interactions are attributed to the ability of caffeine to form complexes with these drug molecules 11 . Others may be due to the influence caffeine on certain cellular enzymes 12,13,14 . There are reports that methylxanthines in general affect inhibitory effects of some antibacterial agents 15 .…”

Section: Introductionmentioning

confidence: 99%

In vitro interaction between caffeine and some penicillin antibiotics against Staphylococcus aureus

Esimone¹,

Okoye²,

Nworu³

et al. 2008

Trop. J. Pharm Res

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“…Caffeine in small concentrations caused stimulation of the incorporation of dNMP into DNA. At large concentrations, however, caffeine inhibited the DNA synthesis reaction, and the magnitude of this inhibition was similar to that observed by measuring thymidine incorporation in untreated cells [9]. The fact that the inhibition by caffeine was competitive with ATP strongly suggests that caffeine interferes with one or several of the energy-requiring steps in the replication process.…”

Section: Discussionmentioning

confidence: 59%

“…With regard to interaction with the genetic material DNA, it has been shown that caffeine will bind to DNA, particularly single-stranded DNA [2]. Caffeine has also been shown to affect DNA repair [3,4], to increase mutation caused by other agents [5] and to interfere with purine requiring enzymes [6-81. Work in this laboratory has shown that caffeine inhibits incorporation of thymidine into DNA in Escherichia coli and, moreover, caffeine also induces filamentous growth of the same strains [9]. Caffeine was also shown to inhibit the enzyme thymidine kinase [8].…”

Section: Introductionmentioning

confidence: 99%

Mechanism of caffeine‐induced inhibition of DNA synthesis in Escherichia coli

Sandlie¹,

Lossius²,

Sjåstad³

et al. 1983

FEBS Letters

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Caffeine inhibited DNA synthesis in toluene-treated Escherichia coli K12 strains to the same extent as in intact cells using the incorporation of [3H]thymidine as a measure of DNA synthesis. The inhibition was found to be competitive with ATP, and it was not influenced by the concentrations of deoxynucleoside triphosphates to any extent. When caffeine was added together with other DNA synthesis inhibitors such as novobiocin, nalidixic acid or actinomycin D, the inhibition in all cases was non-additive. It is suggested that caffeine inhibits one of the ATP-requiring enzymes in the DNA replication machinery, possibly DNA polymerase III or one of the DNA helicases.

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“…Dash & Gummadi (2008) also observed changes in cell morphology in E. coli and various bacterial strains in the presence of caffeine that could bring about cell disintegration. Similarly Sandlie, Solberg, & Kleppe (1980) and Esimone et al (2008) have shown that caffeine inhibits the synthesis of DNA and impairs RNA and protein synthesis. One possible explanation for this effect of caffeine could be the inhibition of bacterial cell wall synthesis by the influx of caffeine into the cells and ultimately damage to the DNA that could lead to the lysis of cells.…”

Section: Discussionmentioning

confidence: 90%

Behavior and changes in cell morphology ofEscherichia coliO157:H7 in liquid medium and skim milk in the presence of caffeine

Gyawali

Adkins

Minor

et al. 2013

CyTA - Journal of Food

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Behavior and changes in cell morphology of Escherichia coli O157:H7 in liquid medium and skim milk in the presence of caffeine Comportamiento y cambios en la morfología celular de Escherichia coli O157:H7 en un medio líquido y en leche descremada en presencia de cafeína The objective of this study was to investigate the antibacterial effect of caffeine on the behavior and changes in cell morphology of E. coli O157:H7 in a liquid medium and in skim milk. The inhibitory effect of caffeine at different concentrations was determined by inoculating E. coli O157:H7 in laboratory medium and skim milk samples. Samples were incubated at 37ºC for 48 h, and E. coli O157:H7 population was enumerated. Our results showed that caffeine significantly (P < 0.05) inhibited the growth of E. coli O157:H7 in laboratory medium and milk samples. A greater than 3.0 log CFU ml −1 inhibition was observed in milk containing 5.0 g/L caffeine within 12 h of incubation. Moreover, using flow cytometry, marked changes in the morphology of E. coli O157:H7 were also observed. Caffeine has potential as an antimicrobial agent and could be used as an effective natural additive to improve the safety of food products.Keywords: E. coli O157:H7; caffeine; inhibition; milk; flow cytometry El objetivo del presente estudio fue investigar el efecto antibacteriano que tiene la cafeína sobre el comportamiento y los cambios de la morfología celular de E. coli O157:H7 en un medio líquido y en leche descremada. Se determinó el efecto inhibitorio de distintas concentraciones de cafeína, inoculando E. coli O157:H7 en muestras de un medio de laboratorio y de leche descremada. Dichas muestras fueron incubadas a 37°C durante 48 horas, enumerándose posteriormente la población de E. coli O157:H7. Los resultados demostraron que la cafeína inhibió significativamente (P < 0,05) el crecimiento de E. coli O157:H7 tanto en las muestras del medio de laboratorio como en la muestra de leche. En la leche descremada que contenía 5,0 g/L de cafeína, se observó una inhibición mayor a 3,0 log CFU ml -1 , ocurrida dentro de las 12 horas de incubación. Asimismo, a partir del uso de una citometría de flujo se observaron cambios significativos en la morfología de E. coli O157:H7. La cafeína tiene el potencial de ser un agente antimicrobiano, por lo cual podría utilizarse como un aditivo natural efectivo para mejorar la seguridad de los productos alimenticios.Palabras Claves: E. coli O157:H7/; cafeína; inhibición; leche citometría de flujo

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The effect of caffeine on cell growth and metabolism of thymidine in Escherichia coli

Cited by 41 publications

References 36 publications

<i>In vitro</i> interaction between caffeine and some penicillin antibiotics against <i>Staphylococcus aureus</i>

<i>In vitro</i> interaction between caffeine and some penicillin antibiotics against <i>Staphylococcus aureus</i>

Mechanism of caffeine‐induced inhibition of DNA synthesis in Escherichia coli

Behavior and changes in cell morphology ofEscherichia coliO157:H7 in liquid medium and skim milk in the presence of caffeine

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