&lt;i&gt;In vitro&lt;/i&gt; interaction between caffeine and some penicillin antibiotics against &lt;i&gt;Staphylococcus aureus&lt;/i&gt;

Esimone, CO; Okoye, Festus Basden Chiedu; Nworu, CS; Agubata, Chukwuma O.

doi:10.4314/tjpr.v7i2.14675

Cited by 40 publications

(45 citation statements)

References 19 publications

(15 reference statements)

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“…In order to assess the effect of combinations of ethanolic extract and antibiotic, the MIC values for antibiotic were determined to be as a reference point for defining the interactions [26] . Synergistic effects resulting from the combination of antibiotics with various plant extracts have been studied earlier against MRSA or MSSA [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41] . From this study, ethanolic extract of E. elaterium fruits is very efficient in treating infectious diseases caused by MRSA and may also helpful for treating diseases caused by C. albicans.…”

Section: Discussionmentioning

confidence: 99%

“…Exposure to light was avoided to prevent the loss of effective ingredients. Approximately [30][31][32][33][34][35][36][37][38][39][40] g of dried material were mixed thoroughly with magnetic stirrer in 200 mL of 80% ethanol at room temperature and the mixture left for 24 h. The insoluble materials were removed by centrifugation at 10 000 rpm for 10 min at 4 曟. Then, the extract was evaporated to dryness at 37 曟.…”

Section: Plant Extractionmentioning

confidence: 99%

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Effect of ethanolic extract of Ecballium elaterium against Staphylococcus aureus and Candida albicans

Adwan

Salameh

Adwan

2011

Asian Pacific Journal of Tropical Biomedicine

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Section: Discussionmentioning

confidence: 99%

Section: Plant Extractionmentioning

confidence: 99%

Effect of ethanolic extract of Ecballium elaterium against Staphylococcus aureus and Candida albicans

Adwan

Salameh

Adwan

2011

Asian Pacific Journal of Tropical Biomedicine

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“…Dash & Gummadi (2008) also observed changes in cell morphology in E. coli and various bacterial strains in the presence of caffeine that could bring about cell disintegration. Similarly Sandlie, Solberg, & Kleppe (1980) and Esimone et al (2008) have shown that caffeine inhibits the synthesis of DNA and impairs RNA and protein synthesis. One possible explanation for this effect of caffeine could be the inhibition of bacterial cell wall synthesis by the influx of caffeine into the cells and ultimately damage to the DNA that could lead to the lysis of cells.…”

Section: Discussionmentioning

confidence: 99%

“…3, 235-241, http://dx.doi.org/10.1080/19476337.2013.834977 Silva, Nunan, & Glória, 2006Daglia, Cuzzoni, & Dacarro, 1994;Dash & Gummadi, 2008;Esimone, Okoye, Nworu, & Agubata, 2008;Ibrahim et al, 2006;Ramanaviciene, Mostovojus, Bachmotova, & Ramanavicius, 2003). Esimone et al (2008) reported the antibacterial effectiveness of amoxicillin in combination with caffeine. This suggests that caffeine could also enhance the effectiveness of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Behavior and changes in cell morphology ofEscherichia coliO157:H7 in liquid medium and skim milk in the presence of caffeine

Gyawali

Adkins

Minor

et al. 2013

CyTA - Journal of Food

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Behavior and changes in cell morphology of Escherichia coli O157:H7 in liquid medium and skim milk in the presence of caffeine Comportamiento y cambios en la morfología celular de Escherichia coli O157:H7 en un medio líquido y en leche descremada en presencia de cafeína The objective of this study was to investigate the antibacterial effect of caffeine on the behavior and changes in cell morphology of E. coli O157:H7 in a liquid medium and in skim milk. The inhibitory effect of caffeine at different concentrations was determined by inoculating E. coli O157:H7 in laboratory medium and skim milk samples. Samples were incubated at 37ºC for 48 h, and E. coli O157:H7 population was enumerated. Our results showed that caffeine significantly (P < 0.05) inhibited the growth of E. coli O157:H7 in laboratory medium and milk samples. A greater than 3.0 log CFU ml −1 inhibition was observed in milk containing 5.0 g/L caffeine within 12 h of incubation. Moreover, using flow cytometry, marked changes in the morphology of E. coli O157:H7 were also observed. Caffeine has potential as an antimicrobial agent and could be used as an effective natural additive to improve the safety of food products.Keywords: E. coli O157:H7; caffeine; inhibition; milk; flow cytometry El objetivo del presente estudio fue investigar el efecto antibacteriano que tiene la cafeína sobre el comportamiento y los cambios de la morfología celular de E. coli O157:H7 en un medio líquido y en leche descremada. Se determinó el efecto inhibitorio de distintas concentraciones de cafeína, inoculando E. coli O157:H7 en muestras de un medio de laboratorio y de leche descremada. Dichas muestras fueron incubadas a 37°C durante 48 horas, enumerándose posteriormente la población de E. coli O157:H7. Los resultados demostraron que la cafeína inhibió significativamente (P < 0,05) el crecimiento de E. coli O157:H7 tanto en las muestras del medio de laboratorio como en la muestra de leche. En la leche descremada que contenía 5,0 g/L de cafeína, se observó una inhibición mayor a 3,0 log CFU ml -1 , ocurrida dentro de las 12 horas de incubación. Asimismo, a partir del uso de una citometría de flujo se observaron cambios significativos en la morfología de E. coli O157:H7. La cafeína tiene el potencial de ser un agente antimicrobiano, por lo cual podría utilizarse como un aditivo natural efectivo para mejorar la seguridad de los productos alimenticios.Palabras Claves: E. coli O157:H7/; cafeína; inhibición; leche citometría de flujo

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“…In addition, drug synergism between known antimicrobial agents and bioactive plant extract has been reported [23]. The search for bioactive constituent of plant extracts with significant antibacterial potentials and of interaction with the currently used antibiotics may represent a promising approach toward combating the rise in bacterial resistance to these antibiotics in medical practice.…”

Section: Introductionmentioning

confidence: 99%

Potential Activity of Zinc Oxide Nanoparticles and Ethanolic Olive Leaf Extract Against Oxacillin Resistant Staphylococcus aureusin vitro

Al-Ugaili¹,

A.²,

Wohaieb³

2017

JNUS

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This study was carried out to evaluate the enhanced effect of ZnO nanoparticles and a crude ethanolic extract of olive leaves upon the antibacterial activity of oxacillin against oxacillin resistant Staphylococcus aureus (ORSA) isolate from hospital environment. A positive and induced effect for ZnO nanoparticle was detected upon the antibacterial activity of the antibiotic through the increase in the diameter of the inhibition zone for the antibiotic as it recorded (15mm) for the mixed activity to the antibiotic with nanoparticles compared with that of (10mm) for the zinc oxide nanoparticle a lone and the disappearance of the inhibition zone for the antibiotic alone. The antibacterial efficacy of the crude ethanolic olive leaf extract was evaluated alone or in combination with either oxacillin or zinc oxide nanoparticles against oxacillin resistant Staphylococcus aureus using disc diffusion method. The results revealed that olive leaf extracts (2.25-9 mg/disc) neither exhibited antibacterial activity when used alone, nor potentiated the antibacterial activity of zinc oxide nanoparticles (100µg/disc) when used in combination. However, in the presence of the highest concentration of olive leaf extract (9mg/disc), the antibacterial activity of oxacillin (1µg/disc) (as a diameter of inhibition zone) increased from zero mm to a maximum of 14 mm, suggesting a potentiation of the antimicrobial activity of oxacillin against the already existing oxacillin-resistant strain of Staphylococcus aureus.

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<i>In vitro</i> interaction between caffeine and some penicillin antibiotics against <i>Staphylococcus aureus</i>

Abstract: Purpose: The aim of this study is to evaluate the in vitro interaction of some penicillins (amoxicillin, ampicillin and benzylpenicillin)

Cited by 40 publications

References 19 publications

Effect of ethanolic extract of Ecballium elaterium against Staphylococcus aureus and Candida albicans

Effect of ethanolic extract of Ecballium elaterium against Staphylococcus aureus and Candida albicans

Behavior and changes in cell morphology ofEscherichia coliO157:H7 in liquid medium and skim milk in the presence of caffeine

Potential Activity of Zinc Oxide Nanoparticles and Ethanolic Olive Leaf Extract Against Oxacillin Resistant Staphylococcus aureusin vitro

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