The Effect of Breed, Gender, and Acid Stimulation in Dog Saliva Proteome

Lucena, Sérgio; Coelho, Ana Varela; Capela-Silva, Fernando; Tvarijonaviciute, Asta; Lamy, Elsa

doi:10.1155/2018/7456894

Cited by 12 publications

(17 citation statements)

References 33 publications

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“…Besides that, in our study, there was no difference in total protein concentration between males and females. The same result was found in the study by Lucena et al [29], in which there was no major differences between genders.…”

Section: Discussionsupporting

confidence: 89%

“…The saliva collection device has no cellulose in its composition [27] and allows immediate visual confirmation of the volume of saliva obtained; the plunger compresses the absorbent pad and the saliva is collected in the Eppendorf tube portion of the device. Foremost, differently from previous studies where the collection of saliva in dogs was performed under anesthesia with stimulation with acid [28,29], we have opted for a noninvasive collection without any other stimulation mechanism or general anesthesia. However, there were difficulties regarding the sample collection.…”

Section: Discussionmentioning

confidence: 99%

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Salivary proteomic profile of dogs with and without dental calculus

et al. 2020

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Background: Dogs' saliva is a complex mixture of inorganic and organic constituents, rich in proteins. Therefore, knowing the saliva composition of these animals is extremely important to identify the presence of proteins that may be involved in physiological and pathological mechanisms of their oral cavity. The present study aimed to characterize the proteomic profile of saliva from dogs with and without dental calculus. Results: Saliva samples were collected from 20 dogs. Before the collection, a visual clinical examination was performed and 8 subjects (40%) did not present any signs of dental calculus, while 12 (60%) presented dental calculus. After saliva collection, the samples were submitted to protein quantification (mBCA), and then they were prepared for analysis by nLC-ESI-MS/MS. A total of 658 unique proteins were identified, of which 225 were specific to dogs without dental calculus, 300 were specific to dogs with dental calculus, and 133 were common to all subjects. These proteins presented functions including transportation, immune response, structural, enzymatic regulation, signal transduction, transcription, metabolism, and some proteins perform functions as yet unknown. Several salivary proteins in dogs with dental calculus differed from those found in the group without dental calculus. Among the abundant proteins detected in periodontal affected cases, can be highlighting calcium-sensing receptor and transforming growth factor beta. Enrichment analysis reveled the presence of Rho GTPases signaling pathway. Conclusions: This research identified salivary proteins, that should be further investigated as potencial biomarkers of chronic periodontits with dental calculus formation in dogs.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Salivary proteomic profile of dogs with and without dental calculus

et al. 2020

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“…We did not find similar proteins to those from tumor tissues which was probably due to the different source of samples, as proteins in saliva could be either secretary proteins from zygomatic, parotid, mandibular and sublingual salivary glands or proteins from oral tumors. Compared with the previously reported normal canine salivary proteomics, a number of different proteins in the CP group were observed [12–15]. This was possibly owing to different groups of dogs in the study as we combined periodontitis and healthy dogs as a CP group.…”

Section: Discussionmentioning

confidence: 70%

“…In order to discover novel salivary proteins for human oral tumors, a proteomic approach has been performed [8–11]. In healthy dogs, salivary proteomic analysis as well as the comparison of canine salivary proteomics with that of healthy humans have recently been reported [12–15]. However, the study of salivary proteomics of dogs with oral diseases has not been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Salivary proteomics of canine oral tumors using MALDI-TOF mass spectrometry and LC-tandem mass spectrometry

Ploypetch¹,

Roytrakul²,

Jaresitthikunchai³

et al. 2019

PLoS ONE

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Canine oral tumors are relatively common neoplasms in dogs. For disease monitoring and early diagnosis, salivary biomarkers are appropriate because saliva collection is non-invasive and requires no professional skills. In the era of omics, matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF MS) coupled with liquid chromatography-tandem MS (LC-MS/MS) are suitable to identify potential disease-associated peptides and proteins. The present study aimed to use MALDI-TOF MS and LC-MS/MS to search for particular peptide mass fingerprints (PMFs) and conceivable biomarkers in saliva of dogs with early- and late-stage oral melanoma (EOM and LOM, respectively), oral squamous cell carcinoma (OSCC), benign oral tumors (BN), and periodontitis and healthy controls (CP). Pooled saliva samples in each group were used to be representative of population change. Unique PMFs were obtained and specific peptide fragments were sequenced by LC-MS/MS and BLAST-searched with mammalian protein databases. Seven peptide fragments appeared in the tumor groups (EOM, LOM, OSCC and BN) at 1096, 1208, 1322, 1794, 1864, 2354 and 2483 Da, two peptide fragments appeared in the LOM and OSCC groups at 2450 and 3492 Da, and in the CP controls at 2544 and 3026 Da. Also, protein–chemotherapy drug interaction networks were exhibited. Using western blot analysis, the expression of sentrin-specific protease 7 (SENP7), a peptide fragment at 1096 Da, in OSCC was significantly increased, as was the expression of TLR4, a peptide fragment at 3492 Da, in LOM and OSCC, compared with the CP group. The expression of nuclear factor kappa B (NF-κB), a TLR4 partner, was notably increased in OSCC compared with CP, BN and EOM. The expression was also enhanced in LOM compared with EOM. Expressed protein sequences from western blots were verified by LC-MS/MS. Western blots were then performed with individual samples in each group. The results showed the elevated expression of TLR4 in LOM and OSCC, compared with that in CP and BN, the increased expression of NF-κB in LOM and OSCC, compared with CP and in LOM compared with BN, and the enhanced expression of SENP7 in LOM and OSCC, compared with that in CP and BN. In conclusion, discrete clusters of EOM, LOM, OSCC, BN and CP groups and potential protein candidates associated with the diseases were demonstrated by salivary proteomics. Western blot analysis verified SENP7, TLR4 and NF-κB as potential salivary biomarkers of canine oral tumors.

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“…In the present study, 83% of the detected proteins were reported previously in canine saliva using proteomic approaches [23], while 51 identified proteins have never been reported before for dog saliva. At least in part, this can be attributed to different methodologies and dog breeds used among the diverse studies, since different salivary proteomes were described in dogs of different breeds [24,25].…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of saliva from dogs with and without obesity-related metabolic dysfuntion

Lucena

Coelho

Anjo

et al. 2019

Journal of Proteomics

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Dogs develop only some of the components of the human metabolic syndrome (MetS). Thus, in order to study possible MetS-related alterations in dogs, human MetS criteria were adapted to define canine MetS or so-called obesity-related metabolic dysfunction (ORMD). The main objective of this study was to identify changes in the salivary proteome of obese dogs with ORMD in comparison with obese dogs without ORMD which may constitute potential salivary biomarkers for assessing ORMD. In a first phase, 12 adult obese dogs with ORMD (N = 6) and without ORMD (N = 6) were included in the study. Subsequently, and with the aim of validating and strengthening the results, additional 12 obese dogs (6 with and 6 without ORMD) were tested in an independent experiment following the same protocol. Saliva samples were subjected to a quantitative proteomics analysis and the levels of nine salivary proteins were found to be significantly different between groups, among them those which had greatest fold-change were proteins involved in glycolysis and oxidative stress. In conclusion, despite metabolic syndrome to include different combinations of diseases, the observation of differences in salivary proteome suggests a potential of this fluid to understand the pathophysiology of the disease. Significance: This is the first study evaluating proteomes of saliva in dogs, as a non invasive sample, in order to increase knowledge about the metabolic/physiopathological changes related to obesity-related metabolic dysfunction (ORMD) together with the identification of potential biomarkers for its diagnosis. As approximately 20% of dogs with naturally occurring obesity were described to suffer ORMD associated with insulin resistance and hypoadiponectinemia, the fact that indicate possible links between ORMD and associated diseases.

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The Effect of Breed, Gender, and Acid Stimulation in Dog Saliva Proteome

Cited by 12 publications

References 33 publications

Salivary proteomic profile of dogs with and without dental calculus

Salivary proteomic profile of dogs with and without dental calculus

Salivary proteomics of canine oral tumors using MALDI-TOF mass spectrometry and LC-tandem mass spectrometry

Comparative proteomic analysis of saliva from dogs with and without obesity-related metabolic dysfuntion

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