The effect of bovine serum albumin on the in vitro inhibition of chemotaxis by anti-inflammatory agents

Abstract

The anti-inflammatory agents, phenylbutazone, naproxen and niflumic acid inhibited in vitro rabbit peritoneal neutrophil chemotactic responsiveness to Escherichia coli derived chemotactic factor/s when added to cells suspended in 0.1% bovine serum albumin (BSA). Inhibition of chemotaxis by these drugs was markedly diminished as the BSA concentration was increased to 2%. Experiments with phenylbutazone demonstrated that inhibition of chemotaxis could be observed using neutrophils suspended in 2% BSA by either increasing the drug concentration or by preincubating the cells suspended in 0.1% with the drug prior to the addition of the additional BSA. These data suggest that protein binding can prevent in vitro inhibition of neutrophil chemotaxis by anti-inflammatory agents.

THE EFFECTS OF SOME ANTIRHEUMATIC DRUGS ON AN in vitro MODEL OF HUMAN POLYMORPHONUCLEAR LEUCOCYTE CHEMOKINESIS

“…This discrepancy may be explained at least in part, by the relative lack of protein in the agarose micro-droplet system compared to the capillary tube method, which includes 10% horse serum. Competitive antagonism of the inhibitory actions of several anti-inflammatory drugs by increasing bovine serum albumin concentrations has been demonstrated (Rivkin, 1977). Alclofenac, a non-steroidal, acidic analgesic and antiinflammatory drug (Lambelin, Roba, Gillet & BuuHoi, 1970) also inhibited chemokinetic migration.…”

Chemotaxis of Human Polymorphonuclear Leukocytes under Agarose: Lack of Requirement for Media Protein and Differential Effects of Buffer and Agarose Type on Locomotion

“…Such observations have led to theories that chemoattractants must be bound to a carrier protein in order to activate cell receptors (12), although more recent studies suggest that proteins enhance locomotion of cells by modulating their interaction with artificial substrates ( 1 3) and therefore indirectly facilitate chemotaxis. Studies of chemoattractants, helper factors (14), and pharmacological agents (15) are complicated by possible interaction with media protein in addition to the variable and uncertain effects on PMN locomotion of proteins themselves.…”