1981
DOI: 10.3181/00379727-167-41190
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Migration and chemotaxis of human polymorphonuclear leukocytes (PMN) under agarose was studied in the presence and absence of various proteins. Contrary to previous reports, chemotaxis could be elicited in a protein-free medium using both complement-derived factors and fMet -Leu -Phe. Cell movement was critically dependent on the choice of agarose and buffer system. Addition of human serum or albumin produced marked chemokinesis while gelatin had no effect. These results indicate that media protein is a method… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
4
0

Year Published

1982
1982
2003
2003

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 7 publications
(4 citation statements)
references
References 8 publications
0
4
0
Order By: Relevance
“…By varying the substrate composition and reducing the stiffness of the overlay, it may be possible to improve the movement of mammalian cell lines. In fact, Mollison et al (9) have shown that varying the concentration of agarose affects the under agarose migration distance of PMNs.…”
Section: Effect Of Agarose Concentration On Cell Movementmentioning
confidence: 99%
“…By varying the substrate composition and reducing the stiffness of the overlay, it may be possible to improve the movement of mammalian cell lines. In fact, Mollison et al (9) have shown that varying the concentration of agarose affects the under agarose migration distance of PMNs.…”
Section: Effect Of Agarose Concentration On Cell Movementmentioning
confidence: 99%
“…Slowly diffusible chemotaxins such as LTB4 and C5a were put in the wells 1 hr before cells, while fMLP was placed in wells at the same time as cells (13). Cells were incubated for 2.5 hr at 37℃, fixed at 4℃ with 2.5% glutaraldehyde/1.0% paraformaldehyde in Millonig' s phosphate buffer, stained with Accustain (Sigma), and the leading front measured using a Nikon inverted microscope equipped with a calibrated eyepiece reticle (14). Cells in the leading front were identified as neutrophils by standard morphologic criteria.…”
Section: Methodsmentioning
confidence: 99%
“…The top well contained 10 ,u1 of the chemoattractant casein (2 mg/ml), and the bottom well contained serum-free minimal essential medium modified for Spinner culture (GIBCO). In some experiments, FMLP (prepared as described above) in a concentration (10-6 M) used by others (30,43) served as the chemoattractant. The slides were incubated at 37°C in a humid atmosphere containing 5% CO2 in air for 8 h.…”
Section: Methodsmentioning
confidence: 99%