The effect of bone marrow stromal cells on the cloning of human leukemia/lymphoma cell lines

Abstract

The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. In general, the cloning efficiency in microwells was greater than in soft agar. The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. It was concluded that cloning of leukemia and lymphoma cell lines in microwells was the preferred method and that similar techniques could improve the cloning of fresh leukemia and lymphoma cells.

Radiation Sensitivity of Human Malignant Lymphocytes

“…However, the main disadvantage of the colony formation in soft agar method is the inability of many established leukaemidymphoma cell lines, particularly pre-B leukaemia cells, to form colonies in agar (10,12). Because of these disadvantages, previous workers have developed the growth extrapolation method to study the radiation sensitivity of leukaemidymphoma cell lines (3,4).…”

“…These methods do not assess the effect of radiation on the true clonogenic malignant lymphocyte. Recently we described a high-efficiency limiting dilution technique to clone established leukaemia and lymphoma cell lines in microwells with the addition of irradiated bone marrow stromal cells as 'feeder' cells (12). The aim of the present investigation was to study the sensitivity of established human leukaemia and leukaemia lymphoma cell lines to roentgen irradiation.…”

Adhesion of human lymphoid cell lines to immobilized carbohydrates and to bone‐marrow stromal cell layers by surface sugar receptors

“…Such a cell-contact-dependent influence does not operate only for normal cells within this lineage but also for the growth of lymphoma cells from patients and cells from a human T lymphoblastic line (Seshadri et al, 1984;Gallardo et a/., 1985). It is also noteworthy that cellular interactions with the stromal cell layer have apparently played a critical role in the establishment of human B-lymphoblastoid cell lines (Juneja et a/., 1986;Gabius et a/., 1991).…”

“…Intimate cellular contact with the adherent stromal cell layer is a requirement for the proliferation of B precursor cells in long-term lymphoid-permissive culture systems (Kierney and Dorshkind, 1987;Dorshkind, 1990;Ryan et a/., 1990). Such a cell-contact-dependent influence does not operate only for normal cells within this lineage but also for the growth of lymphoma cells from patients and cells from a human T lymphoblastic line (Seshadri et al, 1984;Gallardo et a/., 1985). It is also noteworthy that cellular interactions with the stromal cell layer have apparently played a critical role in the establishment of human B-lymphoblastoid cell lines (Juneja et a/., 1986;Gabius et a/., 1991).…”

Rapid fluorometric detection of drug resistant tumour cells

“…This latter source of error might bias the estimation of the mutation rate in favour of more malignant cells, which generally have higher cloning efficiencies (Elmore et al, 1983). Morley et al (1983) and Albertini et al (1982) (Seshadri et al, 1984).…”