Adhesion of human lymphoid cell lines to immobilized carbohydrates and to bone‐marrow stromal cell layers by surface sugar receptors

Abstract

Cell-surface sugar receptors may participate in interactions of lymphoid cells that influence their adhesive properties and proliferation. Their expression on cells of the pre-B line BLIN-I, the B-lymphoblastoid line Croco II, the myeloma line RPMI 8226 and the T-lymphoblastoid line CCRF-CEM was monitored with a panel of 14 types of chemically glycosylated E. coli beta-galactosidase at a non-saturating ligand concentration. Quantitative differences were determined for the capacity of the different cell types to bind constituents of the carbohydrate part of glycoconjugates. They were corroborated by analyses of binding for lactose-, beta-N-acetylgalactosamine-, beta-N-acetylglucosamine- and fucose-exposing neoglycoenzymes up to saturation levels. Values of dissociation constants of the tetrameric enzyme were in the range of 3-300 nM. Several types of sugar receptor led to carbohydrate-inhibitable adhesion of cells to 6 types of nitrocellulose-immobilized neoglycoprotein, their effectiveness being most obvious for the myeloma cells. Analyses of the carbohydrate-ligand-mediated adhesion of the other cell types revealed a comparatively decreased response. Only a few carbohydrates among the 7 types tested were effective in reducing cell adhesion to a far more complex ligand-bearing matrix than immobilized neoglycoproteins, namely bone-marrow stromal cell layers: sialic acid and N-acetylgalactosamine for B-lymphoblastoid cells and rhamnose for pre-B cells. These cellular interactions may encompass sugar receptors on the stromal cells and other types of molecular recognition in addition to the detected activities on the lymphoid cells.

Are Differences in the Expression of Carbohydrate-Binding Sites (Endogenous Lectins) Responsible for Recurrences of Benign Tumours of the Parotid Gland?

“…It is interesting to note the possibility of therapeutic implications such as blocking receptors by the specific sugar solutions to prevent recur rences of PA, like to prevent bacterial or tumour cell adhe sion [11][12][13].…”

Sialyl LewisX carbohydrate is expressed differentially during avian lymphoid cell development

“…Several extracellular matrix macromolecules such as fibronectin or collagens participate in the adhesion of lymphocytes to the stroma. Different carbohydrate structures which support the attachment of different cell lines to the stroma have also been described [3]. Sialyl LewisX (sLeX) carbohydrate is present in many glycoproteins and is recognized by the selectin family of cell surface adhesion molecules [4].…”

Animal Lectins as Cell Adhesion Molecules

“…As discussed by Plendl and Sinowatz [1998] in this issue, the development of the intricate network of axonal projections in the olfactory bulb is dependent on galectin-1 [Puche et al, 1996]. Such molecular events explain the inhibition of cell adhesion by galectin-reactive sugars seen in assays such as rosette formation with certain types of tumor cells or adhesion to bone marrow stromal cells [Gabius et al, 1985a[Gabius et al, , 1990[Gabius et al, , 1993bRaz and Lotan, 1987]. A deliberate focus on such possible roles is presented in figure 10.…”