The Effect of Bone Marrow Mononuclear Cells on Lung Regeneration and Apoptosis in a Simple Model of Pulmonary Emphysema

Abstract

BackgroundIn severe chronic stages of emphysema the only treatment is lung transplantation. SO, an urgent need exists for the development of effective treatments. Stem cells therapy arises as a new therapeutic approach.Aim of the WorkTo investigate whether bone marrow mononuclar cells (BMMNCs) can promote lung regeneration and decrease apoptosis in lipopolysaccharide (LPS) induced pulmonary emphysema in C57Bl/6 mice.Material and Methods14 weeks old female mice (C57Bl/6), weighing around 25 g were used in this study. The mice were divided into 4 groups (10 in each group): group A: mice received no treatment, group B: mice received intranasal instillation of LPS with no further treatment, group C: mice received intranasal instillation of LPS then given a dose of BMMNCs and evaluated 21 days later and group D: the mice that received intranasal instillation of LPS then given a dose of Dulbecco’s Modified Eagle’s Medium (DMEM) and evaluated 21 days later. Imaging analysis was done using imagej program. To measure apoptotic index, Anti–caspase 3 polyclonal antibody staining was done.ResultsAnalysis of the mean of airspace equivalent diameters (D0) and its statistical distribution (D1) for the different groups allowed to observe that group treated with BMMNCs (group C) showed the significant improvement in D0 and D1 than the group received LPS only (group B). Analysis of apoptotic index showed significant difference between BMMNCs treated group (group C) and that received LPS only (group B).ConclusionsBMMNCs effectively promote lung regeneration and reduction of apoptosis in pulmonary emphysema.

Therapeutic effects of bone marrow-derived mononuclear cells from healthy or silicotic donors on recipient silicosis mice

“…In addition to the effects of TGF-β on silica-induced lung fibrosis, the increased apoptotic cell counts in lung parenchyma have been correlated to increased TGF-β levels [ 11 , 27 , 37 ]. Some studies that carried out BMMCs transplantation have demonstrated anti-apoptotic effects in different experimental models [ 38 , 39 ], and a previous study from our group has shown that repeated administration of bone marrow-derived cells reduced mRNA expression of pro-caspase 3, as well as apoptotic cell counts in lung parenchyma and granulomas [ 40 ]. Here, we have observed that both BMMC-healthy and BMMC-sil reduced apoptotic cell counts in the lung parenchyma, but not in granulomas.…”