The effect of Aspergillus oryzae fermentation extract on the anaerobic fungi Neocallimastix frontalis EB 188, Piromyces communis DC 193 and Orpinomyces ssp. RW 206:  generalized effects and component analysis

Harper, Elvin; Welch, R. P.; Lara, D. Contreras; Chang, Jen-Yuan; Calza, R. E.

doi:10.1007/s002530050768

Cited by 9 publications

(13 citation statements)

References 16 publications

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“…Cultures grown with xylose as the carbon source contained a greater number of branches and sporangia and therefore possessed a denser rhyzoidal development than glucose‐grown cultures ( Barichievich & Calza 1990b). Evidence of reduced distance between rhyzoid branches (and therefore increased overall fungal surface area) is consistent with previous findings ( Harper et al . 1996 ; Welch et al .…”

Section: Discussionsupporting

confidence: 92%

“…In the same study, it was demonstrated that stimulation occurred beyond that expected of nutritional factors and that furthermore, autoclaving or boiling extract did not affect its ability to stimulate fungi, yet this harsh treatment would probably have inactivated most enzymes. In this report, Neocallimastix frontalis EB188 is used as an in vitro tester system ( Harper et al . 1996 ; Welch et al .…”

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confidence: 99%

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Fermentation extract effects on the morphology and metabolism of the rumen fungus Neocallimastix frontalis EB188

1999

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. 1999. The effects of Aspergillus oryzae fermentation extract, Amaferm TM , on the rumen fungus Neocallimastix frontalis EB188 were studied. The secretion of cellulase was increased by 67% and rhyzoid development was increased 3·8-fold in the presence of extract. Strength of fungal response increased in a dose-dependent manner and demonstrated a positive correlation between cell surface area and enzyme secretion. Above certain concentrations of extract, however, the development of the fungus and enzyme secretions remained at control values or slightly diminished. Supernatant fluid appearance of the intracellular enzyme, malate dehydrogenase, paralleled the secretion of cellulase both in the presence and absence of extract. Ether solubilization of extract demonstrated that the active component(s) possessed a moderately polar value between 2·7 and 2·8. Thin layer chromatography separated extract into inert, inhibitory and intensely stimulating fractions. These results support the idea that by accelerating fungal growth and metabolism, Amaferm TM increases the rate (or extent) of fibre degradation caused by rumen fungi and that this, in turn, may contribute to enhanced animal performance.

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Section: Discussionsupporting

confidence: 92%

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confidence: 99%

Fermentation extract effects on the morphology and metabolism of the rumen fungus Neocallimastix frontalis EB188

1999

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“…A prebiotic, on the other hand, is a nondigestible nutritional compound that selectively stimulates the growth of the endogenous microflora within the digestive tract (Walker and Duffy, 1998). Both of these types of compounds have been shown by many researchers to selectively increase populations of beneficial microorganisms within the rumen (Newbold et al, 1995;Harper et al, 1996;Yoon and Stern, 1996;Beharka and Nagaraja, 1998;Krehbiel et al, 2003).…”

Section: Exp 1 Animal Performancementioning

confidence: 93%

Effects of diet and Aspergillus oryzae extract or Saccharomyces cervisiae on growth and carcass characteristics of lambs and steers fed to meet requirements of natural markets1,2

Zerby

Bard

Loerch

et al. 2011

Journal of Animal Science

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Two studies were conducted to determine the effects of diet and feed additive on growth and carcass characteristics of lambs and cattle destined for all natural markets. In Exp. 1, 48 Dorset × Hampshire lambs (initial BW 29.4 ± 0.1 kg) were used in a randomized complete block experiment to determine the effects of Aspergillus oryzae extract, Amaferm (AMF) supplementation (1 g/d) in an 85% concentrate diet on growth and carcass characteristics. Lambs were allotted to 12 pens (4 lambs per pen), and blocked by sex and BW. Lambs were fed until the average BW of each pen reached a target BW (55.4 kg for wethers and 50.0 kg for ewes), at which time the entire pen of lambs was slaughtered. Amaferm resulted in a greater (P=0.07) G:F. In Exp. 2, 168 crossbred steers (initial BW 300 ± 0.7 kg) were used in a trial with a 3 × 2 factorial arrangement of treatments to examine the effects of 0.5 g/d of Saccaromyces cervisiae boulardii CNCM 1079-Levucell SB (LEV), or 3 g/d of AMF with 2 corn sources, dry whole-shelled corn or high moisture corn, on growth and carcass characteristics. Neither LEV nor AMF improved (P>0.10) carcass characteristics compared with control or non-feed-supplemented steers. Addition of LEV to high-concentrate, corn-based diets did not improve (P>0.10) growth performance of feedlot steers. However, addition of AMF to a diet composed of dry whole-shelled corn resulted in an improvement (P<0.05) in G:F (0.208 vs. 0.194). Results indicate that at the amounts fed, AMF may improve G:F for lambs and steers fed dry corn-based finishing diets.

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“…The major effect was for soluble compounds with a molecular weight of less than 30 kDa to stimulate the rumen microbial fermentation and increment the growth of the mixed rumen. Harper et al. (1996) observed that the molecular mass of the component(s) extracted from Ao probably exceeded the 2 kDa cut‐off of the membrane.…”

Section: Discussionmentioning

confidence: 98%

Influence of an aerobic fungus grown on solid culture on ruminal degradability and on a mixture culture of anaerobic cellulolytic bacteria

Hernández-Díaz

Pimentel-González

Figueira

et al. 2010

Animal Physiology Nutrition

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In this work, the effect of a solid fungal culture of Aspergillus niger (An) grown on coffee pulp on the in situ ruminal degradability (RD) of corn stover was evaluated. In addition, the effect of its extracts on the in vitro dry matter disappearance (IVDMD) and on a mixed culture of anaerobic cellulolytic bacteria (MCACB) was also investigated. The solid ferment was a crude culture of An, grown on coffee pulp. Regarding in situ RD, a significant difference (p < 0.05) was found between treatment with 200 g/day of the solid culture and control (no solid culture added) on dry matter, crude protein and neutral detergent fibre on RD. All the water extracts (pH 4, 7 and 10) enhanced IVDMD and stimulated the cellulolytic activity on a MCACB. Ultrafiltration results showed that active compounds with a molecular weight lower than 30 kDa were responsible for the effect on MCACB. Such results suggest that the effects of the solid An culture in RD are related to the presence of water soluble compounds having a molecular weight lower than 30 kDa.

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The effect of Aspergillus oryzae fermentation extract on the anaerobic fungi Neocallimastix frontalis EB 188, Piromyces communis DC 193 and Orpinomyces ssp. RW 206: generalized effects and component analysis

Cited by 9 publications

References 16 publications

Fermentation extract effects on the morphology and metabolism of the rumen fungus Neocallimastix frontalis EB188

Fermentation extract effects on the morphology and metabolism of the rumen fungus Neocallimastix frontalis EB188

Effects of diet and Aspergillus oryzae extract or Saccharomyces cervisiae on growth and carcass characteristics of lambs and steers fed to meet requirements of natural markets1,2

Influence of an aerobic fungus grown on solid culture on ruminal degradability and on a mixture culture of anaerobic cellulolytic bacteria

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