Fermentation extract effects on the morphology and metabolism of the rumen fungus Neocallimastix frontalis EB188

Abstract

. 1999. The effects of Aspergillus oryzae fermentation extract, Amaferm TM , on the rumen fungus Neocallimastix frontalis EB188 were studied. The secretion of cellulase was increased by 67% and rhyzoid development was increased 3·8-fold in the presence of extract. Strength of fungal response increased in a dose-dependent manner and demonstrated a positive correlation between cell surface area and enzyme secretion. Above certain concentrations of extract, however, the development of the fungus and enzyme secretions remained at control values or slightly diminished. Supernatant fluid appearance of the intracellular enzyme, malate dehydrogenase, paralleled the secretion of cellulase both in the presence and absence of extract. Ether solubilization of extract demonstrated that the active component(s) possessed a moderately polar value between 2·7 and 2·8. Thin layer chromatography separated extract into inert, inhibitory and intensely stimulating fractions. These results support the idea that by accelerating fungal growth and metabolism, Amaferm TM increases the rate (or extent) of fibre degradation caused by rumen fungi and that this, in turn, may contribute to enhanced animal performance.

Effects of addition of Aspergillus oryzae culture and 2‐hydroxyl‐4‐(methylthio) butanoic acid on milk performance and rumen fermentation of dairy cows

“…To our knowledge, this study is the first to show an in vivo increase in ruminal F. succinogees and R. flavefaciens following AOC supplementation. The effects of AOC on stimulating rumen fermentation were consistent with the results of previous studies (Beharka & Nagaraja ; Chang et al ). The improvement of CMCase and amylase may be the result of increase of fungi, F. succinogees and R. flavefaciens .…”

Influence of an Anaerobic Fungal Culture (Orpinomyces sp.) Administration on Growth Rate, Ruminal Fermentation and Nutrient Digestion in Calves

“…Rumen fungi have a strong fibrolytic activity that helps in degradation of low quality roughages by breaking the linkages between lignin and hemicelluloses (Akin et al, 1983;Borneman et al, 1992;Paul et al, 2003). However, the fibre degradation can be affected, as the fungal enzymes are inhibited by the addition of certain microbial inhibitory compounds within the plant structure (Chang and Calza, 2002). Gordon and Phillips (1993) reported that sheep with fungi present in the rumen ate more of a straw based diet because of increased digestibility.…”

Opinion of the Panel on additives and products or substances used in animal feed (FEEDAP) on the safety and efficacy of the product “Amaferm” as a feed additive for dairy cows and cattle for fattening in accordance with Regulation (EC) No 1831/2003

“…The increase in total bacterial numbers is often accompanied by an increase in numbers of cellulolytic bacteria and VFA concentrations in the rumen (Newbold et al, 1992). Protozoal numbers apparently are not affected in the presence of Amaferm, but there are reports of positive effects of the additive on numbers and activity of the rumen fungi (Harper et al, 1996;Chang et al, 1999). It is thought that the increased cell wall degrading activity associated with increased bacterial and fungal numbers accounts, in part at least, for the often observed increase in fibre degradation in the rumen.…”