The Effect of Asiatic Acid and Metformin on The Viability Percentage of Mouse Macrophage Cell Lines RAW264.7 and Mouse Fibroblast Cell Lines NIH3T3

Abstract

Introduction: Diabetes can be caused by inflammatory regulation disorders. Metformin has been reported that inhibits the physiological function of normal cells. Asiatic acid, a bioactive compound from Centella asiatica, has the potential to be developed as a therapeutic agent for diabetes, but little is known about its toxic effects on macrophage cells and fibroblast cells. Objective: The study aims to evaluate the toxic effects of Asiatic acid and metformin on the viability of RAW264.7 macrophage cells and NIH3T3 fibroblast cells. Method: Asiatic acid and metformin with seven concentrations were given to RAW264.7 macrophage cell lines and NIH3T3 fibroblast cell lines. Viability percentage is calculated using the 3- (4, 5-dimethylthiazol-2-il) -2, 5-diphenyltetrazolium bromide method, and the absorbance is measured at 595nm. Results: Results have shown that Asiatic acid with concentrations> 12.5 μg/mL decreases the viability of RAW264.7 and NIH3T3 cells drastically. RAW264.7 and NIH3T3 cells that had been given metformin concentrations of 11.6 μg/mL to 370 μg/mL still showed a large percentage of cell viability. Conclusion: Asiatic acid has shown that the cytotoxic effect is greater than metformin, so it is necessary to pay attention to the concentration of the treatment.

Essential Oils of Two Portuguese Endemic Species of Lavandula as a Source of Antifungal and Antibacterial Agents

“…luisieri EO with high percentages of necrodanes compounds strongly contributes to this high cytotoxicity in fibroblast cells. On the other hand, cell lines have different behavior according to their origin; for example, the same study that used two mouse cell models, RAW 264.7 and fibroblasts, verified that fibroblasts cells were more resistant to the action of the same cytotoxic agents compared to macrophage cells [50]. Due to high cytotoxicity revealed by L. stoechas subsp.…”