The Effect of Ascophyllum nodosum Extract on the Nutraceutical Antioxidant Potential of Vigna radiata Sprout under Salt Stress

Abstract

Mung bean (Vigna radiata L.) sprout is a popular fresh vegetable, tasty and high in antioxidants. To increase yield and quality after the occurrence of both abiotic and biotic stresses, the application of seaweed extracts is of great importance. Hence, this study was conducted to determine the effect of Ascophyllum nodosum extract (ANE) in the presence of salt on the antioxidant potential of V. radiata sprouts. Different concentrations of ANE viz. 0.00, 0.01, 0.05, 0.10, and 0.50% and NaCl 0, 25, 50, 75, and 100 mM alone and in combinations were tested for researching the antioxidant potential of V. radiata sprouts at 0, 24, and 36 h of sprouting. The DPPH free-radical-scavenging activity of sprouts of V. radiata was found to increase with time and peaked at 24 h of treatment. The A. nodosum extract (0.01%) could reverse the ill effect of the low level of salinity posed by up to 25 mM NaCl. The increasing salinity deteriorated the antioxidant activity using ABTS method of sprouts down to 20.45% of the control at 100 mM NaCl. The total phenolic content (TPC), total flavonoid content (TFC), and reducing power of V. radiata sprouts was found to increase till 36 h of sprouting. A slight increase in TPC, TFC and reducing power was observed when seeds were treated with low concentrations of ANE. The elevation in TPC, TFC and reducing power upon treatment with low concentrations of ANE was also noticed in sprouts in saline combinations. Alpha amylase inhibition activity was found to reach a (67.16% ± 0.9) maximum at 24 h of sprouting at a 0.01% concentration of ANE. Tyrosinase inhibition and alpha glucosidase inhibition was 88.0% ± 2.11 and 84.92% ± 1.2 at 36 h of sprouting, respectively, at 0.01% concentration of ANE. A. nodosum extract is natural, environmentally friendly, and safe, and could be used as one of the strategies to decline stress at a low level and enhance the antioxidant activities in V. radiata sprouts, thus increasing its potential to be developed as an antioxidant-based functional food.

Salinity Stress Influences the Main Biochemical Parameters of Nepeta racemosa Lam.

“…Similar behavior has been reported for other plant species such as Cynara scolymus L. [ 49 ], and Verbena officinallis L. [ 50 ]. A concentration of 100 mM NaCl combined with the use of Ascophyllum nodosum led to an increased flavonoid concentration in Vigna radiata L. [ 51 ], while 150 mM had the same stimulating effect for Trigonella foenum-graecum L. [ 52 ], confirming the results presented in this study.…”

Potential Use of Ascophyllum nodosum as a Biostimulant for Improving the Growth Performance of Vigna aconitifolia (Jacq.) Marechal

“…An increase in chlorophyll 'a', chlorophyll 'b', total chlorophyll, and carotenoids in A. esculentus by Rosenvigea intricate [46] and in V. mungo by U. reticulate [8,23] was also observed. The application of A. nodosum was also previously reported to enhance the chlorophyll content in the leaves of Solanum lycopersicum, Hordeum vulgare, Phaseolus vulgaris, Zea mays, V. radiata, and Triticum aestivum [3,47]. Overall, ANE supplied through roots showed better results compared to foliar application of ANE.…”

Sargassum vulgare extract as a bio‐elicitor of natural stilbenes production and antioxidant potential in peanut (Arachis hypogaea L.) sprouts

“…(2019) showed that TPC and TFC increased during germination. Previous studies reported the same positive effect by applying seaweed extract as an elicitor in seeds and crops of legumes (Rathore et al., 2009; Fan et al., 2011; Kocira et al ., 2018a; Kumari et al., 2021), but not in peanut. It was also reported that the polysaccharides present in Ulva lactuca extract contributed to the promotion of phenolic metabolism as evidenced by increased PAL activity and higher TPC and lignin content in olive (Ben Salah et al., 2018), sweet pepper and tomato seedlings (Ali et al., 2021).…”

“…The antioxidant capacity may be influenced by other components that could affect metabolic functions. Other studies have linked anthocyanins, antioxidant amino acids and bioactive peptides to the antioxidant activity of legume sprouts (León‐López et al., 2020; Kumari et al., 2021). It is likely that different antioxidant compounds may act synergistically to lead to significant antioxidant activity after elicitor treatment (Zhang et al., 2020; Chung et al., 2021; Zhu et al., 2021).…”

“…In legumes, the antioxidant activity can be increased by using seaweed‐derived elicitors. The extract of Ascophyllum nodosum on Vigna radiata (Kumari et al., 2021) and Glycine max positively affected their yield, phenolic content, and antioxidant potential (Kocira et al . 2018b).…”